IN-VIVO EFFECTS OF THE ANESTHETIC, BENZOCAINE, ON LIVER MICROSOMAL CYTOCHROME-P450 AND MIXED-FUNCTION OXIDASE ACTIVITIES OF GILTHEAD SEABREAM (SPARUS-AURATA)

Date

1994-03-01

Author

ARINC, E
SEN, A

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

147
views

0
downloads

Gilthead seabreams were exposed to benzocaine, 4-aminobenzoic acid ethyl ester, 57 mg/l in sea water for 3 min, daily, for 2 or 3 consecutive days. The fish were killed 20 hr after the last treatment. Benzocaine treatment for 2 or 3 days resulted in 57% and 67% inhibition of liver microsomal aniline 4-hydroxylase and ethylmorphine N-demethylase activities, respectively. The total cytochrome P450 content of fish liver microsomes was unaltered following the 2-day benzocaine treatment. However, additional 3 min benzocaine treatment on day 3 reduced cytochrome P450 level by 50%. Benzocaine produced type II difference spectra with rabbit liver microsomes. Difference spectra of fish liver microsomes elicited by benzocaine were complex. The position of peak and intensity were greatly influenced by the concentration of benzocaine.

Subject Keywords

Immunology, Pharmacology

URI

https://hdl.handle.net/11511/66110

Journal

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY

DOI

https://doi.org/10.1016/1367-8280(94)90068-x

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

COMPARATIVE-STUDIES OF SHEEP LUNG AND LIVER NITROFURANTOIN REDUCTASE İşcan, Mesude (Elsevier BV, 1993-10-01) 1. Nitrofurantoin reductase which catalyzes the bioactivation of nitrofurantoin was purified to electrophoretic homogenity from sheep liver and lung microsomes, with a yield of 15% and 35%, respectively. The specific activity of both reductases was found to be similar (140 nmol/min/mg protein). 2. The effects of nitrofurantoin and NADPH concentrations, pH, ionic strength, amount of enzyme and reaction period, on the enzyme activity were studied and the optimum conditions for maximum activity of purified fi...
EFFECTS OF INVIVO BENZENE TREATMENT ON CYTOCHROME-P450 AND MIXED-FUNCTION OXIDASE ACTIVITIES OF GILTHEAD SEABREAM (SPARUS-AURATA) LIVER-MICROSOMES ARINC, E; SEN, A (Elsevier BV, 1993-01-01) 1. Treatment of gilthead seabream (Sparus aurata) with benzene 0.6 ml/kg/day, s.c., for four consecutive days resulted in a marked 78% and 73% decrease in total cytochrome P450 content and 7-ethoxyresorufin O-deethylase activity of liver microsomes, respectively.
ANALYSIS OF PLASMIDS MEDIATING ANT (2'') AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE Kocabıyık, Semra (Elsevier BV, 1989-07-01) The genetics of ANT(2″) and AAC(3)-II mediated gentamicin resistance in Gram-negative bacteria obtained from Hacettepe University Hospital in Turkey, was studied. The plasmid DNA analyzed by agarose gel electrophoresis revealed a heterogeneous plasmid population. Conjugal transfer appeared to be an efficient mechanism for the dissemination of resistance to gentamicin/tobramycin/kanamycin/streptomycin/chloramphenicol/cephalothin, which was the common resistance pattern in the population. Plasmids of 46, 60, ...
Inhibition of the TIRAP-c-Jun interaction as a therapeutic strategy for AP1-mediated inflammatory responses Srivastava, Mansi; Saqib, Uzma; Banerjee, Sreeparna; Wary, Kishore; Kizil, Burak; Muthu, Kannan; Baig, Mirza S. (Elsevier BV, 2019-06-01) Bacterial endotoxin-induced sepsis causes 30-40% of the deaths in the intensive care unit (ICU) globally, for which there is no pharmacotherapy. Lipopolysaccharide (LPS), a bacterial endotoxin, stimulates the Toll-like receptor (TLR)-4 signalling pathways to upregulate the expression of various inflammatory mediators. Here, we show that the TIRAP and c-Jun protein signalling complex forms in macrophages in response to LPS stimulation, which increases the AP1 transcriptional activity, thereby amplifying the ...
Further immunochemical and biocatalytic characterization of CYP1A1 from feral leaping mullet liver (Liza saliens) microsomes Sen, A; Arinc, E (Elsevier BV, 2000-07-01) CYP1A is known to play important roles in the metabolism, detoxification and bioactivation of carcinogens and other xenobiotics in animals including fish. In our laboratory, CYP1A1 was obtained in a highly purified form with a specific content of 15-17 nmol P450 per mg protein from liver microsomes of feral fish, leaping mullet (Liza saliens). Purified mullet CYP1A1 showed a very high substrate specificities for 7-ethoxyresorufin and 7-methoxyresorufin in a reconstituted system containing purified fish P450...

Citation Formats

E. ARINC and A. SEN, “IN-VIVO EFFECTS OF THE ANESTHETIC, BENZOCAINE, ON LIVER MICROSOMAL CYTOCHROME-P450 AND MIXED-FUNCTION OXIDASE ACTIVITIES OF GILTHEAD SEABREAM (SPARUS-AURATA),” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, pp. 399–404, 1994, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66110.