Boron nitride/zinc doped hydroxyapatite/polycaprolactone composite scaffolds for bone tissue engineering

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2021-3

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Turhan, Emine Ayşe

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Bone diseases and disorders have been expected to increase mostly in time because of the aging, obesity and pysical activity problems. For this reason, bone tissue engineering has been focus point to design new biocompatible scaffolds and enhance bone tissue regeneration. Composite scaffolds composed of PCL, Zn doped hydoxyapatite (HA), and boron nitride nanofibers (BNNFs) were prepared by the rotary jet spinning for bone tissue engineering applications. Synthesis of BNNFs and Zn-HA was achieved by the freeze drying and the wet chemical methods, respectively and then verified by XRD, FTIR, SEM and ICP analyses. The composite scaffolds were characterized for morphological properties, bioactivity, biocompatibility and mechanical properties. The bioactivity of PCL, PCL-Zn HA, PCL-Zn HA-BNNF and PCL-BNNF scaffolds was tested in simulated body fluids (SBF) and PCL-Zn HA-BNNF group showed good bioactivity by forming higher amount of apatite layer. Furthermore, proliferation and osteogenic activity of cells in all groups were investigated by Saos-2 cells. The results showed that the highest proliferation and osteogenic activity of cells were obtained in PCL-Zn HA-BNNF group. Furthermore, compression test results revealed that compressive properties of PCL scaffolds were improved by the addition of both BNNF and Zn HA but tensile tests showed that addition of Zn HA or BNNF reduced the strength of PCL due to the agglomeration of particles. Our results showed that PCL-Zn HA-BNNF scaffolds holds promise for bone tissue engineering applications

Subject Keywords

Hydroxyapatite, Boron nitride nanofiber, PCL, Bone tissue engineering, Rotary jet spinning

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https://hdl.handle.net/11511/89903

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

E. A. Turhan, “Boron nitride/zinc doped hydroxyapatite/polycaprolactone composite scaffolds for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2021.