Optimization of microwave-assisted extraction of phenolic compounds from tomato: Characterization by FTIR and HPLC and comparison with conventional solvent extraction

Date

2021-03-01

Author

Baltacıoğlu, Hande
Baltacıoğlu, Cem
Okur, İlhami
Tanrıvermiş, Arif
Yalıç, Muharrem

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

373
views

0
downloads

© 2021 Elsevier B.V.In the extraction of phenolic compounds from tomato, Microwave-Assisted Extraction (MAE) was used as a novel technology in this study and compared to conventional solvent extraction (CSE). The optimal extraction conditions for tomato were determined using the multi-level factorial design. For this purpose, different powers (360, 600 and 900 W) and times (30, 60 and 90 s) were used for MAE and also different temperatures (40, 50 and 60 °C) and times (10, 20 and 30 min) were used for CSE. Total phenolic content (TPC), antioxidant activity (AA), and morphological changes were measured by the Folin–Ciocalteu assay, DPPH-scavenging activity, scanning electron microscopy (SEM) respectively. Phenolic compounds of tomato extracts were determined by using Fourier transform infrared (FTIR) spectroscopy and high performance liquid chromatography (HPLC). According to the results, MAE increased TPC and AA compared to CSE. The highest TPC (436.20 ± 4.34 mg GAE/kg FW) and antioxidant activity (39.15 ± 0.91 % of DPPH inhibition) was observed for MAE at 900 W, 90 s which was related with morphological changes, determined by SEM. Multivariate analysis (PCA and PLS) were used to discriminate and construct chemometric models in order to predict AA or TPC of the extracts using IR spectra. The bands found in the FTIR spectrum were found to be associated with chlorogenic acid and ferulic acid. Furthermore, chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid were quantified with HPLC. Chlorogenic acid was detected as the main phenolic compound in tomato extracts. The highest amount of chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid was found as 52.33 ± 0.80, 5.03 ± 0.37, 0.803 ± 0.017 and 0.506 ± 0.013 mg/kg FW, respectively with MAE at 900 w 90 s.

Subject Keywords

Spectroscopy

URI

https://hdl.handle.net/11511/88505

Journal

Vibrational Spectroscopy

DOI

https://doi.org/10.1016/j.vibspec.2020.103204

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Optical characterization of silicon based hydrogenated amorphous thin films by un-visible and infrared measurements Kılıç, İlker; Katırcıoğlu, Bayram; Department of Physics (2006) Various carbon content hydrogenated amorphous silicon carbide (a-Si1ŁxCx:H) and hydrogenated amorphous silicon (a-Si:H) thin films have been deposited on various substrates by using plasma enhanced chemical vapour deposition (PECVD) technique. Transmission spectra of these films have been determined within UV-Visible region and the obtained data were analysed to find related physical constants such as; refractive indices, thicknesses, etc. Fourier transform infrared (FT-IR) spectrometry technique has been u...
The characterization of bacteria with Fourier Transform Infrared (FTIR) spectroscopy Garip, Şebnem; Severcan, Feride; Department of Biology (2005) New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and i...
Optimization of ETV-ICP(TOF)MS and transient signal profiles for reducing isobaric interferences Ertaş, Gülay (Royal Society of Chemistry (RSC), 2005-01-01) One of the advantages of the ETV sample introduction is the ability to temporally separate analyte elements in complex mixtures by differences in their vaporization temperatures within the ETV for ICPMS. However, the broadening of the transient peaks in the transport tubing often obscures this temporal resolution. This study shows that decreasing the transport tubing diameter produces little broadening beyond that produced during aerosol production in the ETV. Maintaining such narrow peaks through the trans...
Photoluminescence study of ge-implanted gase and inse single crystals grown by bridgman method Bilgi, Seda; Akınoğlu, Bülent Gültekin; Department of Physics (2006) In this study, photoluminescence properties of as grown, Ge implanted GaSe and InSe crystals with doses 1013, 1014, and 1015 ions/cm2 and 1015 ions/cm2 Ge implanted and annealed GaSe and InSe single crystals grown by using 3-zone vertical Bridgman-Stockbarger system have been studied by photoluminescence spectroscopy (PL). PL spectra of as grown and implanted GaSe samples with three different doses have been studied in the ranges within the wavelength interval 570-850 nm and in the temperature ranges betwee...
Optimization of supercritical CO2 extraction of Thymbra spicata oil Sonsuzer, S; Şahin, Serpil; Yılmaz, Levent (Elsevier BV, 2004-07-01) The supercritical fluid extraction (SFE) of aroma compounds from Thymbra spicata was studied. Response surface methodology (RSM) was used to optimize the parameters of the supercritical fluid extraction. Independent variables were temperature (40, 50 and 60 degreesC), pressure (80, 100 and 120 bar) and time (30, 60 and 90 min). Dependent variables were yield, monoterpene, sesquiterpene and oxygenated monoterpene contents.

Citation Formats

H. Baltacıoğlu, C. Baltacıoğlu, İ. Okur, A. Tanrıvermiş, and M. Yalıç, “Optimization of microwave-assisted extraction of phenolic compounds from tomato: Characterization by FTIR and HPLC and comparison with conventional solvent extraction,” Vibrational Spectroscopy, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/88505.