The polymeric membranes for antimicrobial applications are in high demand nowadays, since the bacteria had developed the resistance against the antibiotics. The membrane technology could become an efficient tool to cope with the antibiotic resistant bacteria which are becoming an unevitable problem on the health and pocket of a common man. To address this issue, the current research is carried out to synthesize polymer with antibacterial properties. Norbornene and oxonorborne monomers with special modification to achieve antimicrobial properties through N-halamine functional group of hydantoin was synthesized in this thesis. The monomers were polymerized using Ring Opening Metathesis Polymerization (ROMP). The polymer norbornene dicarboximide hydantoin P(NDH) and polymer oxonorbornene dicarboximide hydantoin P(ONDH) were saturated to s-P(NDH) and s-P(ONDH) with p-toluenesulfonyl hydrazide. Polymer s-P(NDH) sucessfuly fabricated into membrane. While s-P(ONDH) could not be fabricated into membranes by itself, the s-P(ONDH) was blended with the polyether sulfone (PES). The s-P(NDH) was also blended with PES, along with PES modified cyanuric acid for comparisons. Hence four membranes were studied, one was homopolymer and three were the blend polymer membranes. The synthesized polymers and the blend membranes were characterized using NMR, IR, DSC, and TG/DTA respectively. The properties of membranes were measured: with SEM, pore sizes were determined: with contact angle measurements, hydrophobocity of surface was determined. Besides, tests for the pure water permeance PWP and BSA protein rejection were conducted. The membranes were studied for their antimicrobial properties against E. coli and S. aureus. The respective membranes were efficient enough to give 100% kill of both bacteria, providing the controlled release of active chlorine and restricted the biofilm formation at the surface of membrane.


Development and analysis of controlled release polymeric rods containing vancomycin
Tağıt, Oya; Hasırcı, Vasıf Nejat; Department of Biotechnology (2005)
Antibiotic use is a vital method for the treatment of most diseases involving bacterial infections. Unfortunately, in certain cases these agents are not effective in treatments against diseases for either some limitation in antibiotic usage because of the side effects or some distribution problems caused by physiological or pathological barriers in the body. Such problems are thought to be minimized by development of controlled release systems which involve implantation of antibiotic loaded polymeric system...
Backbone resonance assignments of a promiscuous aminoglycoside antibiotic resistance enzyme; the aminoglycoside phosphotransferase(3')-IIIa
Serpersu, Engin H.; Özen, Can; Norris, Adrianne L.; Steren, Carlos; Whittemore, Neil (2010-04-01)
The aminoglycoside phosphotransferase(3')-IIIa (APH) is a promiscuous enzyme and renders a large number of structurally diverse aminoglycoside antibiotics useless against infectious bacteria. A remarkable property of this similar to 31 kDa enzyme is in its unusual dynamic behavior in solution; the apo-form of the enzyme exchanges all of its backbone amide protons within 15 h of exposure to D (2) O while aminoglycoside-bound forms retain similar to 40% of the amide protons even after > 90 h of exposure. More...
Determination of colistin resistance in Escherichia coli isolates from foods in Turkey, 2011-2015
Güzel, Mustafa; Avşaroğlu, M Dilek; Soyer, Yeşim (2020-05-01)
Antimicrobial resistance of pathogenic microorganisms is an emerging public health concern. Intensive use of antibiotics in food animals might increase antimicrobial resistance in foodborne pathogens. Colistin is a last resort antibiotic for treatment of multidrug resistant (MDR) Gram negative pathogens. The recent antimicrobial resistance studies revealed a mobile antimicrobial resistance gene (mcr) that provides resistance to colistin. Furthermore, the gene has been found in different genera. Therefore, t...
Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity
Roy, Anupam; Bulut, Onur; Some, Sudip; Mandal, Amit Kumar; Yilmaz, M. Deniz (Royal Society of Chemistry (RSC), 2019-01-23)
Since discovery of the first antibiotic drug, penicillin, in 1928, a variety of antibiotic and antimicrobial agents have been developed and used for both human therapy and industrial applications. However, excess and uncontrolled use of antibiotic agents has caused a significant growth in the number of drug resistant pathogens. Novel therapeutic approaches replacing the inefficient antibiotics are in high demand to overcome increasing microbial multidrug resistance. In the recent years, ongoing research has...
Socio-economic factors, cultural values, national personality and antibiotics use: A cross-cultural study among European countries
Gaygisiz, Ummugulsum; Lajunen, Timo; Gaygısız Lajunen, Esma (2017-11-01)
There are considerable cross-national differences in public attitudes towards antibiotics use, use of prescribed antibiotics, and self-medication with antibiotics even within Europe. This study was aimed at investigating the relationships between socio-economic factors, cultural values, national personality characteristics and the antibiotic use in Europe. Data included scores from 27 European countries (14 countries for personality analysis). Correlations between socio-economic variables (Gross National In...
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