Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering

Date

2020-11-01

Author

Rad, Reza Moonesi
ALSHEMARY, AMMAR ZEIDAN GHAILAN
Evis, Zafer
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

376
views

0
downloads

In this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B(2)O(3)incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, porosity, mechanical properties, degradation and bioactivity of the scaffolds was investigated. Human dental pulp stem cells (hDPSCs) were enzymatically isolated and used for cell culture studies. According to scanning electron microscope analysis, the porous structure of the scaffolds was preserved after polymer coating. After polymer infiltration, the porosity of the scaffolds decreased from 64.2% to 59.35% for pure BG scaffolds and from 67.3% to 58.9% for B2O3-doped scaffolds. Meanwhile, their compressive strengths increased from 0.13 to 0.57 MPa and from 0.20 to 0.82 MPa, respectively. After polymer infiltration, 7% B2O3-incorporated BG scaffolds had higher weight loss and Ca-P layer deposition than pure BG scaffolds, after 14 d of incubation in simulated body fluid at 37 degrees C. Higher attachment and proliferation of hDPSCs were observed on 7% B2O3-BG-CA/GE scaffolds. In addition, the alkaline phosphatase activity of the cells was about 1.25-fold higher in this group than that observed on BG-CA/GE scaffolds after 14 d of incubation in osteogenic medium, while their intracellular calcium amounts were 1.7-fold higher than observed on BG-CA/GE after 7 d of incubation in osteogenic medium. Our results suggested that porous cellulose acetate-gelatin-coated boron-BG scaffolds hold promise for bone tissue engineering applications.

Subject Keywords

Bioengineering, Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/56307

Journal

BIOMEDICAL MATERIALS

DOI

https://doi.org/10.1088/1748-605x/ab8d47

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Biodegradable polymer promotes osteogenic differentiation in immortalized and primary osteoblast-like cells Onat, Bora; Tuncer, Sinem; Ulusan, Sinem; Banerjee, Sreeparna; Erel Göktepe, İrem (IOP Publishing, 2019-07-01) Biodegradable polymers have been broadly used as agents that can complex with and deliver osteoinductive agents, but osteoinductivity of the polymers themselves has been rarely studied. Here we report the osteoinductivity of poly(4-hydroxy-L-proline ester) (PHPE), a biodegradable cationic polymer with cell penetrating properties. Under physiological conditions, PHPE degrades into trans-4-hydroxy-L-prohne (trans-Hyp), a non-coded amino acid with essential functions in collagen fibril formation and fibril sta...
Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces HAKKI, SEMA; Bozkurt, S. Buket; HAKKI, ERDOĞAN EŞREF; KORKUSUZ, PETEK; PURALI, NUHAN; Koc, Nursen; Timucin, Muharrem; ÖZTÜRK, ADNAN; Korkusuz, Feza (IOP Publishing, 2012-08-01) mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and...
Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering Türkkan, Sibel; Atila, Deniz; Akdağ, Akın; Tezcaner, Ayşen (Wiley, 2018-10-01) In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnec...
Development of electrically conductive porous silk fibroin/CNF scaffolds. Tufan, Yiğithan; Garipcan, B; Ercan, B (IOP Publishing, 2020-10-22) Tissue engineering applications typically require 3D scaffolds which provide requisite surface area for cellular functions, while allowing nutrient, waste and oxygen transportation with the surrounding tissues. Concurrently, scaffolds should ensure sufficient mechanical properties to provide mechanically stable frameworks under physiologically relevant stress levels. In the meantime, electrically conductive platforms are also desired for the regeneration of specific tissues, where electrical impulses are tr...
Immobilization of glucose oxidase onto gelatin for biosensor construction Emregul, E; Sungur, S; Akbulut, Ural (Informa UK Limited, 2005-01-01) The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized usin...

Citation Formats

R. M. Rad, A. Z. G. ALSHEMARY, Z. Evis, D. Keskin, and A. Tezcaner, “Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering,” BIOMEDICAL MATERIALS, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56307.