FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation

Baloglu, Fatma Kucuk
Garip, Sebnem
Heise, Sebastian
Brockmann, Gudrun
Severcan, Feride
Obesity is a heterogeneous disorder which increases risks for multiple metabolic diseases, such as type 2 diabetes. The current study aims to characterize and compare visceral and subcutaneous adipose tissues in terms of macromolecular content and investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1) immunohistological staining were used to investigate gonadal (visceral) and inguinal (subcutaneous) adipose tissues of male Berlin fat mice inbred (BFMI) lines, which are spontaneously obese. The results indicated a remarkable increase in the lipid/protein ratio, accompanied with a decrease of UCP1 protein content which might be due to the transdifferentiation of brown adipocytes to white adipocytes in obese groups. It has been widely reported that brown adipose tissue has a strong effect on fatty acid and glucose homeostasis and it could provide an opportunity for the therapy of obesity. When the amount of brown adipose tissue was decreased, lower unsaturation/saturation ratio, qualitatively longer hydrocarbon acyl chain length of lipids and higher amount of triglycerides were obtained in both adipose tissues of mice lines. The results also revealed that subcutaneous adipose tissue was more prone to obesity-induced structural changes than visceral adipose tissue, which could originate from it possessing a lower amount of brown adipose tissue. The current study clearly revealed the power of FTIR microspectroscopy in the precise determination of obesity-induced structural and functional changes in inguinal and gonadal adipose tissue of mice lines.


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Statins are commonly used to control hypercholesterolemia and to prevent cardiovascular diseases. Among the statins, Simvastatin is one of the most frequently prescribed statins because of its efficacy in reducing LDL lipoprotein cholesterol levels, its tolerability, and its reduction of cardiovascular risk and mortality. Conflicting results have been reported with regard to benefits (pleiotropic effects) as well as risks (adverse effects) of simvastatin on different soft and hard tissues. In the current st...
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Boyar, Handan; Turan, Belma; Severcan, Feride (Hindawi Limited, 2003)
Diabetes mellitus (DM) can be accepted as a heterogenous multi organ disorder that can affect various systems of the human body. Disorders include retinopathy, neuropathy, cardiomyopathy, musculoskeletal abnormalities such as diminished bone formation and bone healing retardation. Low bone mineral density is often mentioned as a complication for patients with insulin dependent diabetes mellitus (type I DM). Streptozotocin (STZ) induced diabetic rats are good models for investigation of the complications of ...
FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection
Didonna, Alessandro; Vaccari, Lisa; Bek, Alpan; Legname, Giuseppe (2010-07-01)
Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to replicate into nascent PrPSc from the host-encoded cellular isoform of the prion protein. The present work aims at providing novel insight into cellular response upon prion infection, as evidenced by Synchrotron Radiation InfraRed MicroSpectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was e...
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Bozkurt, Ozlem; Severcan, Mete; Severcan, Feride (2010-01-01)
Diabetes Mellitus (DM) is a metabolic disorder, characterized by abnormally high blood glucose levels due to decreased secretion or effectiveness in function of insulin. Having a role in carbohydrate and lipid metabolism, skeletal muscle is affected by the absence of insulin in diabetic conditions. This current study reports the application of Fourier transform infrared (FTIR) spectroscopy in the determination of macromolecular alterations in streptozotocin (STZ)-induced diabetic rat skeletal Soleus (SOL) m...
Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers
Hua, Serenus; An, Hyun Joo; Özcan Kabasakal, Süreyya; Ro, Grace S.; Soares, Stephanie; DeVere-White, Ralph; Lebrilla, Carlito B. (Royal Society of Chemistry (RSC), 2011-01-01)
Glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases including cancer. Glycan compositional profiling of human serum with mass spectrometry has already identified potential biomarkers for several types of cancer and diseases; however, composition alone does not fully describe glycan stereo-and regioisomeric diversity. The vast structural heterogeneity of glycans presents a formidable analytical challenge. We have developed a method to identify and quantif...
Citation Formats
F. K. Baloglu, S. Garip, S. Heise, G. Brockmann, and F. Severcan, “FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation,” ANALYST, pp. 2205–2214, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57226.