Chitosan Polysaccharide Suppress Toll Like Receptor Dependent Immune Response

Date

2015-04-01

Author

Tincer, Gizem
Bayyurt Kocabaş, Banu
Arica, Yakup M.
Gursel, Ihsan

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Chitosan is a widely used vaccine or anti-cancer delivery vehicle. In this study, we investigated the immunomodulatory effect of chitosan/pIC nanocomplexes on mouse immune cells. Materials and methods: Proliferative and cytotoxic features of chitosan were tested via CCK-8 assay on RAW 264.7. IL-1b p roduction was a ssessed v ia E LISA f rom PEC s upernatants. T NF-a, and NO induction from chitosan treated RAW cells detected by ELISA and Griess assay, respectively. mRNA message levels of TLRs and cytokines on macrophages in response to chitosan/pIC nanocomplex treatments were evaluated by RT-PCR. Results: Results revealed that chitosan is non-toxic to cells, however, proliferative capacities of macrophages were reduced by chitosan administration. Mouse PECs treated with chitosan, led to NLRP3 dependent inflammasome activation as evidenced by dose-dependent IL-1b secretion. Chitosan/pIC nanocomplexes did not improve immunostimulatory action of pIC on RAW cells, since TNF-a and NO productions remained unaltered. Expression levels of several TLRs, CXCL-16 and IFN-a messages from mouse splenocytes were down regulated in response to chitosan/pIC nanocomplex treatment. Conclusion: Our results revealed that chitosan is an anti-proliferative and inflammasome triggering macromolecule on immune cells. Utilization of chitosan as a carrier system is of concern for immunotherapeutic applications.

Subject Keywords

Biomaterial, chitosan, double-stranded ribonucleic acid, innate immunity, polysaccharide, Toll like receptor 3, DELIVERY, ACTIVATION, CHITIN, INFLAMMASOME, SYSTEMS

URI

https://hdl.handle.net/11511/96121

Journal

TURKISH JOURNAL OF IMMUNOLOGY

Collections

Department of Biology, Article

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Citation Formats

G. Tincer, B. Bayyurt Kocabaş, Y. M. Arica, and I. Gursel, “Chitosan Polysaccharide Suppress Toll Like Receptor Dependent Immune Response,” TURKISH JOURNAL OF IMMUNOLOGY, vol. 3, no. 1, pp. 15–20, 2015, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/96121.