Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) based tissue engineering matrices

Date

2003-02-01

Author

Kose, GT
Ber, S
Korkusuz, Feza
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

145
views

0
downloads

In this study, the aim was to produce tissue-engineered bone using osteoblasts and a novel matrix material, poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV). In order to prepare a porous PHBV matrix with uniform pore size, sucrose crystals were loaded in the foam and then leached leaving pores behind. The surface of the PHBV matrix was treated with rf-oxygen plasma to increase the surface hydrophilicity. SEM examination of the PHBV matrices was carried out. Stability of PHBV foams in aqueous media was studied. The pH decrease is an indication of the degradation extent. The weight and density were unchanged for a period of 120 days but then a significant decrease was observed for the rest of the study. Osteoblast cells were then isolated from rat bone marrow and seeded onto PHBV matrices. The metabolization and proliferation on the foams was determined with MTS assay which showed that osteoblasts proliferated on PHBV. It was also found that cells proliferated better on large pore size foams (300-500 mum) than on the small pore size foams (75-300 mum). Production of ALP was measured spectrophotometrically. The present study demonstrated that PHBV matrices are suitable substrates for osteoblast proliferation and differentiation. (C) 2003 Kluwer Academic Publishers.

Subject Keywords

Hydroxybutyrate-hydroxyvalerate copolymers, Polyhydroxybutyrate-co-hydroxyvalerate, Biodegradable medical devices, In-vivo degradation, Vitro, Biocompatibility, Polymers, Culture

URI

https://hdl.handle.net/11511/54487

Journal

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

Collections

Department of Physical Education and Sports, Article

Suggestions

OpenMETU
Core

Poly(epsilon-caprolactone) Composite Scaffolds Loaded with Gentamicin-Containing beta-Tricalcium Phosphate/Gelatin Microspheres for Bone Tissue Engineering Applications Sezer, Umran Aydemir; Arslantunalı Şahin, Damla; Aksoy, Eda Ayse; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2014-04-15) In this study, novel poly(epsilon-caprolactone) (PCL) composite scaffolds were prepared for bone tissue engineering applications, where gentamicin-loaded -tricalcium phosphate (-TCP)/gelatin microspheres were added to PCL. The effects of the amount of -TCP/gelatin microspheres added to the PCL scaffold on various properties, such as the gentamicin release rate, biodegradability, morphology, mechanical strength, and pore size distribution, were investigated. A higher amount of filler caused a reduction in th...
Poly(lactic acid) based nanocomposites: mechanical, thermal and rheological properties and morphology Açık, Eda; Yılmazer, Ülkü; Department of Chemical Engineering (2014) The aim of this study was to increase the potential applications of poly(lactic acid) (PLA) by incorporating reactive functionalities with different nano-scale fillers. To investigate the effects of nanoclay types, five different organically modified nanoclays (Cloisites®15A, 25A and 30B and Nanofils®5 and 8) were used. Two elastomeric compatibilizers, ethylene-glycidyl methacrylate (E-GMA) and ethylene-butyl acrylate-maleic anhydride (E-BA-MAH), were added to the nanocomposites produced via melt compoundin...
Poly(epsilon-caprolactone) composites containing gentamicin-loaded beta-tricalcium phosphate/gelatin microspheres as bone tissue supports Sezer, Umran Aydemir; Aksoy, Eda Ayse; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2013-02-05) In this work, novel antibacterial composites were prepared by using poly(epsilon-caprolactone) (PCL) as the main matrix material, and gentamicin-loaded microspheres composed of beta-tricalcium phosphate (beta-TCP) and gelatin. The purpose is to use this biodegradable material as a support for bone tissue. This composite system is expected to enhance bone regeneration by the presence of beta-TCP and prevent a possible infection that might occur around the defected bone region by the release of gentamicin. Th...
Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells Kızıltay, Aysel; San Roman, Julio; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2015-08-01) The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and advanced bioplotting techniques. The resulting scaffolds were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), me...
Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06) This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...

Citation Formats

G. Kose, S. Ber, F. Korkusuz, and V. N. Hasırcı, “Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) based tissue engineering matrices,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, pp. 121–126, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54487.