Dielectrophoretic Separation of K562 Human Leukemia Cells From Leukocytes by Isolated 3D Electrodes

Bahrieh, Garsha
Özgür, Ebru
Külah, Haluk
This paper presents the design, simulation, and fabrication of a MEMS based dielectrophoresis (DEP) platform for the separation and detection of the human leukemic cancer cells (K562) and its multidrug resistant (MDR) counterpart (K56/IMA-0.2 µM) from the human leukocyte mixture. The pro-posed DEP device, benefits from the 3D isolated electrodes inside a microfluidic channel. 3D elec-trodes (extruded 30 µm in z-direction) were isolated from the medium with thin parylene coating (~ 0.3 µm). In addition, the potentially-floating 3D elec-trodes were utilized to enhance the DEP separation efficiency, for the first time in the literature. These electrodes, elevates the performance of the device by extending the generated electric field gradient inside the DEP separation area. FEM electric field and incompressible Navier-Stoke analyses were utilized to study the separation efficiency of the proposed device. It is shown that under the optimized boundary conditions, the proposed device can achieve separa-tion of K562 human leukemia cells from the human leukocytes mixture, with 100% efficiency.
25th Micromechanics and Microsystems Europe workshop (MME 2014)


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Citation Formats
G. Bahrieh, E. Özgür, and H. Külah, “Dielectrophoretic Separation of K562 Human Leukemia Cells From Leukocytes by Isolated 3D Electrodes,” presented at the 25th Micromechanics and Microsystems Europe workshop (MME 2014), İstanbul, Turkey, 2014, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/82804.