Muhammed, Yasser
Motameni, Ali
Gürbüz, Rıza
All rights reserved.The purpose of this study is to synthesize and characterize the mesoporous nanostructure of Hydroxyapatite (HA), evaluate the impact of the addition of sulphate ions (SO42-) on the microstructure properties of HA, and assess the ability of the prepared materials to be used as a carrier for Bovine serum albumin (BSA) protein. This study successfully synthesized 0.07 mole of SO42- substituted HA using the reflux microwave-assisted wet precipitation method and fully characterized it using modern techniques. In particular, XRD, XPS, Raman, FESEM, and BET techniques were used to explore the micro-structure properties of materials. The results shows that the addition of SO42- ions amplified the lattice parameters and surface area of HA, and the particles shape was changed from semi-spherical to rod like shape. Adsorption and desorption of protein molecules were evaluated using BSA protein for 24 h at 37°C. BSA loading capacity and release behaviours were significantly improved with the incorporation of SO42- ions. The SO4- HA materials hold a promising future for being used as a carrier for protein molecules.
Ceramics - Silikaty


Biomimetic preparation of HA precursors at 37 degrees C in urea- and enzyme urease-containing synthetic body fluids
Bayraktar, D; Tas, AC (1999-12-01)
An important inorganic phase of synthetic bone applications, calcium hydroxyapatite (HA, Ca-10(PO4)(6)(OH)(2)), was prepared as a single-phase and sub-micron bioceramic powder. Carbonated HA precursors were synthesized from calcium nitrate tetrahydrate and diammonium hydrogen phosphate salts dissolved in "synthetic body fluid" (SBF) solutions, containing urea (H2NCONH2) and enzyme urease, under the biomimetic conditions of 37 degrees C and pH 7.4, by using a novel chemical precipitation technique.
Polyethylene oxide-hydroxyapatite composite and microporous polyethylene oxide-polymethacrylic acid polymer complex preparation and characterization
Banat, Raid M I; Tinçer, Teoman; Department of Chemistry (2002)
The first part of this study covers the crosslinking of polyethylene oxide (PEO) and its composite with calcium hydroxyapatite (HA) and their mechanical, swelling and morphological properties. Sheets of neat PEO (two different molecular weights) and composites of PEO-HA were prepared by compression molding. The composites and PEO thick sheets were crosslinked with exposure of UV- irradiation in presence of acetophenone (AP) for different periods of time. This simple method of crosslinking, UV-irradiation in...
Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads
A carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertas...
Mesoporous strontium doped nano sized sulphate hydroxyapatite as a novel biomaterial for bone tissue applications
Alshemary, Ammar Z.; Pazarçeviren, Ahmet Engin; Tezcaner, Ayşen; Evis, Zafer (Royal Society of Chemistry (RSC), 2016)
In this study a novel nano-structured hydroxyapatite (HA) incorporated with different fractions of Sr2+ and SO42− ions has been synthesized using the wet precipitation method and characterized.
Microorganism mediated stereoselective bio-oxidation and bio-hydrogenation reactions and thiamine pyrophosphate dependent enzyme catalyzed enantioselective acyloin reactions
Sopacı, Şaziye Betül; Demir, Ayhan Sıtkı; Department of Biotechnology (2009)
In this study various microbial and enzymatic methods developed for enantioselective acyloin synthesis for preparation of some pharmaceutically important intermediates. By performing Aspergillus flavus (MAM 200120) mediated biotransformation, enantioselective bio-oxidation of meso-hydrobenzoin was achieved with a high ee value (76%). Racemic form of hydrobenzoin was also employed for the same bio-oxidation process and this bioconversion was resulted in accumulation of meso form (>90% yield) confirming the s...
Citation Formats
A. Z. ALSHEMARY, Y. Muhammed, N. A. SALMAN, A. Motameni, and R. Gürbüz, “MESOPOROUS SULPHATE DOPED HYDROXYAPATITE NANOPARTICLES FOR CONTROLLED RELEASE OF PROTEINS,” Ceramics - Silikaty, vol. 66, no. 4, pp. 447–452, 2022, Accessed: 00, 2023. [Online]. Available: