A capillary driven microfluidic chip for SERS based hCG detection

Date

2022-01-01

Author

Ahi, Elcin Ezgi
TORUL, HİLAL
Zengin, Adem
Sucularli, Ferah
Yıldırım, Ender
Selbes, Yesim
SULUDERE, ZEKİYE
TAMER, UĞUR

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In this study, a capillary driven microfluidic chip-based immunoassay was developed for the determination of Human Chorionic Gonadotropin (hCG) protein, which is prohibited by the World Anti-Doping Agency (WADA). Here, we used antibody modified magnetic metal organic framework nanoparticles (MMOFs) as a capture prob in urine sample. MMOF captured hCG was transferred in a capillary driven microfluidic chip consisting of four chambers, and the interaction of MMOF with gold nanorods labelled with 5,5'-Dithiobis-(2-nitrobenzoic acid) (DTNB) as a Raman label was carried out in the capillary driven microfluidic chip. The movement of MMOF through first chamber to the last chamber was achieved with a simple magnet. In the last chamber of capillary driven microfluidic chip, SERS signals of DTNB molecules from the sandwich complex were recorded using a Raman spectrophotometer. The selectivity of the developed method was demonstrated by applying the same procedure for the detection of Human Luteinizing Hormone (hLH), Human Chorionic Gonadotropin Hormone (hGH) and Immunoglobulin G (IgG) protein. The regression coefficient and limit of detection obtained from the standard addition method were found as 0,9985 and 0,61 IU/L, respectively. Furthermore, the conventional ELISA method confirmed that the results obtained by the presented method were acceptable with the similarity of 97.9% in terms of average recovery value, for the detection of hCG in urine samples. The analysis system developed for target proteins will be an alternative technique such as Western Blot used in routine analysis that is expensive and time consuming.

Subject Keywords

Human chorionic gonadotropin protein, Metal organic framework, Capillary driven microfluidic chip, Surface enhanced Raman spectroscopy, HUMAN CHORIONIC-GONADOTROPIN, METAL-ORGANIC FRAMEWORK, GOLD NANOPARTICLES, MASS-SPECTROMETRY, IMMUNOASSAY, ISOFORMS, MOF

URI

https://hdl.handle.net/11511/95142

Journal

BIOSENSORS & BIOELECTRONICS

DOI

https://doi.org/10.1016/j.bios.2021.113660

Collections

Department of Mechanical Engineering, Article

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Citation Formats

E. E. Ahi et al., “A capillary driven microfluidic chip for SERS based hCG detection,” BIOSENSORS & BIOELECTRONICS, vol. 195, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95142.