Aromatase and C/EBP expression after global ischemia/reperfusion injury in rat hippocampus

Date

2009-08-15

Author

Kelicen, P.
Burul-Bozkurt, N.
Cincioglu, M.
Dagdeviren, M.
Pekiner, C.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

229
views

0
downloads

Background and aims: Global ischemia after cardiac arrest, intraoperative hypoxia/hypotension, or hemorrhagic shock is one of the causes of brain injury, resulting in severe neurological and neurobehavioral deficit. Since neurodegeneration can be protected from by local aromatase expression and estrogen synthesis can be neuroprotective in the ischemia/reperfusion process, aromatase may be a potential target to study reperfusion injury after brain ischemia. We investigated the expression of aromatase and C/EBP by using Western blotting in rat hippocampus after transient global ischemia + hypotension (CAO + jvht). Methods: Transient two vessel occlusion global ischemia plus hypotension was produced. Briefly, animals were anesthetized and body temperature maintained within the normal range (37.0–37.5 °C), throughout the experiment. The femoral artery was exposed and catheterized to allow continuous recording of arterial blood. Bilateral common carotid arteries were temporarily occluded (CAO), and blood was gradually withdrawn from the jugular vein to reduce the mean arterial blood pressure (MABP) to 35–45 mm Hg (ht). After 10 min of ischemia, the withdrawn warmed shed blood was reinfused to restore normotension and animals monitored during 3 h. Rats were decapitated, hippocampi were removed and homogenized and then immunoblotting was applied. Results: Protein expression of aromatase and C/EBP was observed after 10 min, 3 h and 24 h reperfusion period at the same level in both control and damaged tissues. These protein expressions peaked at 1 week and decayed at 2 weeks in damaged hippocampi after global ischemia-hypotension. Immunoblot analysis demonstrated that the highest aromatase and C/EBP expression appeared in the damaged (CAO + jvht) hippocampi after 1 week, and gradually reduced after 2–10 weeks. Conclusion: Our findings indicate aromatase and C/EBP expression result from ischemic as well as chronic neurodegenerative processes. ⁎This work is supported by H.U. project number: 02 02 301 009, approved by the ethical committee (2002/63-5).

Subject Keywords

Neurology, Clinical Neurology

URI

https://hdl.handle.net/11511/68163

DOI

https://doi.org/10.1016/j.jns.2009.02.040

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

Suggestions

OpenMETU
Core

Association analysis of Glutathione S-transferase omega-1 and omega-2 genetic polymorphisms and ischemic stroke risk in a Turkish population Bilgin, Esra; Can Demirdöğen, Birsen; Ozcelik, Aysun Turkanoglu; Demirkaya, Seref; Adalı, Orhan (Informa UK Limited, 2019-01-01) Objectives: Oxidative stress is a known risk factor for the pathogenesis of atherosclerosis, the main cause of ischemic stroke. Glutathione S-transferase (GST) omega-1 and omega-2, members of phase II enzymes, play a role in the antioxidant system. The single nucleotide polymorphisms (SNPs), C419A and A424G in GST omega genes can cause a decrease in enzyme activity. The aim of this study was to investigate the possible association between these polymorphisms and ischemic stroke risk in a Turkish population.
Association analysis of GSTT1, GSTM1 genotype polymorphisms and serum total GST activity with ischemic stroke risk Turkanoglu, Aysun; Demirdogen, Birsen Can; Demirkaya, Seref; Bek, Semai; Adalı, Orhan (Springer Science and Business Media LLC, 2010-12-01) Oxidative stress plays a major role in pathogenesis of atherosclerosis which is responsible for stroke. Glutathione S-transferases (GSTs) detoxify metabolites produced by oxidative stress within the cell and protect the cells against injury. This study aimed to investigate the association of polymorphisms in GSTT1, GSTM1 genes and GST activity with ischemic stroke risk. Patients had almost the same GST activity as that of controls. No significant differences were found between patients and controls in terms...
Genetic polymorphisms of vitamin D3 metabolizing CYP24A1 and CYP2R1 enzymes in Turkish patients with ischemic stroke ÖZÇELİK, AYSUN; ÖNER, TUĞÇE; Demirdogen, Birsen Can; BEK, VEDAT SEMAİ; DEMİRKAYA, ŞEREF; Adalı, Orhan (Informa UK Limited, 2018-01-01) Objective: Vitamin D deficiency is known as an important risk factor in pathogenesis of atherosclerosis, which contributes to stroke development. Genetic variations including single nucleotide polymorphisms (SNPs) in enzymes involved in vitamin D metabolism can affect susceptibility to the development of stroke. Therefore, the objective of this study was to investigate the association between polymorphisms of vitamin D metabolizing enzymes (rs927650 SNP in CYP24A1, and rs10741657 SNP in CYP2R1 genes,) and i...
Association analysis of glutathione s-transferase omega 1 and omega 2 genetic polymorphisms and ischemic stroke risk in Turkish population Bilgin, Esra; Adalı, Orhan; Department of Biochemistry (2014) Stroke is defined as the acute neurological cerebrovascular disease based on interruptions to blood flow in the brain. These interruptions are caused by loss of blood supply due to vessel bursts or vessel blocked by clotting. Atherosclerosis, a main cause of stroke, is blockage of endothelium layer of arteries and losing the flexibility of tissue. The oxidative stress is known as a risk factor for atherosclerosis. The increased free radicals such as reactive oxygen species (ROS) and decreased antioxidant le...
Epileptic seizures induce structural and functional alterations on brain tissue membranes Turker, Sevgi; Severcan, Mete; İLBAY, GÜL; Severcan, Feride (2014-12-01) Epilepsy is characterized by disruption of balance between cerebral excitation and inhibition, leading to recurrent and unprovoked convulsions. Studies are still underway to understand mechanisms lying epileptic seizures with the aim of improving treatment strategies. In this context, the research on brain tissue membranes gains importance for generation of epileptic activities. In order to provide additional information for this field, we have investigated the effects of pentylenetetrazol-induced and audio...

Citation Formats

P. Kelicen, N. Burul-Bozkurt, M. Cincioglu, M. Dagdeviren, and C. Pekiner, “Aromatase and C/EBP expression after global ischemia/reperfusion injury in rat hippocampus,” 2009, vol. 283, p. 248, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68163.