The synthesis and characterization of thioglycolic acid and thiourea capped fluorescent zinc sulfide nanoparticles

Download

Cemre Mertoglu-Master Thesis.pdf

Date

2021-6

Author

Mertoğlu, Cemre

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

438
views

211
downloads

The changes in mitochondrial bioenergetics due to DNA mutations lead to an increase in the significance of mitochondria imaging of cancer cells. Since fluorescent imaging provides high resolution, sensitivity, and selectivity to many targets in living cells, fluorescent nanoparticles can be used as imaging probes. Zinc is one of the essential elements in cells used for cell growth, division, and apoptosis. Therefore, zinc sulfide semiconductor nanoparticles have been attracted attention due to its characteristic optical, chemical, and physical properties as well as low toxicity. In this study, it was aimed to synthesize surface functionalized zinc sulfide semiconductor nanoparticles for mitochondria targeting and imaging of cancer cells. Thioglycolic acid and thiourea capped zinc sulfide nanoparticles were synthesized in an aqueous medium at room temperature. The synthesized zinc sulfide nanoparticles were coated with triphenylphosphonium cation, a targeting moiety, through carbodiimide crosslinking. The fluorescence emission peak of thioglycolic acid and thiourea capped zinc sulfide nanoparticles was at 648 nm. Triphenylphosphonium coated zinc sulfide nanoparticles showed an emission maximum at 650 nm. The morphological characterization of the surface functionalized zinc sulfide nanoparticles was performed by Transmission Electron Microscopy. Surface functionalization of triphenylphosphonium coated zinc sulfide nanoparticles was performed by heparin and glucose coating, and they were used for intracellular analysis. Intracellular analysis is started to be performed and in progress.

Subject Keywords

Zinc sulfide nanoparticles, Triphenylphosphonium, Fluorescence spectroscopy, Mitochondria imaging

URI

https://hdl.handle.net/11511/91340

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Highly-sensitive and fast detection of human telomeric G-Quadruplex DNA based on a hemin-conjugated fluorescent metal-organic framework platform Javan Kouzegaran, Vahid; Farhadi, Khalil; Forough, Mehrdad; Bahram, Morteza; Persil Çetinkol, Özgül (Elsevier BV, 2021-04-15) © 2021 Elsevier B.V.The formation of G-quadruplex (G4) structures in Human telomeric DNA (H-Telo) has been demonstrated to inhibit the activity of telomerase enzyme that is associated with the proliferation of many cancer cells. Accordingly, G-quadruplex structures have become one of the well-established targets in anticancer therapeutic strategies. And, the development of simple and selective detection platforms for G4 structures has become a significant focus of research in recent years. In this study, a ...
Role of Vibrational Spectroscopy in Stem Cell Research Aksoy, Ceren; Severcan, Feride (2012-01-01) Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering GÜVEN, BURCU; BOYACI, İSMAİL HAKKI; TAMER, UĞUR; Çalık, Pınar (Royal Society of Chemistry (RSC), 2012-01-01) In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activa...
Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group Mutlu, M.; Mutlu, Pelin; Azarkan, S.; Baylr, Ö.; Öcal, B.; Saylam, G.; KORKMAZ, MEHMET HAKAN (2021-03-23) Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Y...
A validated active contour method driven by parabolic arc model for detection and segmentation of mitochondria Tasel, Serdar F.; Mumcuoğlu, Ünal Erkan; Hassanpour, Reza Z.; Perkins, Guy (2016-06-01) Recent studies reveal that mitochondria take substantial responsibility in cellular functions that are closely related to aging diseases caused by degeneration of neurons. These studies emphasize that the membrane and crista morphology of a mitochondrion should receive attention in order to investigate the link between mitochondria] function and its physical structure. Electron microscope tomography (EMT) allows analysis of the inner structures of mitochondria by providing highly detailed visual data from l...

Citation Formats

C. Mertoğlu, “The synthesis and characterization of thioglycolic acid and thiourea capped fluorescent zinc sulfide nanoparticles,” M.S. - Master of Science, Middle East Technical University, 2021.