Effects of maternal metabolic disorders on offspring antimicrobial peptides expression: change in antimicrobial defense system

Teker, Hikmet Taner
Birth is a traumatic and stressful event for all living organisms. Neonate meets a world full of microbes and has to adapt the new external environment. Throughout all developmental periods and adulthood stages, balanced microbiota crucial for the health of offspring. Antimicrobial peptides (AMP) have enormous impacts on the establishment of the microbiome and determining the specific composition of the bacterial community as well as its spatial organization. This thesis investigated the effects of maternal metabolic disorders on antimicrobial protein (AMP) expression, which is crucial for establishment of gastrointestinal system (GIS) probiotic composition and mucin expression on offspring intestinal cells. As Using whole-genome microarray analysis, changes in AMP expressions were detected and also ceratin gene expressions were validated by RT-PCR. Besides, changes in probiotic bacterial species populations were determined in those regions. Our results might reflect how maternal metabolic disorders fluctuate offspring mainly mucosal immunology parameters for the future microbiome establishment. Finally, tight junction proteins of intestinal cells were investigated to understand the pathogenic bacterial translocations that might be essential for chronic inflammation and obesity tendency of offspring.


Effects of fetal alcohol and maternal intubation stress on the expression of proteins controlling postnatal development of male rat hippocampus
ELİBOL, BİRSEN; BEKER, MERVE; Jakubowska-Dogru, Ewa; KILIÇ, ÜLKAN (Informa UK Limited, 2020-09-01)
Background Developing brains can partially get over prenatal alcohol exposure-related detrimental conditions by activating some mechanisms involved in survival. Objectives This study aimed to shed light on the molecular correlates of compensatory mechanisms by examining temporal profiles in the expression of proteins controlling postnatal development in the rat hippocampus prenatally exposed to intubation stress/ethanol. Methods Male pups were randomly assigned to age subgroups (n = 21/age) which were sacri...
Predictors of pre- and postoperative anxiety in emergency surgery patients
Karancı, Ayşe Nuray (2003-10-01)
Objective: Emergency surgery is a highly stressful life event. Patients experience distressing anxiety both before and after surgery and this anxiety affects their recovery process. The aim of the present study is to examine variables related to pre- and postoperative anxiety in emergency surgery patients. Methods: The sample consisted of 146 emergency surgery patients who had abdominal surgery in an emergency surgery clinic. The research instruments were administered before and after the surgery. State Anx...
Shared signatures of social stress and aging in peripheral blood mononuclear cell gene expression profiles
Snyder-Mackler, Noah; Somel, Mehmet; Tung, Jenny (Wiley, 2014-10-01)
Chronic social stress is a predictor of both aging-related disease and mortality risk. Hence, chronic stress has been hypothesized to directly exacerbate the process of physiological aging. Here, we evaluated this hypothesis at the level of gene regulation. We compared two data sets of genome-wide gene expression levels in peripheral blood mononuclear cells (PBMCs): one that captured aging effects and another that focused on chronic social stress. Overall, we found that the direction, although not necessari...
Effects of human granulocyte-colony stimulating factor on fracture healing in rats
Bozlar, M; Aslan, B; Kalaci, A; Baktiroglu, L; Yanat, AN; Tasci, A (2005-08-01)
Objective: Granulocyte colony stimulation factor (G-CSF) is generally used to prevent and cure the neutropenia associated with chemotherapy and bone marrow transplantation. In addition to its effects on neutrophil function, G-CSF was found to have the characteristic of modulating the cytokines in the inflammatory response. Then, the question to answer is whether it has any effect on fracture healing and to what extent? In this study, we test the effects of G-CSF on the healing of tibia fracture in a rat model.
Activation of survival pathways in nutrient restricted colorectal cancer cells
Oral, Göksu; Banerjee, Sreeparna; Department of Biology (2023-1-9)
Limited nutrient availability in the tumor microenvironment can cause metabolic rewiring of cancer cells, resulting in the activation of various stress response pathways such as autophagy for survival. Our study showed for the first time that incubation of LoVo cells with a nutrient restriction medium containing low glucose, glutamine, and serum for 48 h resulted in the concurrent activation of two antagonistic proteins: the AMP Kinase pathway (AMPK) which is phosphorylated in response to low energy and act...
Citation Formats
H. T. Teker, “Effects of maternal metabolic disorders on offspring antimicrobial peptides expression: change in antimicrobial defense system,” Ph.D. - Doctoral Program, Middle East Technical University, 2020.