FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection

Date

2010-07-01

Author

Didonna, Alessandro
Vaccari, Lisa
Bek, Alpan
Legname, Giuseppe

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Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to replicate into nascent PrPSc from the host-encoded cellular isoform of the prion protein. The present work aims at providing novel insight into cellular response upon prion infection, as evidenced by Synchrotron Radiation InfraRed MicroSpectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was employed to investigate the biochemical perturbations occurring in prion infected mouse hypothalamic GT1-1 cells at cellular and sub-cellular levels. Monitoring spectral differences allowed to discriminate between infected and uninfected cells, introducing IRMS as a robust diagnostic tool to detect prions in biological samples. By comparing the average spectra of infected and un-infected cells, an increased number of lysosomes in infected cells was postulated, and further confirmed by SR-IRMS at sub-cellular spatial resolution and by fluorescent microscopy. The purpose of this work, therefore, consists in proposing IRMS as a powerful multi-screening platform, drawing on the synergy with conventional biochemical assays to increase the accuracy of investigations performed at the single cell level.

Subject Keywords

prion, PrPSc, infrared microspectroscopy (IRMS), synchrotron radiation (SR), functional group mapping, cluster analysis

URI

https://hdl.handle.net/11511/69969

Journal

PRION

Collections

Department of Physics, Article

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Citation Formats

A. Didonna, L. Vaccari, A. Bek, and G. Legname, “FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection,” PRION, pp. 210–210, 2010, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/69969.