In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations

Kalem Yapar, Nisan Ece
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality. Regardless of its etiology, HCC originates from the constant wound-healing response subsequent to cell death and resulting in a cascade of inflammation. Hence, understanding the importance of inflammatory markers in HCC lies a great importance. A well-known pro-inflammatory cytokine; interleukin-6 has been numerously reported in the sera of HCC patients. This study investigated the effects of IL-6 stimulation in HCC cell line Huh7. After the presence of IL-6R was confirmed, cells were stimulated with recombinant IL-6 to check IL-6’s effect on proliferation, cellular migration, change in the stemness related transcription markers and LCSC markers and lastly, sphere formation capacity. We observed that IL-6 stimulation does not have a significant effect on proliferation, but it increases the migration rate of Huh7 cells significantly. Expression of stemness-related transcription factors such as OCT4 and NANOG, as well as LCSC markers (CD133, and EpCAM) increases significantly. Overall, we concluded that IL-6 plays an important role in HCC and the molecular mechanisms behind this effect needs to be further investigated.


Molecular Biology of Liver Cancer
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Hepatocellular carcinoma (HCC) is one of the leading causes of cancer‐related mortality worldwide. Recent advances in the molecular profiling of HCC emphasize its intratumoral heterogeneity and reveal how cellular pathways are altered in favor of tumor progression. Malignant transformation of primary liver cancer is achieved through the acquisition of cancer hallmark capabilities that promote the uncontrolled proliferation of hepatocytes. In this review, the characteristics and acquired capabilities of huma...
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Hepatocellular carcinoma (HCC) is life-threatening cancer that accounts for the second leading cause of cancer-related deaths worldwide. Due to the complexity and heterogeneity of HCC, which leads to resistance to current targeted therapies, combination therapy becomes an important strategy. Since 90% of HCC arises from chronic liver inflammation, HCC drugs combined with non-steroid anti- inflammatory drugs (NSAIDs) are considered an important strategy to increase treatment efficacy. In this study, we aimed...
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Cancer is a major health problem in developing world with mostly unsufficient treatment. Cancer prevention through dietary modification appears to be a practical and cost effective possibility. The aim of present study is to investigate the chemical components of “Urtica diocia,L (U. diocia) grown in Turkey” and the possible protective potential of its aqueous extract against breast cancer cell lines. U. diocia was extracted by maceration method which was performed for 6,12, 24, and 36 hours, at 50°C, 37°C,...
Synthesis of poly (dl-lactic-co-glycolic acid) coated magnetic nanoparticles for anti-cancer drug delivery
Tansık, Gülistan; Gündüz, Ufuk; Department of Biology (2012)
One of the main problems of current cancer chemotherapy is the lack of selectivity of anti-cancer drugs to tumor cells which leads to systemic toxicity and adverse side effects. In order to overcome these limitations, researches on controlled drug delivery systems have gained much attention. Nanoscale based drug delivery systems provide tumor targeting. Among many types of nanocarriers, superparamagnetic nanoparticles with their biocompatible polymer coatings can be targeted to an intented site by an extern...
Identification of biomarkers associated with partial epithelial to mesenchymal transition in the secretome of slug over-expressing hepatocellular carcinoma cells
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Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. Complete epithelial to mesenchymal transition (EMT) has long been considered as a crucial step for metastasis initiation. It has, however, become apparent that many carcinoma cells can metastasize without complete loss of epithelial traits or with incomplete gain of mesenchymal traits, i.e., partial EMT. Here, we aimed to determine the similarities and differences between complete and partial EMT through over-expr...
Citation Formats
N. E. Kalem Yapar, “In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations,” M.S. - Master of Science, Middle East Technical University, 2022.