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Development of electrochemical biosensors with various types of zeolites
Date
2019-07-01
Author
Soldatkina, O. V.
Kucherenko, I. S.
Soldatkin, O. O.
Pyeshkova, V. M.
Dudchenko, O. Y.
Kurc, B. Akata
Dzyadevych, S. V.
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In the work, different types of zeolites were used for the development of enzyme-based electrochemical biosensors. Zeolites were added to the biorecognition elements of the biosensors and served as additional components of the biomembranes or adsorbents for enzymes. Three types of biosensors (conductometric, amperometric and potentiometric) were studied. The developed biosensors were compared with the similar biosensors without zeolites. The biosensors contained the following enzymes: urease, glucose oxidase, glutamate oxidase, and acetylcholinesterase and were intended for the detection of urea, glucose, glutamate, and acetylcholine, respectively. Construction of the biosensors using the adsorption of enzymes on zeolites has several advantages: simplicity, good reproducibility, quickness, absence of toxic compounds. These benefits are particularly important for the standardization and further mass production of the biosensors. Furthermore, a biosensor for the sucrose determination contained a three-enzyme system (invertase/mutatorase/glucose oxidase), immobilized by a combination of adsorption on silicalite and cross-linking via glutaraldehyde; such combined immobilization demonstrated better results as compared with adsorption or cross-linking separately. The analysis of urea and sucrose concentrations in the real samples was carried out. The results, obtained with biosensors, had high correlation with the results of traditional analytical methods, thus the developed biosensors are promising for practical applications.
Subject Keywords
Electrochemical biosensor
,
Zeolite
,
Silicalite
,
Enzyme
,
Adsorption
URI
https://hdl.handle.net/11511/68452
Journal
APPLIED NANOSCIENCE
DOI
https://doi.org/10.1007/s13204-018-0725-9
Collections
Graduate School of Natural and Applied Sciences, Article