Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure

Isik, Melis
Eylem, Cemil Can
Camci, Can Berk
Bilgin, Sila
Akkulak, Merve
Adalı, Orhan
Okesola, Babatunde O.
Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.


Characterization and identification of human mesenchymal stem cells at molecular level
Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012)
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Mineral-Doped Poly(L-lactide) Acid Scaffolds Enriched with Exosomes Improve Osteogenic Commitment of Human Adipose-Derived Mesenchymal Stem Cells
Gandolfi, Maria Giovanna; Gardin, Chiara; Zamparini, Fausto; Ferroni, Letizia; Esposti, Micaela Degli; Parchi, Greta; Ercan, Batur; Manzoli, Lucia; Fava, Fabio; Fabbri, Paola; Prati, Carlo; Zavan, Barbara (2020-03-01)
Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced phase separation technique, as materials for bone regeneration. The aim of this study was to investigate the effect of mineral-doped PLA-based porous scaffolds enriched with ex...
Role of Vibrational Spectroscopy in Stem Cell Research
Aksoy, Ceren; Severcan, Feride (2012-01-01)
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
Enhanced expression of HNF4 alpha during intestinal epithelial differentiation is involved in the activation of ER stress
Tuncer, Sinem; Sade-Memişoğlu, Aslı; Keşküş, Ayşe Gökçe; Sheraj, Ilir; Güner, Güneş; Akyol, Aytekin; Banerjee, Sreeparna (WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, 2019-12)
Intestinal epithelial cells are derived from stem cells at the crypts that undergo differentiation into transit-amplifying cells, which in turn form terminally differentiated enterocytes as these cells reach the villus. Extensive alterations in both transcriptional and translational programs occur during differentiation, which can induce the activation of cellular stress responses such as ER stress-related unfolded protein response (UPR) and autophagy, particularly in the cells that are already committed to...
Effect of double growth factor release on cartilage tissue engineering
Ertan, Ayse Burcu; Yilgor, Pinar; Bayyurt, Banu; Calikoglu, Ayse Ceren; Kaspar, Cigdem; Kök, Fatma Neşe; KÖSE, GAMZE; Hasırcı, Vasıf Nejat (2013-02-01)
The effects of double release of insulin-like growth factor I (IGF-I) and growth factor 1 (TGF1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF1, respectively. On tissue culture polystyrene (TCPS), TGF-1 released from PNIPAM nanoparticles was found to ...
Citation Formats
B. DERKUŞ et al., “Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure,” ADVANCED BIOLOGY, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/96905.