FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection

Date

2010-07-01

Author

Didonna, Alessandro
Vaccari, Lisa
Bek, Alpan
Legname, Giuseppe

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

109
views

0
downloads

Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to replicate into nascent PrPSc from the host-encoded cellular isoform of the prion protein. The present work aims at providing novel insight into cellular response upon prion infection, as evidenced by Synchrotron Radiation InfraRed MicroSpectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was employed to investigate the biochemical perturbations occurring in prion infected mouse hypothalamic GT1-1 cells at cellular and sub-cellular levels. Monitoring spectral differences allowed to discriminate between infected and uninfected cells, introducing IRMS as a robust diagnostic tool to detect prions in biological samples. By comparing the average spectra of infected and un-infected cells, an increased number of lysosomes in infected cells was postulated, and further confirmed by SR-IRMS at sub-cellular spatial resolution and by fluorescent microscopy. The purpose of this work, therefore, consists in proposing IRMS as a powerful multi-screening platform, drawing on the synergy with conventional biochemical assays to increase the accuracy of investigations performed at the single cell level.

Subject Keywords

prion, PrPSc, infrared microspectroscopy (IRMS), synchrotron radiation (SR), functional group mapping, cluster analysis

URI

https://hdl.handle.net/11511/69969

Journal

PRION

Collections

Department of Physics, Article

Suggestions

OpenMETU
Core

Infrared Microspectroscopy: A Multiple-Screening Platform for Investigating Single-Cell Biochemical Perturbations upon Prion Infection Didonna, Alessandro; Vaccari, Lisa; Bek, Alpan; Legname, Giuseppe (American Chemical Society (ACS), 2011-03-01) Prion diseases are a group of fatal neurodegenerative disorders, characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to convert the host-encoded cellular isoform of the prion protein, PrPC, into nascent PrPSc. The present work aims at providing novel insight into cellular response upon prion infection evidenced by synchrotron radiation infrared microspectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was empl...
FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation Baloglu, Fatma Kucuk; Garip, Sebnem; Heise, Sebastian; Brockmann, Gudrun; Severcan, Feride (Royal Society of Chemistry (RSC), 2015-01-01) Obesity is a heterogeneous disorder which increases risks for multiple metabolic diseases, such as type 2 diabetes. The current study aims to characterize and compare visceral and subcutaneous adipose tissues in terms of macromolecular content and investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1) immunohistological staining were used to investigate gonadal (visceral) and inguinal (subcu...
FTIR Spectroscopic Investigation of Mineral Structure of Streptozotocin Induced Diabetic Rat Femur and Tibia Boyar, Handan; Turan, Belma; Severcan, Feride (Hindawi Limited, 2003) Diabetes mellitus (DM) can be accepted as a heterogenous multi organ disorder that can affect various systems of the human body. Disorders include retinopathy, neuropathy, cardiomyopathy, musculoskeletal abnormalities such as diminished bone formation and bone healing retardation. Low bone mineral density is often mentioned as a complication for patients with insulin dependent diabetes mellitus (type I DM). Streptozotocin (STZ) induced diabetic rats are good models for investigation of the complications of ...
FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media Haris, PI; Severcan, Feride (1999-09-15) With increasing use of proteins in many different applications, ranging from phramaceuticals to biosensors and biomaterials, there has emerged a need for protein structural characterisation in diverse environments. In many cases it is not sufficient to just have the three-dimensional structure of a protein in H2O or in the crystalline state. Often information on the structural properties of a protein is required in the presence of organic solvents, detergent micelles, phospholipid membranes and so on. Fouri...
Structural Insights into Alternate Aggregated Prion Protein Forms POLANO, maurizio; Bek, Alpan; BENETTİ, federico; lazzarino, marco; LEGNAME, giuseppe (Elsevier BV, 2009-11-13) The conversion of the cellular form of the prion protein (PrPC) to an abnormal, alternatively folded isoform (PrPSc) is the central event in prion diseases or transmissible spongiform encephalopathies. Recent studies have demonstrated de novo generation of murine prions from recombinant prion protein (recPrP) after inoculation into transgenic and wild-type mice. These so-called synthetic prions lead to novel prion diseases with unique neuropathological and biochemical features. Moreover, the use of recPrP i...

Citation Formats

A. Didonna, L. Vaccari, A. Bek, and G. Legname, “FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection,” PRION, pp. 210–210, 2010, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/69969.