Arylamine N-acetyltransferase activities in human breast cancer tissues

Date

2001-01-01

Author

Geylan, YS
Sak, SD
Güray, Nülüfer Tülün

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

49
views

0
downloads

N-Acetyltransferase activities were determined in tumor (12 malignant and 6 benign) and control (non-cancerous) breast tissues from 18 female patients. The activities of matched 12 malignant tumor and control tissue cytosols showed 6 rapid, 4 intermediate and 2 slow acetylators based on p-aminobenzoic acid (NAT1) and sulfamethazine (NAT2) as substrates, Compared to the activities of slow acetylators, the rapid acetylators exhibited mean apparent V-max values about 5- and 50-fold greater for p-aminobenzoic acid and sulfamethazine, respectively. No correlation was observed between the blood and breast tissue N-acetyltransferase (NAT1 and NAT2) activities. When the mean apparent N-acetyltransferase activities of the malignant and benign breast tumor tissues were compared, the results showed an increased activity for both p-aminobenzoic acid (PABA) and sulfamethazine (SMZ) acetylation in the malignant tissues compared to benign ones, and also control tissues showed lower activities compared to tumor tissues. Moreover, the mean NAT2 activity was about 2-fold greater in the malignant tissues when compared to NAT1 activity.

Subject Keywords

N-acetyltransferase, N-acetylation, NAT1, NAT2, Breast cancer

URI

https://hdl.handle.net/11511/53121

Journal

NEOPLASMA

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Implementation of a fast simulation tool for the analysis of contrast mechanisms in HMMDI and enhancement of the SNR in the experimental set-up İrgin, Ümit; Gençer, Nevzat Güneri; Top, Can Barış; Department of Electrical and Electronics Engineering (2021-9-06) Clinical method for breast tumor detection is Mammography (X-rays), which have limitations and may yield inaccurate results. Alternative novel techniques are required to characterize the breast tissues and extract accurate information for identification of malignancies in the tissue. Harmonic Motion Microwave Doppler Imaging (HMMDI), which enhances hybridizing microwave signals and ultrasound techniques, has been recently proposed for detection of tumors in the tissue. In HMMDI method, the data is a combina...
Comparison of NAT1, NAT2 & GSTT2-2 activities in normal and neoplastic human breast tissues Geylan-SU, YS; Isgor, B; Coban, T; Kapucuoglu, N; Aydintug, S; Iscan, M; Iscan, M; Güray, Nülüfer Tülün (American Society for Pharmacology & Experimental Therapeutics (ASPET), 2006-01-01) In this study, arylamine N-acetyltransferases, NATs (E.C.2.3.1.5) and glutathione-S-transferase-T2-2, GSTT2-2 (E.C.2.5.1.18) enzyme activities in the breast tumor and surrounding tumor-free tissues of 22 female breast cancer patients with infiltrating ductal carcinoma were measured. The possible impacts of grade of malignancy, chemotherapy treatment, estrogen receptor status and menopausal status on all enzyme activities were evaluated. The results showed that, both NAT2 and GSTT2-2 display significant diff...
Comparison of GST Isoenzyme Expression in Normal and Neoplastic Breast Tissue: Correlation with Clinical and Prognostic Factors OĞUZTÜZÜN, SERPİL; İşcan, Mesude; ÖZHAVZALI, MÜZEYYEN; Sak, Serpil Dizbay (2009-01-01) Glutathione S-transferases in breast tissue play an important role in the susceptibility to the mutagenic effects of chemical carcinogens and in the response of breast tumors to chemotherapy. In this study the immunohistochemical staining characteristics of glutathione S-transferase isoenzymes (alpha, mu, pi, and theta) were investigated in invasive duct carcinomas and in normal breast tissue of 43 patients. The relationships between the expression of the GST isoenzymes and some clinicopathological features...
Selenium doped calcium phosphate biomimetic coating on Ti6Al4V orthopedic implant material for anti-cancer and anti-bacterial purposes Yılmaz, Bengi; Evis, Zafer; Banerjee, Sreeparna; Department of Biomedical Engineering (2014) In order to prevent recurrent osteosarcoma in patients undergone surgical resection for implant insertion and infection around implant site, which is a very common complication after surgery, it is very important to functionalize the surface. In this study, to combine the effective antioxidant and potential anti-cancer and anti-bacterial properties of selenium with the high biocompatibility and bioactivity of hydroxyapatite, selenium was added to the structure of hydroxyapatite via biomimetic method and coa...
Magnetic Half Generation PAMAM Dendrimeric Nanoparticles for Targeting Delivery of Gemcitabine Maryam, Parsian; Mutlu, Pelin; Yalçın Azarkan, Serap; Tezcaner, Ayşen; Gündüz, Ufuk (2015-06-09) Antitumor activity of Gemcitabine, a nucleoside analogue, has been reported in a variety of human tumors, including breast cancer in both experimental and clinical studies. Conventional chemotherapeutic agents are unspecifically distributed all over the body where they affect both cancerous and normal cells. They cause major systemic toxicities and drug resistance, which also restricts drugs therapeutic efficacy. Recent advances in nanotechnology have explored new targeting strategies for enhancing intra-tu...

Citation Formats

Y. Geylan, S. Sak, and N. T. Güray, “Arylamine N-acetyltransferase activities in human breast cancer tissues,” NEOPLASMA, pp. 108–111, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53121.