Development of Non-Invasive and Biocompatible Thin Film Micro-Extraction Contact Lens Type Devices Compatible for In Vivo Metabolomics Investigations from Eye Surface

2019-09-01
Nalbant, Atakan Arda
Boyacı, Ezel
Solid phase microextraction (SPME), is a sampling and samp- le preparation technique in which the extraction of analytes is based on an equilibrium established between a sample and ext- ractive phase coated on a tip of a fiber. Thin film microextraction (TFME) which is a type of SPME provides high analytical sen- sitivity in environmental and biological samples and has gained interest in areas where no other technique can provide solutions. In this study, the aim was to develop biocompatible and flexib- le contact lens shaped thin film extractive membranes applicab- le to in vivo investigations with the ultimate goal of using them for small molecule sampling from eye surface. To make them suitable for sampling from the eye surface, the membranes are synthesized with polydimethylsiloxane (PDMS) using special templates. These extractive lenses are modified with various functional moieties to enhance their extraction coverage for polar and nonpolar analytes. Afterward, the membranes are tested for their extraction performances with in vitro test model. The small molecules (‹1500 Da) extracted from this model are separated by gas chromatography and liquid chromatography and detected by mass spectrometry (MS).
Citation Formats
A. A. Nalbant and E. Boyacı, “Development of Non-Invasive and Biocompatible Thin Film Micro-Extraction Contact Lens Type Devices Compatible for In Vivo Metabolomics Investigations from Eye Surface,” presented at the Euroanalysis 2019, (1 - 05 September 2019), İstanbul, Türkiye, 2019, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/76212.