Analysis of Molecular Resistance Mechanisms in Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera) Populations Under Pyrethroid Stress in Turkey

Date

2014-01-01

Author

Konuş, Metin
UGURLU KARAAĞAÇ, SAKİNE
İşcan, Mesude

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

97
views

0
downloads

Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a major pest of economically important crops such as cotton, tomato and soybean. In order to control this pest, pyrethroid insecticides have been extensively used in farming areas all over the world. However, applications of excessive amounts of these insecticides can result in resistance development in the field populations of H. armigera. Resistance to the pyrethroids, beta-cypermethrin and lambda cyhalothrin, was analysed using bioassays. It was found that a canakkale field population of H. armigera field showed low (2.1-fold) and moderate (14.5-fold) resistance levels for beta-cypermethrin and lambda-cyhalothrin, respectively. Furthermore, expressions of selected CYP450, GST and esterase genes of H. armigera canakkale field populations were compared to those of a susceptible strain using real-time PCR. Our results indicate that H. armigera reacts to pyrethroids mainly by increasing expression levels of CYP450s such as CYP9Al2 and CYP9A14. However, GST and esterase genes expression levels were not significantly altered in a field population. GSTs and esterases were also analyzed using biochemical assays. While GSTs and esterase genes were not found to be up-regulated in the real-time PCR, except GST-DCNB activity, the biochemical assays also showed no significant increases in enzyme activities in the canakkale field population as compared to the susceptible strain. Consequently, CYP9Al2 and CYP9A14 together with certain GSTs, catalyzing DCNB substrate, are proposed to be involved in the metabolic responses against beta-cypermethrin and lambda-cyhalothrin insecticides in field population of H. armigera from Turkey.

Subject Keywords

Helicoverpa armige, Pyrethroid insecticides, QRT-PCR, CYP450, Esterases and glutathione s-transferases

URI

https://hdl.handle.net/11511/54841

Journal

JOURNAL OF THE ENTOMOLOGICAL RESEARCH SOCIETY

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Real-time PCR analysis of pyrethroid resistance in Helicoverpa armigera from Turkey Konuş, Metin; UGURLU KARAAĞAÇ, SAKİNE; İşcan, Mesude (2014-01-01) Aim: Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a polyphagous pest of a wide range of crops such as cotton, tomato and soybean. Pyrethroid insecticides have commonly used against it in agricultural areas, but excess amount applications of them result in resistance development in the field populations of H. armigera. Resistance development usually occurs with increased metabolism of certain enzymatic systems such as CYP450, GST and esterases. Therefore, expressions of selected CYP450, GST and ...
Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13) Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...
Investigating the effect of different factors on enzymatic hydrolysis of sugar beet pulp & corn cob Leyluhan Yurtseven, Berna; Öztop, Halil Mecit; Department of Biotechnology (2019) Hydrolysis of biomass obtained as the waste of industrial or agricultural production has gained significant interest in recent years. In this study, 2 different biomasses were studied. In the 1st part of the study, sugar beet pulp (SBP) hydrolysis was optimized using Response Surface Methodology (RSM). Sugar beet pulp is an important by product of sugar manufacturing and its hydrolysis could yield valuable sugars that can be used in fermentation processes. As the parameters of SBP hydrolysis by RSM, effect ...
Molecular adaptations of Helicoverpa armigera midgut tissue under pyrethroid insecticide stress characterized by differential proteome analysis and enzyme activity assays Konus, Metin; Koy, Cornelia; Mikkat, Stefan; Kreutzer, Michael; Zimmermann, Ralf; İşcan, Mesude; Glocker, Michael O. (Elsevier BV, 2013-06-01) Helicoverpa armigera is an insect that causes important economic losses in crops. To reduce this loss, pyrethroids have been commonly used against H. armigera in farming areas. However, excess and continuous usage of pyrethroids cause resistance in H. armigera. Therefore, expressions of midgut proteins of two H. armigera field populations were compared to those of a susceptible strain by 2-D PAGE and MALDI-ToF-MS. Our results indicate that H. armigera reacts to pyrethroid-induced stress mainly by increasing...
POTENTIAL BIOMONITORING USE OF VARIATIONS IN ESTERASE, GLUTATHIONE S-TRANSFERASE, AND ACETYLCHOLINESTERASE ACTIVITIES IN Musca domestica L. TAŞKIN, VATAN; Taskin, Belgin Gocmen; KÜÇÜKAKYÜZ, KÖKSAL; Kence, Meral (2009-01-01) Many ecosystems have been under the continuous influx of both organic and inorganic xenobiotics, including organophosphorus compounds, as a result of intense agricultural and industrial activities. Biochemical biomarkers have been increasingly used in determining the effects of xenobiotics in studies aimed at assessing ecological risks on ecosystems. In this study, as a model organism, housefly (Musca domestica L.) samples collected from 48 different locations belonging to 16 provinces in Aegean and Mediter...

Citation Formats

M. Konuş, S. UGURLU KARAAĞAÇ, and M. İşcan, “Analysis of Molecular Resistance Mechanisms in Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera) Populations Under Pyrethroid Stress in Turkey,” JOURNAL OF THE ENTOMOLOGICAL RESEARCH SOCIETY, pp. 81–92, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54841.