Gene expression profiling of Thermoplasma volcanium under extreme stress conditions

Date

2014-06-26

Author

Kocabıyık, Semra

Metadata

Show full item record

Item Usage Stats

149
views

0
downloads

Identifying the stress response mechanisms in Archaea mighthave potential applications considering the industrial importanceof their enzymes. In this study, as an extension of our researchon anti-stress mechanisms, we have carried out genome widetranscriptome analysis ofThermoplasma volcanium(grows opti-mally at pH 2.7 and 60◦C) under extreme stress (i.e., heat- shockat 68◦C, hydrogen peroxide, 0.03 mM H2O2and pH stress atpH 5.0) using AgilentCustom Gene Expression Microarray.The data was analyzed by using GeneSpring Ver 12.6 Software. With a pH shift from 2.7 to 5.0 most of the gene expressionwas repressed: of 47 genes differentially expressed by≥2-fold,66% were up-regulated and 34% were down-regulated. Among thenotably induced genes, some code for membrane proteins, sugarand amino acid transporters, cation transporter and antiporterswhich are crucial for maintaining cellular pH homeostasis. Oxida-tive stress mostly resulted in decreased gene expression; out of 452genes expressions of which changed≥2 fold, 75% were down regu-lated. Up regulation of ferrodoxins, oxidoreductases, thioredoxin,Fe-S oxidoreductases, aldo/keto reductases and dehydrogenases asa response to H2O2may be important for maintaining intracellularredox potentials and detoxifications. Temperature up-shift favoredthe expression of heat-shock proteins (including GroEL, sHSP, DnaK, DnaJ, GrpE) as well as genes related to carbohydrate metabolism(e.g., sugar permease).

URI

https://hdl.handle.net/11511/72494

DOI

https://doi.org/0.1016/j.nbt.2014.05.976

Conference Name

European Biotechnology Congress (22 - 26 Haziran 2014)

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum) Baloglu, Mehmet Cengiz; Oz, Mehmet Tufan; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2012-10-01) NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. C...
Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13) Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
Genes involved in mRNA surveillance are induced in Brachypodium distachyon under cadmium toxicity Aksoy, Emre; FİLİZ, ERTUĞRUL; ORMAN, ŞULE; ÇETİN, DURMUŞ; AKBUDAK, MEHMET AYDIN (2021-11-01) Background Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. Methods and results In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins,...
Bioactive Surface Design Based on Functional Composite Electrospun Nanofibers for Biomolecule Immobilization and Biosensor Applications Uzun, Sema Demirci; Kayaci, Fatma; UYAR, Tamer; TİMUR, SUNA; Toppare, Levent Kamil (2014-04-09) The combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with one-step electrospun nylon 6,6 nanofibers or 4% (w/w) multiwalled carbon nanotubes (MWCNTs) incorporating nylon 6,6 nanofibers (nylon 6,6/4MWCNT). High-resolution transmission electron microscopy study confirmed the successful incorporation of the MWCNTs in...
Molecular recognition of poly(A) by small ligands: an alternative method of analysis reveals nanomolar, cooperative and shape-selective binding Persil Çetinkol, Özgül (Oxford University Press (OUP), 2009-02-01) A few drug-like molecules have recently been found to bind poly(A) and induce a stable secondary structure (T(m)approximate to 60 degrees C), even though this RNA homopolymer is single-stranded in the absence of a ligand. Here, we report results from experiments specifically designed to explore the association of small molecules with poly(A). We demonstrate that coralyne, the first small molecule discovered to bind poly(dA), binds with unexpectedly high affinity (K(a) >10(7) M(-1)), and that the crescent sh...

Citation Formats

S. Kocabıyık, “Gene expression profiling of Thermoplasma volcanium under extreme stress conditions,” presented at the European Biotechnology Congress (22 - 26 Haziran 2014), Edinburgh, Saint Helena, 2014, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/72494.