intI1 Type Mobile Genetic Elements Co-selected Antibiotic-Resistant Genes in Untreated Hospital Wastewaters

Date

2021-01-01

Author

Kayali, Osman
İçgen, Bülent

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

146
views

0
downloads

Dissemination of antibiotic-resistant genes (ARGs) from hospital wastewaters (HWWs) is facilitated by the horizontal gene transfer (HGT) and involves association of ARGs with mobile genetic elements (MGEs). In our previous study, HWWs were found to have relatively high copy numbers of ARGs aadA, tetA, cmlA, sul1, and qnrS. In this study, therefore, the same HWWs were also monitored for 3 MGEs class 1 integron (intI1), insertion sequence common region 1 (ISCR1) and conjugative transposon Tn916/Tn1545 by using quantitative polymerase chain reaction. The gene intI1 with 7.4 x 10(2) average copy number/mL was found to be the most prevalent MGE and was up to two orders of magnitude higher than ISCR1 (5.5 x 10(0) average copy number/mL, p < 0.05) and Tn916/Tn1545 (2.3 x 10(0) average copy number/mL, p < 0.05) in all HWWs tested. Positive correlation between intI1 and the aadA, tetA, cmlA and sul1 genes indicated that the MGEs harbouring class1 integron most likely played major role in co-selecting all these ARGs together.

URI

https://hdl.handle.net/11511/88818

Journal

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

DOI

https://doi.org/10.1007/s00128-020-03098-z

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

APOBEC3B expression in drug resistant MCF-7 breast cancer cell lines Onguru, Onder; Yalcin, Serap; Rosemblit, Cinthia; Zhang, Paul J.; Kilic, Selim; Gündüz, Ufuk (2016-04-01) APOBEC3B belongs to a protein family of cytidine deaminases that can insert mutations in DNA and RNA as a result of their ability to deaminate cytidine to uridine. It has been shown that APOBEC3B-catalysed deamination provides a chronic source of DNA damage in breast cancers. We investigated APOBEC3B expression in four drug resistant breast cancer cell lines (Doxorubicin, Etoposide, Paclitaxel and Docetaxel resistant MCF-7 cell lines) using a novel RNA in situ hybridization technology (RNAscope) and compare...
Inference of Gene Regulatory Networks Via Multiple Data Sources and a Recommendation Method Ozsoy, Makbule Gulcin; Polat, Faruk; Alhajj, Reda (2015-11-12) Gene regulatory networks (GRNs) are composed of biological components, including genes, proteins and metabolites, and their interactions. In general, computational methods are used to infer the connections among these components. However, computational methods should take into account the general features of the GRNs, which are sparseness, scale-free topology, modularity and structure of the inferred networks. In this work, observing the common aspects between recommendation systems and GRNs, we decided to ...
ALCAM (Activated Leukocyte Cell Adhesion Molecule) Yavuz, Esra; ÖYKEN, MERVE; Erson Bensan, Ayşe Elif (2018-01-01) ALCAM (Activated Leukocyte Cell Adhesion Molecule), also known as CD166 (cluster of differentiation 166), is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain.
Building Dicer1 regulation network of mouse liver hepatocytes Ünsal, Şeyma; Beyge, Mahmut; Aydın Son, Yeşim (2012-06-29) RNA induced gene silencing complex (RISC) has a role in many cellular processes which includes regulation of gene expression, immune response, cell differentiation and embryonic development. Dicer protein is a key regulator of these processes. Dicer1 specifically has central role in maturation of RISC substrate RNA and RISC assembly in mouse. Here the results of a microarray study that investigates the molecular level changes in dicer1 knockout mouse liver hepatocytes is re-analysed and the differentially r...
Epitranscriptomics of ischemic heart disease-the IHD-EPITRAN study design and objectives Sikorski, Vilbert; et. al. (2021-06-02) Licensee MDPI, Basel, Switzerland.Epitranscriptomic modifications in RNA can dramatically alter the way our genetic code is deciphered. Cells utilize these modifications not only to maintain physiological processes, but also to respond to extracellular cues and various stressors. Most often, adenosine residues in RNA are targeted, and result in modifications including methylation and deamination. Such modified residues as N-6-methyl-adenosine (m6A) and inosine, respectively, have been associated with cardi...

Citation Formats

O. Kayali and B. İçgen, “intI1 Type Mobile Genetic Elements Co-selected Antibiotic-Resistant Genes in Untreated Hospital Wastewaters,” BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/88818.