Phylogeography and Population Genetics of Honey Bees (Apis mellifera) From Turkey Based on COI-COII Sequence Data

Solorzano, Cesar D.
Szalanski, Allen L.
Kence, Meral
McKern, Jackie A.
Austin, James W.
Kence, Aykut
A study that involved DNA sequencing of COI-COII intergenic region of the mitochondrial DNA genome of Apis mellifera honey bees from Turkey was conducted to determine the population genetics and phylogeographic structure of this species from seven distinct areas of Turkey. From the 132 honey bees subjected to DNA sequencing, a total of 12 mitotypes of A. mellifera "C" lineage were observed, of which only one mitotype, C 13, had been reported previously. The most common mitotype, C12, accounted for 47% of the Apis mellifera "C" lineage samples and was found in 13 of the 22 sampled locations. This mitotype was also the basal ancestral mitotype based on TCS spanning tree analysis. The greatest amount of genetic diversity was observed in Bursa, where 4 mitotypes of the A. mellifera "C" lineage were unique to this location. Wright's F-statistics revealed that Artvin and Bursa were the most genetically distinct locations relative to the other sampled locations. Applying a molecular clock, Turkish A. mellifera "C" lineage mitotypes have been diverging for approximately 10,000 to 16,500 yr. based on phylogenetic analysis. In addition, two A. m. syriaca samples were observed from Hatay, Turkey. Phylogenetic analysis which included other A. mellifera subspecies confirms the subspecies relationships of A. mellifera "C" lineage, and A. m. syriaca. this study corroborates other studies that show Turkey to be a reservoir of genetically distinct populations of A. mellifera "C" lineage, which can be useful for developing genetic conservation strategies for A. mellifera.


Genetic structure of honeybee, Apis mellifera L. (Hymenoptera : Apidae) populations of Turkey inferred from microsatellite analysis
Bodur, Cagri; Kence, Meral; Kence, Aykut (2007-01-01)
We analyzed the genetic structure of 11 honeybee (Apis mellifera L) populations from Turkey and one from Cyprus using 9 microsatellite marker loci. Heterozygosity levels, mean number of alleles per population, number of diagnostic alleles, and pairwise F-ST values were computed for the populations studied. Heterozygosity levels were found to range between 0.54 and 0.68. We detected high levels of genetic divergence among the populations based on pairwise F-ST values; 50 of 66 pairwise F-ST values were signi...
Phylogeny and genetic variation of Reticulitermes (Isoptera : Rhinotermitidae) from the eastern Mediterranean and Middle East
Austin, James W.; Szalanski, Allen L.; Ghayourfar, Rahim; Kence, Aykut; Gold, Roger E. (2006-01-01)
Recent genetic evaluations of R. lucifugus from France and Italy with R. lucifugus from Turkey suggests that these geographic groups likely constitute separate species. A more extensive review of eastern Mediterranean termites is needed to clarify their respective classifications. DNA sequencing analysis of the mitochondrial 16S rRNA region was used to examine genetic variation in the termite genus Reticulitermes Holmgren from eastern Europe and the Middle East. Sequencing of a 438 bp region from 39 individ...
Phylogeography of Turkish Honeybees (Apis mellifera L.) Based on mtDNA Sequences
Çelikgil, Atila; Kükrer, Mert; Bilgin, Cemal Can (null; 2019-07-09)
There are 26 recognized subspecies of honeybees throughout the world and 5 of them are distributed in Turkey. Due to the migratory beekeepers and queen trade practices in addition to other anthropogenic factors, population structure of these 5 subspecies are under threat and require further research followed by immediate establishment of conservation strategies. In this study, we aimed to determine the phylogeography and biodiversity of Turkish Honeybees. In order to assess genetic variability and populatio...
Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey
Kandemir, I; Kence, M; Kence, Aykut (Springer Science and Business Media LLC, 2000-05-01)
Six enzyme systems were studied to determine the genetic variability in honeybee populations in Turkey. Ten morphometric characters were also measured to determine the extent of morphometric variation. Out of six enzyme systems, four were found to be polymorphic with 16 allozymes. The average heterozygosity was calculated as 0.072 +/- 0.007. Morphometric and electrophoretic variables were equally effective in discriminating honeybee populations. European and Anatolian honeybees were separated on the first a...
Ivanova, Evgeniya N.; Petrov, Plamen; Bouga, Maria; Emmanouel, Nicholas G.; TUNCA, RAHŞAN İVGİN; Kence, Meral (2010-01-01)
Genetic variation of honey bee populations from 9 different locations of Bulgaria was studied using 4 enzymic systems (MDH, ME, EST and ALP) corresponding to 4 genetic loci and PCR-RFLP's analysis of 16s rDNA, COI, and ND5 gene segments of mtDNA. Allozyme analysis revealed that all loci were polymorphic in almost all studied populations. The observed heterozygosity was found to range from 0.146 to 0.258, and Nei's genetic distance between 0.006 and 0.057 among the populations. Bulgarian honey bees are clust...
Citation Formats
C. D. Solorzano, A. L. Szalanski, M. Kence, J. A. McKern, J. W. Austin, and A. Kence, “Phylogeography and Population Genetics of Honey Bees (Apis mellifera) From Turkey Based on COI-COII Sequence Data,” SOCIOBIOLOGY, pp. 237–246, 2009, Accessed: 00, 2020. [Online]. Available: