In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations

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2022-1-26

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Kalem Yapar, Nisan Ece

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Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality. Regardless of its etiology, HCC originates from the constant wound-healing response subsequent to cell death and resulting in a cascade of inflammation. Hence, understanding the importance of inflammatory markers in HCC lies a great importance. A well-known pro-inflammatory cytokine; interleukin-6 has been numerously reported in the sera of HCC patients. This study investigated the effects of IL-6 stimulation in HCC cell line Huh7. After the presence of IL-6R was confirmed, cells were stimulated with recombinant IL-6 to check IL-6’s effect on proliferation, cellular migration, change in the stemness related transcription markers and LCSC markers and lastly, sphere formation capacity. We observed that IL-6 stimulation does not have a significant effect on proliferation, but it increases the migration rate of Huh7 cells significantly. Expression of stemness-related transcription factors such as OCT4 and NANOG, as well as LCSC markers (CD133, and EpCAM) increases significantly. Overall, we concluded that IL-6 plays an important role in HCC and the molecular mechanisms behind this effect needs to be further investigated.

Subject Keywords

HCC, IL-6, cancer stem cells

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https://hdl.handle.net/11511/96691

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

N. E. Kalem Yapar, “In vitro autocrine secretion of inflammatory cytokines in response to drug stress in HCC and LCSC populations,” M.S. - Master of Science, Middle East Technical University, 2022.