Doxorubicin-Loaded Dextran Coated Magnetic Nanoparticles Affect on Cytokines Gene Expression in Breast Cancer Cell Line

Date

2017-04-24

Author

Yalçın Azarkan, Serap
Mutlu, Pelin
Gündüz, Ufuk

Metadata

Show full item record

Item Usage Stats

173
views

0
downloads

Aims and Scopes: IL-6 and IL-8 are a well-known proinflammatory cytokines. IL-8 contributes to cancer progression through its potential functions as a mitogenic, motogenic and angiogenic factor. Interleukin (IL)-6 has important roles in the proliferation and metastasis[1,2]. In the present study, Dextran coated magnetic nanoparticles (Dox-Dex-MNPs) were prepared to obtain an effective targeted delivery system for Doxorubicin on breast cancer cell line. Materials and Methods: Dox-Dex-MNPs were synthesized and characterized by TEM, SEM, FTIR, VSM and TGA analyses. Drug loading efficiencies and release characteristics were investigated. On the other hand, drug-sensitive and drug-resistant MCF-7 cell lines were grown in RPMI 1640 medium supplemented with 10% FBS at 37oC and 5% CO2. Total RNA content was isolated by TRI Reagent according to the manufacturer‘s instructions. cDNA was synthesized from 1 ug of total RNA and random hexamer primers. The expression levels of IL-6 and IL-8 genes were shown using qRT-PCR. Results and Discussion: According to SEM, TEM, VSM and TGA results, the synthesized Dox-Dex-MNPs possess desired shape and size range (10-15 nm) as they can be internalized into the cell and also have superparamagnetic properties. FTIR analysis confirmed that Doxorubicin was successfully loaded on dextran coated MNPs. According to the gene expression results, IL-6 and IL8 significantly (6-fold and 9-fold, respectively) overexpressed in MCF-7/Dox cells with respect to the sensitive MCF-7 cell line. On the other hand, due to the application of free Doxorubicin to the MCF-7/Dox cells, both IL-6 and IL-8 genes 3 fold more overexpressed leading to 22- and 29-fold overexpression with respect to the sensitive MCF-7 cell line. Additionally, application of Dox-Dex-MNPs to MCF-7/Dox cell line decreased again the expression levels of these cytokines significantly.

Subject Keywords

IL-6, IL-8, Magnetic nanoparticles, Doxorubicin, Breast cancer

URI

https://hdl.handle.net/11511/78216
http://dna-day.com/dna17/wp-content/uploads/2017/05/IDDGC17-ABSTRACT-BOOK.pdf

Conference Name

International DNA Day And Genome Congress (24 - 28 Nisan 2017)

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

Suggestions

OpenMETU
Core

Drug resistant MCF-7 cells exhibit epithelial-mesenchymal transition gene expression pattern Iseri, Ozlem Darcansoy; Kars, Meltem Demirel; Arpaci, Fikret; Atalay, Can; Pak, Isin; Gündüz, Ufuk (2011-02-01) Purpose: Multidrug resistance is resistance to structurally unrelated anticancer agents. Large-scale expression analysis by using high-density oligonucleotide microarrays may provide information about new candidate genes contributing to MDR. This study demonstrates alterations in expression levels of several genes related to epithelial-mesenchymal transition (EMT) in paclitaxel, docetaxel, and doxorubicin resistant MCF-7 cells.
Convulsant agent pentylenetetrazol does not alter the structural and dynamical properties of dipalmitoylphosphatidylcholine model membranes Turker, Sevgi; Wassall, Stephen; Stillwell, William; Severcan, Feride (Elsevier BV, 2011-01-25) Pentylenetetrazol (PTZ) is an epileptogenic agent, which is widely used in the determination of epilepsy-induced alterations and in the assessment of anticonvulsant agents in epileptic studies. Even though PTZ is suggested to induce repetitive firing of nerve fibers and shorten the refractory, its mechanism of action is only partially understood. In the literature there are discrepancies for its action mechanism. While some studies stated that primary sires of PTZ are membrane proteins, some reports indicat...
Doxorubicin exhibits strong and selective association with VEGF Pu22 G-quadruplex Bilgen, Ecenaz; Persil Çetinkol, Özgül (Elsevier BV, 2020-12-01) Background: Vascular endothelial growth factor (VEGF), is upregulated in tumor cells and thus became a potential therapeutic target for anti-cancer drugs. Recent reports suggested the use of Doxorubicin (Dox) with VEGF-targeting siRNAs for an enhanced decrease in VEGF expression. Besides, VEGF-B gene therapy was found to suppress the cardiotoxicity effects of Dox. On the other hand, even though Dox is a commonly used anti-cancer agent, its mechanism of actions isn't completely mapped out. Herein, the intera...
Dielectrophoresis: Applications and future outlook in point of care Demircan, Yagmur; Ozgur, Ebru; Külah, Haluk (Wiley, 2013-04-01) Dielectrophoresis (DEP) is a label free, noninvasive, stand alone, rapid, and sensitive particle manipulation and characterization technique. Improvements in micro-electro-mechanical systems technology have enabled the biomedical applications of DEP over the past decades. By this way, integration of DEP into lab-on-a-chip systems has become achievable, creating a potential tool for point-of-care (POC) systems. DEP can be utilized in many different POC applications including early detection and prognosis of ...
Spectroscopic modifications of Pippard relations for ammonium halides Yurtseven, Hasan Hamit (1997-11-01) Spectroscopic modifications of the Pippard relations have been established for ammonium halides near their X-phase transitions. A linear relationship has been obtained between the specific heat and the frequency shifts using our observed Raman frequencies of the nu(5)TO(134 cm(-1)) and nu(5)LO(177 cm(-1)) lattice modes of NH4Br near its first-order phase transition (P = 0). Similar linear relationships have also been obtained using our observed Raman frequencies of the nu(5)TO(174 cm(-1)) lattice mode and t...

Citation Formats

S. Yalçın Azarkan, P. Mutlu, and U. Gündüz, “Doxorubicin-Loaded Dextran Coated Magnetic Nanoparticles Affect on Cytokines Gene Expression in Breast Cancer Cell Line,” presented at the International DNA Day And Genome Congress (24 - 28 Nisan 2017), Kırşehir, Türkiye, 2017, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/78216.