Food spoilage monitoring using carbon nanodots and uv irradiation reinforced colorimetric fish gelatin films

Download

Münevver Beyza Kılıç MS thesis.pdf

Date

2022-9

Author

Kılıç, Münevver Beyza

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

255
views

312
downloads

Foodborne diseases can lead to serious health and economic losses. Therefore, research priority has been given to the efforts to ensure food safety in recent years. Within this framework, effective and user-friendly smart packaging technologies need to be developed and integrated into food packaging applications. The proposed project aims to develop a biocompatible colorimetric film that can be integrated into food packaging where the consumer can easily obtain a real-time freshness assessment of food. Fish gelatin (FG) will be used as support material in colorimetric film fabrication. Despite the poor mechanical properties of FG, it can be produced more economically compared to the other types of gelatins. One of the main objectives of the proposed project is to strengthen the crosslinking structure of FG using carbonyl functionalized carbon nanodots (C-dots). The C-dots obtained through the pyrolysis of citric acid will be used for the chemical crosslinker, and ultraviolet irradiation will be used to improve the crosslinking structure of FG as a physical crosslinker. The effect of these crosslinkers on the barrier, mechanical, structural, and optical properties of FG films was investigated. The integration of C-dots with UV irradiation improved these properties. In the structure of the colorimetric film, natural color pigment anthocyanins, which can be obtained by extraction from red cabbage, will be used as the sensing biomolecule. The working principle of a colorimetric film is based on a color change that anthocyanins pigments exhibit to change in the concentration of the total volatile basic nitrogen gases that release during food spoilage. The kinetic colorimetric responses of FG films against ammonia gases were investigated to simulate the spoilage of chicken breasts and determine the ammonia sensitivity of FG films. UV-treated FG films containing 100 mg/l C-dots (FG-UV-100) indicated the best physicochemical and colorimetric properties. Consequently, the color change of FG-UV-100 films was correlated with microbial growth and total volatile basic nitrogen (TVB-N) release during the 9-day storage of chicken breast samples at 4oC. The main objective of this study was to identify the appropriate methods and to integrate fish gelatin and C-dots into smart packaging systems to expand their usage areas and turn them into higher value-added products.

Subject Keywords

Fish gelatin, Carbon dots, UV irradiation, Anthocyanins, Colorimetric Films

URI

https://hdl.handle.net/11511/99528

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Development of anthocyanins-based colorimetric film under visible light irradiation for the detection of food spoilage Kit, İdil; Kahyaoğlu, Leyla Nesrin; Şahin, Serpil; Department of Food Engineering (2023-1-27) Food spoilage causes serious problems including foodborne diseases, food waste, and, economic burdens. For this reason, research on ensuring food safety has been increasing in recent years. The most commonly followed approach is the integration of intelligent packaging technologies. Although colorimetric films are usually fabricated with heat treatment, some pigments and biomolecules can be sensitive to heat. Therefore, this study aimed to produce visible irradiated colorimetric films using grape anthocyani...
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection Bulut, Onur; Öktem, Hüseyin Avni; Department of Biology (2014) Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current...
Generation of aptamer against salmonella serovar enteritidis and development of aptamer-based capillary biosensor Bayraç, Ceren; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014) Food poisoning became one of the most important diseases that threaten to human health. Among all the food-borne pathogens, Salmonella is the most common cause of food-borne infectious disease in the world. Among over 2500 serovars, Salmonella enterica serovar Enteritidis is a common foodborne pathogen associated with human diseases. Limited and time consuming diagnostic techniques such as culturing method or polymerase chain reaction lead to increase in the demands of new detection methods. Therefore, in t...
Anatolan sage Salva frutcosa nhbts cytosolc glutathone-s-transferase actvty and colon cancer cell prolferaton ALTAY, AHMET; KILIÇ SÜLOĞLU, AYSUN; Celep, Gulcin Sagdicoglu; SELMANOĞLU, GÜLDENİZ; Bozoglu, Faruk (Springer Science and Business Media LLC, 2019-06-01) Colorectal cancer is one of the leading causes of death worldwide, and healthy diet is critical in preventing it. This study intends to investigate phenolic profile, antioxidant, and anti-proliferative activity as well as Glutathione S-transferase (GST) inhibition activity of water extract of Salvia fruticosa Mill. (SF.) HPLC analysis revealed that the main bioactive ingredients of SF were rosmarinic and caffeic acid. Total phenolic and flavonoid contents were 425.1mg GAE/g extract and 94.7mg CE/g extract, ...
How can we improve foodborne disease surveillance systems: A comparison through EU and US systems Yenı, F.; Acar, Sibel; Soyer, Yeşim; Alpas, Hami (2017-01-01) Foodborne diseases remain a major threat to global public health. Problems in detecting the threats at early stages in the supply chain due to defects in surveillance systems cannot be ignored. The primary goal of this article is to evaluate the capabilities of foodborne disease surveillance systems known for their effectiveness and functionality in order to highlight solutions for preventing and managing the outbreaks more effectively. For this purpose, early detection, notification and monitoring systems ...

Citation Formats

M. B. Kılıç, “Food spoilage monitoring using carbon nanodots and uv irradiation reinforced colorimetric fish gelatin films,” M.S. - Master of Science, Middle East Technical University, 2022.