Molecular detection of Nosema ceranae and N. apis from Turkish honey bees

Whitaker, Justin
Szalanski, Allen L.
Kence, Meral
Polymerase chain reaction specific for the rDNA marker for Nosema ceranae and Nosema apis was conducted on 84 Apis mellifera samples collected from 20 provinces in Turkey. N. ceranae was detected from three samples from the provinces of Artvin, Hatay, and Mugla. N. apis was detected in samples from the provinces of Sivas, Izmir, Bitlis and Gaziantep. All of the positive samples were from honey bees belonging to the 'C' lineage of A. mellifera. DNA sequencing analysis of the N. ceranae samples revealed that there was no intraspecific variation in the 208 bp of the 16S SSU of N. ceranae from Turkey. A TCS analysis revealed that the 16S SSU genotype from Turkey is identical to N. ceranae DNA sequences from Europe, Australia, and the United States. TCS analysis also revealed that this genotype is the basal ancestral genotype among six N. ceranae genotypes. This is the first study to confirm that N. ceranae is present in honey bees from Turkey.


Morphometric and genetic variability of honey bee (Apis mellifera L.) populations from northern Iran
Kence, Meral; Farhoud, Houmen Jabbari; Tunca, Rahsan Ivgin (Informa UK Limited, 2009-01-01)
Samples from seven honey bee populations; five Apis mellifera meda from Iran and two from Turkey, one belonging to A. m. meda and one to A. m. caucasica, were investigated using morphometric, mtDNA, and microsatellite analyses. Morphometric analysis revealed three distinct clusters. The first included all Iranian populations, whilst the second and third contained the meda and caucasica bees from Turkey. No variation was observed in the DraI restriction of COI-COII intergenic region in mitochondrial DNA, yie...
Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey
Kandemir, I; Kence, M; Kence, Aykut (Springer Science and Business Media LLC, 2000-05-01)
Six enzyme systems were studied to determine the genetic variability in honeybee populations in Turkey. Ten morphometric characters were also measured to determine the extent of morphometric variation. Out of six enzyme systems, four were found to be polymorphic with 16 allozymes. The average heterozygosity was calculated as 0.072 +/- 0.007. Morphometric and electrophoretic variables were equally effective in discriminating honeybee populations. European and Anatolian honeybees were separated on the first a...
Note: Pyrethroid resistance and possible involvement of glutathione S-transferases in Helicoverpa armigera from Turkey
Ugurlu, Sakine; Konus, Metin; Isgor, Belgin; İşcan, Mesude (Springer Science and Business Media LLC, 2007-01-01)
An introductory study was conducted to investigate the pyrethroid resistance of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) strains in Turkey, collected from cotton fields in the Adana and Antalya provinces, through two different synthetic pyrethroid insecticides: lambda-cyhalothrin and esfenvalerate. In addition, the roles of glutathione S-transferases (GSTs) in this resistance mechanism were analyzed. It was found that whereas resistance ratios for lambda-cyhalothrin (LD50 levels) were 3- and 9...
Standard survey methods for estimating colony losses and explanatory risk factors in Apis mellifera
van der Zee, Romee; Gray, Alison; Holzmann, Celine; Pisa, Lennard; Brodschneider, Robert; Chlebo, Robert; Coffey, Mary F.; Kence, Aykut; Kristiansen, Preben; Mutinelli, Franco; Bach Kim Nguyen, Bach Kim Nguyen; Noureddine, Adjlane; Peterson, Magnus; Soroker, Victoria; Topolska, Grazyna; Vejsnaes, Flemming; Wilkins, Selwyn (Informa UK Limited, 2013-01-01)
This chapter addresses survey methodology and questionnaire design for the collection of data pertaining to estimation of honey bee colony loss rates and identification of risk factors for colony loss. Sources of error in surveys are described. Advantages and disadvantages of different random and non-random sampling strategies and different modes of data collection are presented to enable the researcher to make an informed choice. We discuss survey and questionnaire methodology in some detail, for the purpo...
A comparative genetic analysis of the subterranean termite genus Reticulitermes (Isoptera : Rhinotermitidae)
Austin, JW; Szalanski, AL; Uva, P; Bagneres, AG; Kence, Aykut (Oxford University Press (OUP), 2002-11-01)
DNA sequencing analysis of the mitochondrial DNA cytochrome oxidase H (COII) region was used to examine genetic variation in the termite genus Reticulitermes Holmgren. We examined 21 species and subspecies from three continents. Sequencing of a 677-bp region of a 780-bp amplicon from 41 individuals and from 17 sequences obtained from GenBank revealed 221 polymorphic sites within the genus. Tajima-Nei distances from species ranged from 0.9 to 12.7%, and parsimony and maximum likelihood analysis revealed seve...
Citation Formats
J. Whitaker, A. L. Szalanski, and M. Kence, “Molecular detection of Nosema ceranae and N. apis from Turkish honey bees,” APIDOLOGIE, pp. 174–180, 2011, Accessed: 00, 2020. [Online]. Available: