Preparation of a Nano-patterned Polymer Replica for Reducing Catheter-associated Infections

Lıu, Lutıng
Ercan, Batur
Sun, Lınlın
Webster, Thomas
Nowadays, catheter-associated infections are the most serious and costly of all healthassociated infections. [1] Recent studies have suggested a sensitivity of cell and bacteria to nano-scale topographical properties as a potential tool for selectively increasing desirable cell functions while simultaneously decreasing competitive cell functions. [2] Here, we presented a simple and cheap method to prepare a nano-patterned polydimethylsiloxane (PDMS, a commonly used catheter material) replica by using highly ordered nanotubular anodized titanium (ATi) as the template. In vitro bacterial studies using Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus epidermidis were conducted to assess the effectiveness of the nano-PDMS at inhibiting bacterial growth. In addition, human fibroblast and endothelial cell assays were conducted to determine the influence of the nanopatterned structure on mammalian cell behavior as a measurement of toxicity. In this study, to elucidate the mechanisms of how surface topographies affect cell/ bacteria adhesion, the protein interactions with different surfaces were also investigated by using the bicinchoninic acid (BCA) protein assay.
Society For Biomaterials 2015 Annual Meeting and Exposition (15 - 18 Nisan 2015)


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Citation Formats
L. Lıu, B. Ercan, L. Sun, and T. Webster, “Preparation of a Nano-patterned Polymer Replica for Reducing Catheter-associated Infections,” presented at the Society For Biomaterials 2015 Annual Meeting and Exposition (15 - 18 Nisan 2015), Charlotte, North Carolina, USA, 2015, Accessed: 00, 2021. [Online]. Available: