Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

Date

2012-12-01

Author

Kayihan, C.
EYİDOĞAN, FÜSUN
Afsar, N.
Öktem, Hüseyin Avni
YÜCEL, MUSTAFA

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

109
views

0
downloads

The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (-3 A degrees C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (-7 A degrees C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.

Subject Keywords

Plant Science, Horticulture

URI

https://hdl.handle.net/11511/44161

Journal

BIOLOGIA PLANTARUM

DOI

https://doi.org/10.1007/s10535-012-0143-x

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Reciprocal effects in anther cultures of wheat hybrids YILDIRIM, MEHMET; Bahar, B.; Genc, I.; HATİPOĞLU, RÜŞTÜ; Altintas, S. (Institute of Experimental Botany, 2008-12-01) This study was conducted to determine the reciprocal effects for anther culture response in wheat (Triticum aestivum L.) using a set of 4 x 4 full diallel crosses. Both reciprocal and nuclear genetic effects were highly significant for anther culture response and useful for selection and breeding purposes. General combining ability (GCA) effects were predominant for all investigated anther culture traits. Also, significant differences for specific combining ability (SCA) effects were detected between recipr...
Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test Oz, M. T.; TURAN, ÖZLEM; Kayihan, C.; EYİDOĞAN, FÜSUN; EKMEKÇİ, YASEMİN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Institute of Experimental Botany, 2014-12-01) The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durati...
Superoxide dismutase activity of hexaploid and tetraploid wheat cultivars subjected to heat and chilling stress Eyidogan, F; Öktem, Hüseyin Avni; Yucel, M (2003-01-01) Effect of heat (37degreesC, 24 hours) and chilling (4degreesC, 24 hours) stress on SOD activity was investigated in shoots and roots of hexaploid and tetraploid wheat cultivars. In order to analyse the changes of SOD isozymes against heat and chilling stress, shoot and root extracts were subjected to native PAGE. Extracts of both tissues revealed the presence of Mn and Cu/Zn isozymes of SOD. Cu/ZnSOD activity comprised 90% of total SOD activity in both tissues. Heat stress caused a significant increase in S...
Nitrate reductase and glutamate dehydrogenase activities of resistant and sensitive cultivars of wheat and barley under boron toxicity Mahboobi, H; Yucel, M; Öktem, Hüseyin Avni (2002-01-01) In this study, changes in activity of key enzymes of nitrogen assimilation, namely nitrate reductase and glutamate dehydrogenase upon boron (B) toxicity in wheat (Triticum aestivum) and barley (Hordeum vulgare) cultivars were investigated. Ten-day-old seedlings were exposed to 10 mM boric acid for a duration of five days. Plants growing on the nutrient solutions and receiving no excess B, were maintained as controls. All experiments were conducted on leaf and root tissues of control and B-treated seedlings ...
The effect of hybrid vigor on callus induction and plant regeneration from mature embryo culture of barley (Hordeum vulgare) Ozgen, M; Birsin, MA; Onde, S (Springer Science and Business Media LLC, 2005-07-01) The influence of hybrid vigor on callus induction, callus weight, regeneration capacity of callus, culture efficiency and number of regenerated plants from mature embryo cultures of winter barley ( Hordeum vulgare L.) hybrid was studied. A total of 15 F-1 hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis was noticed for all these characters except for callus weight. Plants regenerated in vitro were successfully established...

Citation Formats

C. Kayihan, F. EYİDOĞAN, N. Afsar, H. A. Öktem, and M. YÜCEL, “Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars,” BIOLOGIA PLANTARUM, pp. 693–698, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44161.