Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering

Date

2021-06-01

Author

Alshemary, Ammar Z.
Bilgin, Saliha
Işık, Gülhan
Motameni, Ali
Tezcaner, Ayşen
Evis, Zafer

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

296
views

0
downloads

Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials. In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements. Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system. Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP. Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape. Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra. DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water. SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%). The prepared cements were characterized with XRD, SEM, FTIR and Thermogravimetric analysis (TGA). XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase. SEM results confirmed that addition of SA inhibited the growth of DCP particles. Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements. In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C. The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation. Amount of Ca2+ ions released in SBF increased with the addition of SA. DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods. In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs). Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively. All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group. Based on the outcomes, SA/DCP bone cements have a promising future to be utilized as bone filler.

Subject Keywords

Dicalcium phosphate cement, Sodium alginate, Setting time, Biomechanics, In vitro cell, Culture analysis

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85101975851&origin=inward
https://hdl.handle.net/11511/90789

Journal

Journal of the Mechanical Behavior of Biomedical Materials

DOI

https://doi.org/10.1016/j.jmbbm.2021.104439

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Stem Cell and Advanced Nano Bioceramic Interactions Kose, Sevil; Kankilic, Berna; Gizer, Merve; Dede, Eda Ciftci; Bayramlı, Erdal; KORKUSUZ, PETEK; KORKUSUZ, FEZA (2018-01-01) Bioceramics are type of biomaterials generally used for orthopaedic applications due to their similar structure with bone. Especially regarding to their osteoinductivity and osteoconductivity, they are used as biodegradable scaffolds for bone regeneration along with mesenchymal stem cells. Since chemical properties of bioceramics are important for regeneration of tissue, physical properties are also important for cell proliferation. In this respect, several different manufacturing methods are used for manuf...
Replacement of the Basic and Acidic Components of Magnesium Potassium Phosphate Cement with Calcium Sulphoaluminate Cement and Fly Ash Phosphate Salts Mazaheri Shadbad, Hossein; Erdoğan, Sinan Turhan; Akgül, Çağla; Department of Civil Engineering (2022-12-02) Magnesium phosphate cement (MPC) is an acid-base cement with applications as a construction material. This cement has properties superior to portland cement like high early strength. However, MPC has a short setting time, high heat of hydration, and poor water resistance. In addition, this cement uses dead-burned magnesium oxide, prepared at around 1500 ˚C, which consumes much energy, releases CO2, and increases cost. In this study, the two main components of magnesium potassium phosphate cement, dead-burne...
Dip coating of calcium hydroxyapatite on Ti-6Al-4V substrates Mavis, B; Tas, AC (2000-04-01) Ti-6Al-4V alloy is the most commonly used metallic material in the manufacture of orthopedic implants. The main inorganic phase of human bone is calcium hydroxyapatite (Ca-10(PO4)(6)-(OH)(2), HA). To achieve better biocompatibility with bone, metal implants made of Ti-6Al-4V are often coated with bioceramics. Dip-coating techniques scarcely are used to apply HA onto metallic implants. New dipping-solution recipes to be used for HA coatings are described in this work. Scanning electron microscopy and X-ray d...
Production of fly ash-magnesium phosphate cements Bilginer, Baki Aykut; Erdoğan, Sinan Turhan (null; 2019-05-02) Magnezyum fosfat çimentolu (özellikle magnezyum potasyum fosfat çimentolu [MKPC]) bağlayıcılar Portland çimentosuna kısmi alternatif olabilecek sistemlerdir. MKPC yaygın olarak kalsine MgO tozu ve potasyum dihidrojen fosfat tuzu kullanılarak hazırlanır. Sulu karışımda meydana gelen asit-baz tepkimesi çok hızlı gerçekleştiğinden, bir geciktirici (boraks) de eklenir. MKPC’nin çabuk priz alma, çok hızlı dayanım kazanma ve yüksek nihai dayanım gibi geleneksel çimentolu sistemlere üstün yan...
Biomimetic preparation of HA precursors at 37 degrees C in urea- and enzyme urease-containing synthetic body fluids Bayraktar, D; Tas, AC (1999-12-01) An important inorganic phase of synthetic bone applications, calcium hydroxyapatite (HA, Ca-10(PO4)(6)(OH)(2)), was prepared as a single-phase and sub-micron bioceramic powder. Carbonated HA precursors were synthesized from calcium nitrate tetrahydrate and diammonium hydrogen phosphate salts dissolved in "synthetic body fluid" (SBF) solutions, containing urea (H2NCONH2) and enzyme urease, under the biomimetic conditions of 37 degrees C and pH 7.4, by using a novel chemical precipitation technique.

Citation Formats

A. Z. Alshemary, S. Bilgin, G. Işık, A. Motameni, A. Tezcaner, and Z. Evis, “Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering,” Journal of the Mechanical Behavior of Biomedical Materials, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85101975851&origin=inward.