Development of electrochemical biosensors with various types of zeolites

2019-07-01
Soldatkina, O. V.
Kucherenko, I. S.
Soldatkin, O. O.
Pyeshkova, V. M.
Dudchenko, O. Y.
Kurc, B. Akata
Dzyadevych, S. V.
In the work, different types of zeolites were used for the development of enzyme-based electrochemical biosensors. Zeolites were added to the biorecognition elements of the biosensors and served as additional components of the biomembranes or adsorbents for enzymes. Three types of biosensors (conductometric, amperometric and potentiometric) were studied. The developed biosensors were compared with the similar biosensors without zeolites. The biosensors contained the following enzymes: urease, glucose oxidase, glutamate oxidase, and acetylcholinesterase and were intended for the detection of urea, glucose, glutamate, and acetylcholine, respectively. Construction of the biosensors using the adsorption of enzymes on zeolites has several advantages: simplicity, good reproducibility, quickness, absence of toxic compounds. These benefits are particularly important for the standardization and further mass production of the biosensors. Furthermore, a biosensor for the sucrose determination contained a three-enzyme system (invertase/mutatorase/glucose oxidase), immobilized by a combination of adsorption on silicalite and cross-linking via glutaraldehyde; such combined immobilization demonstrated better results as compared with adsorption or cross-linking separately. The analysis of urea and sucrose concentrations in the real samples was carried out. The results, obtained with biosensors, had high correlation with the results of traditional analytical methods, thus the developed biosensors are promising for practical applications.
APPLIED NANOSCIENCE

Suggestions

Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites
Marchenko, Svitlana; Piliponskiy, Ihor I.; Mamchur, Olha O.; Soldatkin, Oleksandr O.; Kucherenko, Ivan S.; Kasap, Berna Ozansoy; Akata Kurç, Burcu; Dzyadevych, Sergei; Soldatkin, Alexei P. (2016-08-27)
This work is dedicated to the development of creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and creatinine deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was app...
Design of a biosensor based on 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H pyrrole
Tuncagil, Sevinc; Varis, Serhat; Toppare, Levent Kamil (2010-07-01)
Immobilization of polyphenol oxidase (tyrosinase, E.C. 1.14.18.1) was achieved on a copolymer of 1-(4-nitrophenyl)-2,5-di(2-thieny1)-1H-pyrrole [SNS(NO2)] with pyrrole USNS(NO2)]/PPy) via electrochemical polymerization. Two different substrates; catechol and L-tyrosine were used for the characterization of biosensor. The kinetic parameters of the biosensor, maximum reaction rate of the enzyme (V-max) and Michaelis-Menten constant (K-m) were determined for two different substrates. V-max was found as 0.02 mu...
Synthesis of CuO nanostructures on zeolite-Y and investigation of their CO2 adsorption properties
Boruban, Cansu; Nalbant Esentürk, Emren (2017-10-01)
Copper(II) oxide (CuO) nanoparticles (NPs) in two different morphologies, spiky and spherical, were synthesized on zeolite-Y by a modified impregnation method, and their CO2 adsorbing capabilities were investigated under standard conditions (1 atm and 298 K). The properties and CO2 adsorption performances of the hybrid systems were characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray, X-ray diffraction, X-ray photoelectron spectroscopy, atomic absorption s...
Development of antibacterial powder coatings using single and binary ion-exchanged zeolite A prepared from local kaolin
İşler Binay, Melda; KİRDECİLER, SALİH KAAN; Akata Kurç, Burcu (2019-12-01)
Antibacterial electrostatic powder paint coating with intended esthetic criteria was specially formulated for industrial applications using single (Ag+) and binary exchanged (Ag+ and Zn2+) zeolite A, which was prepared from local kaolin resources. Optimization studies on reaction parameters were conducted to obtain the most crystalline zeolite A from kaolin. The Ag+ and Zn2+ exchanged zeolite A samples prepared from kaolin were introduced as filler into the paint matrix during the electrostatic powder coati...
Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars
Dudchenko, Oleksandr Ye; Pyeshkova, Viktoriya M.; Soldatkin, Oleksandr O.; Akata Kurç, Burcu; Kasap, Berna O.; Soldatkin, Alexey P.; Dzyadevych, Sergei V. (2016-02-03)
The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four-to fivefold higher sensitivity than GOx-GA biosensor. It was concluded that silicalite together with GA sufficiently enhances enzyme ...
Citation Formats
O. V. Soldatkina et al., “Development of electrochemical biosensors with various types of zeolites,” APPLIED NANOSCIENCE, pp. 737–747, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68452.