Real-time PCR analysis of pyrethroid resistance in Helicoverpa armigera from Turkey

Date

2014-01-01

Author

Konuş, Metin
UGURLU KARAAĞAÇ, SAKİNE
İşcan, Mesude

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

82
views

0
downloads

Aim: Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a polyphagous pest of a wide range of crops such as cotton, tomato and soybean. Pyrethroid insecticides have commonly used against it in agricultural areas, but excess amount applications of them result in resistance development in the field populations of H. armigera. Resistance development usually occurs with increased metabolism of certain enzymatic systems such as CYP450, GST and esterases. Therefore, expressions of selected CYP450, GST and esterase genes of H. armigera field populations (Adana and Mardin) were compared to those of a susceptible strain by real-time PCR method for analyzing role of these systems in pyrethroid resistance development of H. armigera.

Subject Keywords

Helicoverpa armigera, qRT-PCR, CYP450, Pyrethroid insecticides, Resistance

URI

https://hdl.handle.net/11511/57118

Journal

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

DOI

https://doi.org/10.5505/tjb.2014.50490

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Analysis of Molecular Resistance Mechanisms in Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera) Populations Under Pyrethroid Stress in Turkey Konuş, Metin; UGURLU KARAAĞAÇ, SAKİNE; İşcan, Mesude (2014-01-01) Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a major pest of economically important crops such as cotton, tomato and soybean. In order to control this pest, pyrethroid insecticides have been extensively used in farming areas all over the world. However, applications of excessive amounts of these insecticides can result in resistance development in the field populations of H. armigera. Resistance to the pyrethroids, beta-cypermethrin and lambda cyhalothrin, was analysed using bioassays. It was f...
Evaluation of cotton stalk hydrolysate for xylitol production Sapci, Burcu; AKPINAR, ÖZLEM; Bolukbasi, Ufuk; Yılmaz, Levent (2016-01-01) Cotton stalk is a widely distributed and abundant lignocellulosic waste found in Turkey. Because of its rich xylose content, it can be a promising source for the production of xylitol. Xylitol can be produced by chemical or biotechnological methods. Because the biotechnological method is a simple process with great substrate specificity and low energy requirements, it is more of an economic alternative for the xylitol production. This study aimed to use cotton stalk for the production of xylitol with Candid...
Application of virus induced gene silencing of brachypodium distachyon, a model organism for crops Demircan, Turan; Akkaya, Mahinur S.; Department of Biochemistry (2009) Grass family is most important family in plant kingdom due to intensive usage of crops in agriculture. To date, molecular biology researches on grass family have had limitations because of inappropriate characteristics of barley and wheat to conduct experiments on them. Brachypodium distachyon that belongs to grass family has recently emerged as a model organism for crops. It shares common characteristics for a model plant due to its small genome, small physical plant size, a short lifecycle, and less deman...
Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13) Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...
Functional analysis of a mirna putatively involved in powdery mildew disease susceptibility in barley Dağdaş, Gülay; Akkaya, Mahinur S.; Department of Biotechnology (2009) Barley is one of the most important crop species in Turkey and powdery mildew is one of the most common pathogen decreasing yield in barley. For this problem, agricultural biologists apply breeding technologies in order to select and propagate resistant barley cultivars. However, this is not a permanent solution since pathogens evolve rapidly to overcome plant resistance mechanisms. On the other hand, molecular plant pathologists are trying to understand basic mechanisms underlying plant-pathogen interactio...

Citation Formats

M. Konuş, S. UGURLU KARAAĞAÇ, and M. İşcan, “Real-time PCR analysis of pyrethroid resistance in Helicoverpa armigera from Turkey,” TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, pp. 176–180, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57118.