Electrospun nanofibrous scaffolds for tissue engineering

Ndreu, Albana
In this study a microbial polyester, poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV), and its blends were wet or electrospun into fibrous scaffolds for tissue engineering. Wet spun fiber diameters were in the low micrometer range (10-50 μm). The polymer concentration and the stirring rate affected the properties the most. The optimum concentration was determined as 15% (w/v). Electrospun fiber diameters, however, were thinner. Solution viscosity, potential, distance between the syringe tip and the collector, and polymer type affected the morphology and the thickness of beads formed on the fibers. Concentration was highly influential; as it increased from 5% to 15% (w/v) fiber diameter increased from 284 ± 133 nm to 2200 ± 716 nm. Increase in potential (from 20 to 50 kV) did not lead to the expected decrease in fiber diameter. The blends of PHBV8 with lactide-based v polymers (PLLA, P(L,DL-LA) and PLGA (50:50)) led to fibers with less beads and more uniform thickness. In vitro studies using human osteosarcoma cells (SaOs-2) revealed that wet spun fibers were unsuitable because the cells did not spread on them while all the electrospun scaffolds promoted cell growth and penetration. The surface porosities for PHBV10, PHBV15, PHBV-PLLA, PHBV-PLGA (50:50) and PHBV-P(L,DL)LA were 38.0±3.8, 40.1±8.5, 53.8±4.2, 50.0±4.2 and 30.8±2.7%, respectively. Surface modification with oxygen plasma treatment slightly improved the cell proliferation rates. Consequently, all scaffolds prepared by electrospinning revealed a significant potential for use in bone tissue engineering applications; PHBV-PLLA blend appeared to yield the best results.


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An N-halamine precursor, 3-(5'-methyl-5'-hydantoinyl)acetanilide (1), was synthesized in our laboratory and loaded onto electrospun polyacrylonitrile fiber to prepare nanosized biocidal materials, which could be rendered antimicrobial by exposure to household bleach. Differential scanning calorimetry was used to study the thermal properties of the nanofibers with and without the N-halamine precursor and its chlorinated derivative loaded. Scanning electron microscopy demonstrated that the ultrafine fibers fo...
Doku mühendisliği yöntemi ile kollajen ve nacmc kullanılarak çift katmanlı deri modeli oluşturulması
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TÜBİTAK tarafından desteklenen bu çalışmada, insan derisini taklit etmek amacıyla, gözenekli kollajen ve sodyum karboskimetilselüloz (NaCMC) iskeleler kullanılarak iki katmanlı deri modelleri başarıyla üretilmiştir. Geliştirilen deri modelinde, gözenekli kollajen iskelenin dış katman olan epidermal tabakasını, ve gözenek boyutu dış katmana göre daha büyük olan NaCMC iskelenin ise iç katman olan dermal tabakayı oluşturması planlanmıştır. Hazırlanan iskeleler, sulu ortamda dayanıklı olabil...
Electrospinning of chitosan/poly(lactic acid-co-glycolic acid)/hydroxyapatite composite nanofibrous mats for tissue engineering applications
Endoğan Tanır, Tuğba; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2014-11-01)
Electrospinning, which is a fiber fabrication technique using electrical forces to produce fibers with diameters ranging from nanometers to several micrometers, can be used to prepare materials mimicking the extracellular matrix proteins for potential use as tissue engineering scaffolds. In this study, nanofibrous mats of chitosan (CH) and poly(lactic acid-co-glycolic acid) (PLGA) having fiber diameters between 167 to 525 nm, and containing hydroxyapatite (HAp), were prepared by electrospinning technique. M...
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Karahaliloglu, Zeynep; Ercan, Batur; DENKBAŞ, EMİR BAKİ; Webster, Thomas J. (2015-01-01)
As an effort to create the next generation of improved skin graft materials, in this study, we modified the surfaces of a previously investigated material, silk fibroin, using a NaOH alkaline treatment to obtain a biologically inspired nanofeatured surface morphology. Such surfaces were characterized for roughness, energy, and chemistry. In addition, keratinocyte (skin-forming cells) adhesion and proliferation on such nanofeatured silk fibroin wound dressings were studied in an initial attempt to determine ...
Electrical transport, optical and thermal properties of polyaniline-pumice composites
YILMAZ, KORAY; Akgoz, A.; Cabuk, M.; Karaagac, H.; KARABULUT, ORHAN; YAVUZ, Mustafa (2011-11-01)
In this study, electrical conductivity, photoconductivity, absorbance and thermal properties of polyaniline (PANI) and polyaniline-pumice composites were investigated. Temperature dependent conductivity and photoconductivity measurements were carried out in the temperature range of 80-400K. The measurements revealed that the dominant conduction mechanisms in polyaniline and 15% pumice doped composite were hopping conduction. The low activation energies calculated for 36% pumice doped composite indicated tha...
Citation Formats
A. Ndreu, “Electrospun nanofibrous scaffolds for tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2007.