The synthesis and characterization of thioglycolic acid and thiourea capped fluorescent zinc sulfide nanoparticles

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Cemre Mertoglu-Master Thesis.pdf

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2021-6

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Mertoğlu, Cemre

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The changes in mitochondrial bioenergetics due to DNA mutations lead to an increase in the significance of mitochondria imaging of cancer cells. Since fluorescent imaging provides high resolution, sensitivity, and selectivity to many targets in living cells, fluorescent nanoparticles can be used as imaging probes. Zinc is one of the essential elements in cells used for cell growth, division, and apoptosis. Therefore, zinc sulfide semiconductor nanoparticles have been attracted attention due to its characteristic optical, chemical, and physical properties as well as low toxicity. In this study, it was aimed to synthesize surface functionalized zinc sulfide semiconductor nanoparticles for mitochondria targeting and imaging of cancer cells. Thioglycolic acid and thiourea capped zinc sulfide nanoparticles were synthesized in an aqueous medium at room temperature. The synthesized zinc sulfide nanoparticles were coated with triphenylphosphonium cation, a targeting moiety, through carbodiimide crosslinking. The fluorescence emission peak of thioglycolic acid and thiourea capped zinc sulfide nanoparticles was at 648 nm. Triphenylphosphonium coated zinc sulfide nanoparticles showed an emission maximum at 650 nm. The morphological characterization of the surface functionalized zinc sulfide nanoparticles was performed by Transmission Electron Microscopy. Surface functionalization of triphenylphosphonium coated zinc sulfide nanoparticles was performed by heparin and glucose coating, and they were used for intracellular analysis. Intracellular analysis is started to be performed and in progress.

Subject Keywords

Zinc sulfide nanoparticles, Triphenylphosphonium, Fluorescence spectroscopy, Mitochondria imaging

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https://hdl.handle.net/11511/91340

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

C. Mertoğlu, “The synthesis and characterization of thioglycolic acid and thiourea capped fluorescent zinc sulfide nanoparticles,” M.S. - Master of Science, Middle East Technical University, 2021.