Coaxial electrospinning of composite mats comprised of core/shell poly(methyl methacrylate)/silk fibroin fibers for tissue engineering applications

Atila, Deniz
HASIRCI, Vasıf Nejat
Tezcaner, Ayşen
© 2022 Elsevier LtdMimicking extracellular matrix (ECM) of native tissue by tissue-engineered constructs is critical to induce regeneration of the damaged site. In this study, coaxial electrospinning of core/shell poly(methyl methacrylate) (PMMA)/silk fibroin (SF) fibers was optimized for the first time to provide ECM-like microenvironment for new tissue formation by utilization of a new collector design for obtaining homogeneously deposited mats from the collector screen. SF-shell was produced to increase cell-affinity of fiber surfaces whereas PMMA-core was designed to support the tissue mechanically during regeneration. PMMA/SF membranes were characterized. Morphology of core/shell PMMA/SF fibers resembled neat SF (ribbon-like) fibers rather than neat PMMA (cylindrical) fibers since SF constituted the shell part. The average diameter of PMMA/SF fibers (2.51 μm) lied in between the neat counterparts (PMMA: 2.40 μm and SF: 2.84 μm). The morphological and chemical properties affected the water contact angle and porosity of the mats, leading to the highest hydrophilicity for SF mats and the highest porosity for PMMA mats among the groups. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) confirmed the core/shell structure of PMMA/SF fibers. The combination of these remarkably different polymers (synthetic, hydrophobic, brittle PMMA and natural, hydrophilic, flexible SF) resulted in intermediate mechanical properties of PMMA/SF mats both in dry and wet conditions by preserving fibrous and porous structures in the core/shell form unlike the neat mats. Thermogravimetric analyses (TGA) showed the highest mass loss for PMMA/SF mats which lost 13.9% of their initial weight unlike the neat counterparts. In vitro hydrolytic & enzymatic degradation studies revealed that PMMA/SF had the weight loss between those observed for SF and PMMA mats in the presence and absence of enzymes while possessing the highest water uptake capacity. SEM examinations of mats after 14 days of hydrolytic degradation demonstrated the SF-shell of the fibers were fused at the intercept points of the PMMA/SF network while the PMMA-core acted as a separating backbone and preserved fibrous, and hence porous architecture of the mats. Cell culture studies demonstrated that human dental pulp stem cells (DPSC) were able to attach and proliferated on PMMA/SF mats while a lower degree of cell spreading on PMMA mats was observed. DPSC adhesion was improved by SF-shell in PMMA/SF group. In conclusion, electrospun composite mats composed of core/shell PMMA/SF fibers could be considered a promising candidate for tissue engineering applications and drug delivery strategies.
Journal of the Mechanical Behavior of Biomedical Materials


Free standing layer-by-layer films of polyethyleneimine and poly(l-lysine) for potential use in corneal stroma engineering
Altay, Gizem; Hasırcı, Vasıf Nejat; Khademhosseini, Ali; Department of Biomedical Engineering (2011)
In this study we fabricated free standing multilayer films of polyelectrolyte complexes for potential use in tissue engineering of corneal stroma by using the layer-by-layer (LbL) approach. In the formation of these LbL films negatively charged, photocrosslinkable (methacrylated) hyaluronic acid (MA-HA) was used along with polycations polyethyleneimine (PEI) and poly(L-lysine) (PLL). Type I collagen (Col) was blended in with PLL for improving the water absorption and cell attachment properties of the films....
Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes
Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06)
This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...
Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes
Garsha, Bahrieh; Erdem, Murat; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2013-10-31)
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce th...
3D porous PCL-PEG-PCL / strontium, magnesium and boron multi-doped hydroxyapatite composite scaffolds for bone tissue engineering
Yedekçi, Buşra; Tezcaner, Ayşen; Evis, Zafer (2022-01-01)
Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped HA that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HA and PCL-PEG-PCL copolyme...
Polyelectrolyte multilayer films as substrates for photoreceptor cells
Tezcaner, Ayşen; Boulmedais, F; SAHEL, JOSE; SCHAAF, PIERRE; VOEGEL, JEAN CLAUDE; LAVALLE, PHILIPPE (2006-01-01)
Reconstruction of extracellular matrix substrates for delivery of functional photoreceptors is crucial in pathologies such as retinal degeneration and age-related macular degeneration. In this study, we assembled polyelectrolyte films using the layer-by-layer deposition method. The buildup of three different films composed of poly(L-lysine)/ chondroitin sulfate (PLL/CSA), poly(L-lysine)/poly(styrenesulfonate) (PLL/PSS), or poly(L-lysine)/hyaluronic acid (PLL/HA) was followed by means of quartz crystal micro...
Citation Formats
D. Atila, V. N. HASIRCI, and A. Tezcaner, “Coaxial electrospinning of composite mats comprised of core/shell poly(methyl methacrylate)/silk fibroin fibers for tissue engineering applications,” Journal of the Mechanical Behavior of Biomedical Materials, vol. 128, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: