In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations

Kalem Yapar, Nisan Ece
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality. Regardless of its etiology, HCC originates from the constant wound-healing response subsequent to cell death and resulting in a cascade of inflammation. Hence, understanding the importance of inflammatory markers in HCC lies a great importance. A well-known pro-inflammatory cytokine; interleukin-6 has been numerously reported in the sera of HCC patients. This study investigated the effects of IL-6 stimulation in HCC cell line Huh7. After the presence of IL-6R was confirmed, cells were stimulated with recombinant IL-6 to check IL-6’s effect on proliferation, cellular migration, change in the stemness related transcription markers and LCSC markers and lastly, sphere formation capacity. We observed that IL-6 stimulation does not have a significant effect on proliferation, but it increases the migration rate of Huh7 cells significantly. Expression of stemness-related transcription factors such as OCT4 and NANOG, as well as LCSC markers (CD133, and EpCAM) increases significantly. Overall, we concluded that IL-6 plays an important role in HCC and the molecular mechanisms behind this effect needs to be further investigated.


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Citation Formats
N. E. Kalem Yapar, “In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations,” M.S. - Master of Science, Middle East Technical University, 2022.