Doxorubicin-Loaded Dextran Coated Magnetic Nanoparticles Affect on Cytokines Gene Expression in Breast Cancer Cell Line

Date

2017-04-24

Author

Yalçın Azarkan, Serap
Mutlu, Pelin
Gündüz, Ufuk

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Aims and Scopes: IL-6 and IL-8 are a well-known proinflammatory cytokines. IL-8 contributes to cancer progression through its potential functions as a mitogenic, motogenic and angiogenic factor. Interleukin (IL)-6 has important roles in the proliferation and metastasis[1,2]. In the present study, Dextran coated magnetic nanoparticles (Dox-Dex-MNPs) were prepared to obtain an effective targeted delivery system for Doxorubicin on breast cancer cell line. Materials and Methods: Dox-Dex-MNPs were synthesized and characterized by TEM, SEM, FTIR, VSM and TGA analyses. Drug loading efficiencies and release characteristics were investigated. On the other hand, drug-sensitive and drug-resistant MCF-7 cell lines were grown in RPMI 1640 medium supplemented with 10% FBS at 37oC and 5% CO2. Total RNA content was isolated by TRI Reagent according to the manufacturer‘s instructions. cDNA was synthesized from 1 ug of total RNA and random hexamer primers. The expression levels of IL-6 and IL-8 genes were shown using qRT-PCR. Results and Discussion: According to SEM, TEM, VSM and TGA results, the synthesized Dox-Dex-MNPs possess desired shape and size range (10-15 nm) as they can be internalized into the cell and also have superparamagnetic properties. FTIR analysis confirmed that Doxorubicin was successfully loaded on dextran coated MNPs. According to the gene expression results, IL-6 and IL8 significantly (6-fold and 9-fold, respectively) overexpressed in MCF-7/Dox cells with respect to the sensitive MCF-7 cell line. On the other hand, due to the application of free Doxorubicin to the MCF-7/Dox cells, both IL-6 and IL-8 genes 3 fold more overexpressed leading to 22- and 29-fold overexpression with respect to the sensitive MCF-7 cell line. Additionally, application of Dox-Dex-MNPs to MCF-7/Dox cell line decreased again the expression levels of these cytokines significantly.

Subject Keywords

IL-6, IL-8, Magnetic nanoparticles, Doxorubicin, Breast cancer

URI

https://hdl.handle.net/11511/78216
http://dna-day.com/dna17/wp-content/uploads/2017/05/IDDGC17-ABSTRACT-BOOK.pdf

Conference Name

International DNA Day And Genome Congress (24 - 28 Nisan 2017)

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

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Citation Formats

S. Yalçın Azarkan, P. Mutlu, and U. Gündüz, “Doxorubicin-Loaded Dextran Coated Magnetic Nanoparticles Affect on Cytokines Gene Expression in Breast Cancer Cell Line,” presented at the International DNA Day And Genome Congress (24 - 28 Nisan 2017), Kırşehir, Türkiye, 2017, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/78216.