Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Integrated biomimetic scaffolds for soft tissue engineering
Download
index.pdf
Date
2006
Author
Güven, Sinan
Metadata
Show full item record
Item Usage Stats
181
views
63
downloads
Cite This
Tissue engineering has the potential to create new tissue and organs from cultured cells for transplantation. Biodegradable and biocompatible scaffolds play a vital role in the transfer of the cultured cells to a new tissue. Various scaffolds for soft tissue engineering have been developed, however there is not any structure totally mimicking the natural extracellular matrix (ECM), ready to use. In this study biodegradable and biocompatible scaffolds were developed from natural polymers by tissue engineering approach and tested in vitro. Scaffolds (SCAF) were prepared with freeze drying and composed of chitosan, gelatin and dermatan sulfate. Polymer solutions were treated with different stirring rates (500 rpm and 2000 rpm), freezing temperatures (-20 °C and -80 °C) and molding (cylindrical mold and petri dish) to achieve porous structure in order to provide sufficient space for cell growth and extracellular matrix production. Among the prepared scaffolds at different conditions, the scaffolds prepared at 500 rpm and frozen at -80 °C, (SCAF-1), was chosen for further studies. These scaffolds achieved 0.512 MPa tensile strength, with 9.165 MPa tension modulus and 3.428 MPa compression modulus. Besides in lysozyme containing degradation medium they conserved their integrity and lost about 30 % of their initial weight in 30 days period. Mechanical and enzymatic degradation tests showed that scaffolds have physical integrity for the tissue engineering applications. To mimic the natural tissue and enhance cell growth, biologically active arginine glycine - aspartic acid - serine (RGDS) peptides and platelet derived growth factor-BB (PDGF-BB) were immobilized on the SCAF-1. Fibroblast cells were seeded on the scaffolds containing RGDS, (SCAF-1-RGDS), and PDGF-BB, (SCAF-1-RGDS-PDGF), and incubated in media either free of serum or containing serum. Scaffolds immobilized with RGDS and PDGF-BB had the highest attached cell number by the day 15. Florescence microscopy studies also indicated that RGDS and RGDS-PDGF modified scaffolds were more suitable than controls, (SCAF-1), for cell growth and proliferation. According to scanning electron microscopy (SEM) results, modified scaffolds demonstrated better cell morphology and attachment of cells. Based on the obtained results, it can be concluded that RGDS-PDGF immobilized chitosan-gelatin-dermatan sulfate systems have a great potential to be used as a scaffold for soft tissue engineering applications.
Subject Keywords
Bioengineering.
URI
http://etd.lib.metu.edu.tr/upload/3/12607436/index.pdf
https://hdl.handle.net/11511/16271
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Native extracellular matrix/fibroin hydrogels for adipose tissue engineering with enhanced vascularization
Kayabolen, Alisan; Keskin, Dilek; Aykan, Andac; Karslioglu, Yildirim; Zor, Fatih; Tezcaner, Ayşen (IOP Publishing, 2017-06-23)
Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, vascularization is needed since nutrients and oxygen cannot reach cells in thick implants by diffusion. Obtaining a biocompatible scaffold with good mechanical properties is another problem. In this study, we aimed to develop thick and vascularized adipose tissue constructs supporting cell viability and adipose tissue regeneration. Hydrogels were prepared by mixing rat decellularized adipose tissue (DAT) and si...
Advanced cell therapies with and without scaffolds
Demirbag, Birsen; HURİ, PINAR; KÖSE, GAMZE; Buyuksungur, Arda; Hasırcı, Vasıf Nejat (2011-12-01)
Tissue engineering and regenerative medicine aim to produce tissue substitutes to restore lost functions of tissues and organs. This includes cell therapies, induction of tissue/organ regeneration by biologically active molecules, or transplantation of in vitro grown tissues. This review article discusses advanced cell therapies that make use of scaffolds and scaffold-free approaches. The first part of this article covers the basic characteristics of scaffolds, including characteristics of scaffold material...
Finite element investigation of mechanical interaction of dental implants with bone
Eser, Atılım; Tönük, Ergin; Department of Mechanical Engineering (2007)
During the last years, biomechaniccs, the understanding of the very complex mechanical behavior of living tissue, becomes a very important field of research of the wide bravch of mechanichs. Thia study is very important, for instance , to improve the design of implants, with the important social and economical impact. one of important challenges in implant design is to model the complex material behavior of the bone. Remodeling of the bone due to the lodaing is one of the essential behaviors of the bone whi...
Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering
Rad, Reza Moonesi; ALSHEMARY, AMMAR ZEIDAN GHAILAN; Evis, Zafer; Keskin, Dilek; Tezcaner, Ayşen (IOP Publishing, 2020-11-01)
In this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B(2)O(3)incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, por...
3D Printed Hydrogel Multiassay Platforms for Robust Generation of Engineered Contractile Tissues
Christensen, Rie Kjaer; Laier, Christoffer von Halling; Kızıltay, Aysel; Wilson, Sandra; Larsen, Niels Bent (American Chemical Society (ACS), 2020-02-01)
We present a method for reproducible manufacture of multiassay platforms with tunable mechanical properties for muscle tissue strip analysis. The platforms result from stereolithographic 3D printing of low protein-binding poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Contractile microtissues have previously been engineered by immobilizing suspended cells in a confined hydrogel matrix with embedded anchoring cantilevers to facilitate muscle tissue strip formation. The 3D shape and mechanical properties...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. Güven, “Integrated biomimetic scaffolds for soft tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2006.