Effects of maternal metabolic disorders on offspring antimicrobial peptides expression: change in antimicrobial defense system

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2020-9
Teker, Hikmet Taner
Birth is a traumatic and stressful event for all living organisms. Neonate meets a world full of microbes and has to adapt the new external environment. Throughout all developmental periods and adulthood stages, balanced microbiota crucial for the health of offspring. Antimicrobial peptides (AMP) have enormous impacts on the establishment of the microbiome and determining the specific composition of the bacterial community as well as its spatial organization. This thesis investigated the effects of maternal metabolic disorders on antimicrobial protein (AMP) expression, which is crucial for establishment of gastrointestinal system (GIS) probiotic composition and mucin expression on offspring intestinal cells. As Using whole-genome microarray analysis, changes in AMP expressions were detected and also ceratin gene expressions were validated by RT-PCR. Besides, changes in probiotic bacterial species populations were determined in those regions. Our results might reflect how maternal metabolic disorders fluctuate offspring mainly mucosal immunology parameters for the future microbiome establishment. Finally, tight junction proteins of intestinal cells were investigated to understand the pathogenic bacterial translocations that might be essential for chronic inflammation and obesity tendency of offspring.

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Citation Formats
H. T. Teker, “Effects of maternal metabolic disorders on offspring antimicrobial peptides expression: change in antimicrobial defense system,” Ph.D. - Doctoral Program, Middle East Technical University, 2020.