Development of Non-Invasive and Biocompatible Thin Film Micro-Extraction Contact Lens Type Devices Compatible for In Vivo Metabolomics Investigations from Eye Surface

2019-09-01
Nalbant, Atakan Arda
Boyacı, Ezel
Solid phase microextraction (SPME), is a sampling and samp- le preparation technique in which the extraction of analytes is based on an equilibrium established between a sample and ext- ractive phase coated on a tip of a fiber. Thin film microextraction (TFME) which is a type of SPME provides high analytical sen- sitivity in environmental and biological samples and has gained interest in areas where no other technique can provide solutions. In this study, the aim was to develop biocompatible and flexib- le contact lens shaped thin film extractive membranes applicab- le to in vivo investigations with the ultimate goal of using them for small molecule sampling from eye surface. To make them suitable for sampling from the eye surface, the membranes are synthesized with polydimethylsiloxane (PDMS) using special templates. These extractive lenses are modified with various functional moieties to enhance their extraction coverage for polar and nonpolar analytes. Afterward, the membranes are tested for their extraction performances with in vitro test model. The small molecules (‹1500 Da) extracted from this model are separated by gas chromatography and liquid chromatography and detected by mass spectrometry (MS).
Euroanalysis 2019, (1 - 05 September 2019)

Suggestions

Novel conversion of thiols into disulfides, via S-nitrosothiol intermediates using trichloronitromethane
Demir, Ayhan Sıtkı; Igdir, AC; Mahasneh, AS (1999-10-15)
An efficient oxidative coupling of thiols to give disulfides via thionitrite (S-nitrosothiol) intermediate is described using trichloronitromethane as an efficient reagent in organic solvents and water. Cysteine and glutathione are converted into the corresponding disulfides in water in high yields.
Fungal deracemization of benzoin
Demir, AS; Hamamcı, Haluk; Sesenoglu, O; Neslihanoglu, R; Asikoglu, B; Capanoglu, D (2002-09-02)
An enzyme system of Rhizopus oryzae (ATCC 9363) catalyzes the inversion of the chirality of benzoin via a deracemization reaction and, depending on the pH of the medium, both enantiomers of benzoin are obtained in good yield and high ee starting from rac-benzoin.
Development of polycarbonate based zeolite 4A filled mixed matrix gas separation membranes
SEN, Deger; Kalıpçılar, Halil; Yılmaz, Levent (2007-10-15)
The incorporation of fillers, blending with multifunctional low molecular-weight additives and their combination are investigated as alternatives for modifying the permselective properties of polymeric gas separation membranes. For this purpose, pure polycarbonate (PC), PC/p-nitroaniline (pNA), PC/zeolite 4A and PC/pNA/zeolite 4A mixed matrix membranes were prepared by solvent-evaporation method using dichloromethane as solvent. Zeolite 4A was the filler and pNA was the low molecular-weight additive with mu...
Characterization of Metamaterials using a New Design and Measurement Technique for Microstrip Circuit Applications
Nesimoglu, Tayfun; Sabah, Cumali (2014-12-14)
Characterization of a metamaterial based on an S-Shaped resonator and a feeding transmission line using a new measurement technique is studied in this paper. First, the metamaterial is designed and simulated using conventional methods. Then, it is fabricated on microstrip and excited by using a microstrip transmission feedline. Measurements were carried out using a vector network analyzer by (SubMiniature version A) SMA coaxial terminations without needing any waveguide transmission lines. For both simulate...
Preparation, modification, and characterization of acrylic cements
Basgorenay, B; Ulubayram, K; Serbetci, K; Onurhan, E; Hasırcı, Nesrin (2006-03-15)
Acrylic cements with different compositions were prepared by mixing the solid part (composed of poly(methyl methacrylate), PMMA, and benzoyl peroxide, BPO) and the liquid part (composed of methyl methacrylate, MMA, and N,N-dimethyl-p-toluidine, DMPT), modified by addition of hydroxyapatite (HA) and ammonium nitrate (AN) and characterized by measuring thermal and mechanical properties. Three sets of samples were prepared. For B-group, the total amount of solid including HA was constant but the PMMA to HA rat...
Citation Formats
A. A. Nalbant and E. Boyacı, “Development of Non-Invasive and Biocompatible Thin Film Micro-Extraction Contact Lens Type Devices Compatible for In Vivo Metabolomics Investigations from Eye Surface,” presented at the Euroanalysis 2019, (1 - 05 September 2019), İstanbul, Türkiye, 2019, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/76212.