Triglyceride dependent differentiation of obesity in adipose tissues by FTIR spectroscopy coupled with chemometrics

Baloglu, Fatma Kucuk
Baloğlu, Onur
Heise, Sebastian
Brockmann, Gudrun
Severcan, Feride
The excess deposition of triglycerides in adipose tissue is the main reason of obesity and causes excess release of fatty acids to the circulatory system resulting in obesity and insulin resistance. Body mass index and waist circumference are not precise measure of obesity and obesity related metabolic diseases. Therefore, in the current study, it was aimed to propose triglyceride bands located at 1770-1720 cm(-1) spectral region as a more sensitive obesity related biomarker using the diagnostic potential of Fourier Transform Infrared (FTIR) spectroscopy in subcutaneous (SCAT) and visceral (VAT) adipose tissues.


Biophysical characterization and diagnosis of obesity from adipose tissue by fourier transform infrared spectroscopy and imaging
Küçük Baloğlu, Fatma; Severcan, Feride; Department of Biology (2017)
Obesity is a heterogeneous disorder originating from the enlargement of visceral (VAT) and subcutaneous (SCAT) adipose tissue mass in the body and this process usually results in disturbed glucose and lipid metabolism. The first part of this study aimed to characterize and compare VAT and SCAT with regard to biomolecular content and also investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1)...
Macromolecular characterization of apidose tissues in inbred obese mouse models
Şen, İlke; Severcan, Feride; Banerjee, Sreeparna; Department of Biology (2012)
Obesity is a metabolic disorder that results in elevated levels of free fatty acids and triglycerides in the blood circulation, which further leads to accumulation of lipids within various tissues. Like in other similar metabolic disorders, obesity is thought to be originated from structural and regulatory changes in macromolecular assemblies. This current study aims to investigate the effects of obesity on macromolecular alterations in order to characterize Berlin fat mouse inbred (BFMI) lines which arenew...
Lipid Profiles of Adipose and Muscle Tissues in Mouse Models of Juvenile Onset of Obesity Without High Fat Diet Induction: A Fourier Transform Infrared (FT-IR) Spectroscopic Study
Sen, Ilke; Bozkurt, Ozlem; Aras, Ebru; Heise, Sebastian; Brockmann, Gudrun Anni; Severcan, Feride (SAGE Publications, 2015-06-01)
The current study aims to determine lipid profiles in terms of the content and structure of skeletal muscle and adipose tissues to better understand the characteristics of juvenile-onset spontaneous obesity without high fat diet induction. For the purposes of this study, muscle (longissimus, quadriceps) and adipose (inguinal, gonadal) tissues of 10-week-old male DBA/2J and Berlin fat mouse inbred (BFMI) lines (BFMI856, BFMI860, BFMI861) fed with a standard breeding diet were used. Biomolecular structure and...
Diabetes induces compositional, structural and functional alterations on rat skeletal soleus muscle revealed by FTIR spectroscopy: a comparative study with EDL muscle
Bozkurt, Ozlem; Severcan, Mete; Severcan, Feride (2010-01-01)
Diabetes Mellitus (DM) is a metabolic disorder, characterized by abnormally high blood glucose levels due to decreased secretion or effectiveness in function of insulin. Having a role in carbohydrate and lipid metabolism, skeletal muscle is affected by the absence of insulin in diabetic conditions. This current study reports the application of Fourier transform infrared (FTIR) spectroscopy in the determination of macromolecular alterations in streptozotocin (STZ)-induced diabetic rat skeletal Soleus (SOL) m...
Investigation of Gender Effect on Obesity using a Model of Inbreed Obese Mouse Lines by Fourier Transform Infrared Imaging
Baloglu, Fatma Kucuk; Brockmann, Gudrun; Heise, Sebastian; Garip, Sebnem; Severcan, Feride (2015-01-27)
Lipid accumulation and storage of lipids in adipocytes during obesity cause structural and functional changes in adipose tissue conformation. The expansion of visceral (VAT) and subcutaneous (SCAT) adipose tissue mass in the body is the main reason of obesity and many times it results in disturbed lipid and glucose metabolism. Gender is an important factor for the research in obesity and other metabolic diseases because it leads to different fat distribution and the pathophysiology. This study aims to deter...
Citation Formats
F. K. Baloglu, O. Baloğlu, S. Heise, G. Brockmann, and F. Severcan, “Triglyceride dependent differentiation of obesity in adipose tissues by FTIR spectroscopy coupled with chemometrics,” JOURNAL OF BIOPHOTONICS, pp. 1345–1355, 2017, Accessed: 00, 2020. [Online]. Available: