Gene expression profiling of Thermoplasma volcanium under extreme stress conditions

Date

2014-06-26

Author

Kocabıyık, Semra

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Identifying the stress response mechanisms in Archaea mighthave potential applications considering the industrial importanceof their enzymes. In this study, as an extension of our researchon anti-stress mechanisms, we have carried out genome widetranscriptome analysis ofThermoplasma volcanium(grows opti-mally at pH 2.7 and 60◦C) under extreme stress (i.e., heat- shockat 68◦C, hydrogen peroxide, 0.03 mM H2O2and pH stress atpH 5.0) using AgilentCustom Gene Expression Microarray.The data was analyzed by using GeneSpring Ver 12.6 Software. With a pH shift from 2.7 to 5.0 most of the gene expressionwas repressed: of 47 genes differentially expressed by≥2-fold,66% were up-regulated and 34% were down-regulated. Among thenotably induced genes, some code for membrane proteins, sugarand amino acid transporters, cation transporter and antiporterswhich are crucial for maintaining cellular pH homeostasis. Oxida-tive stress mostly resulted in decreased gene expression; out of 452genes expressions of which changed≥2 fold, 75% were down regu-lated. Up regulation of ferrodoxins, oxidoreductases, thioredoxin,Fe-S oxidoreductases, aldo/keto reductases and dehydrogenases asa response to H2O2may be important for maintaining intracellularredox potentials and detoxifications. Temperature up-shift favoredthe expression of heat-shock proteins (including GroEL, sHSP, DnaK, DnaJ, GrpE) as well as genes related to carbohydrate metabolism(e.g., sugar permease).

URI

https://hdl.handle.net/11511/72494

DOI

https://doi.org/0.1016/j.nbt.2014.05.976

Conference Name

European Biotechnology Congress (22 - 26 Haziran 2014)

Collections

Department of Biology, Conference / Seminar

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Citation Formats

S. Kocabıyık, “Gene expression profiling of Thermoplasma volcanium under extreme stress conditions,” presented at the European Biotechnology Congress (22 - 26 Haziran 2014), Edinburgh, Saint Helena, 2014, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/72494.