Comparative effects of emodin on biological activities of MCF-7 and MDA-231 cell lines

Download

index.pdf

Date

2010

Author

Sakallı, Elif

Metadata

Show full item record

Item Usage Stats

203
views

80
downloads

Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a phytoestrogenic component of Rheum plant extracts which has been used for medical treatment since ancient times. It has been shown to have anti-inflammatory and anti-cancer effects. In our research, we aimed to study the biological effects of emodin on MCF-7 and MDA-231 cell lines. Cytotoxicity assays showed that emodin treatment for 48hours caused a concentration dependent decrease in viable cell numbers of both cell lines. As determined by cell counting with tryphan blue, IC50 values were 8.40 and 12.17 µg/ml for MCF-7 and MDA-231 cells, respectively. Apoptotic effects of emodin was investigated by measuring the changes in apoptotic and antiapoptotic gene expressions by qRT-PCR. In MCF-7 cells, Bax expression increased with increasing emodin concentrations, while Bcl-2 expression was downregulated. Bax/Bcl-2 ratio was calculated as 9.2 fold at 10µg emodin/ml treatment for 48 hours, indicating stimulation of apoptosis. However, Bax/Bcl-2 ratio was found 1.6 fold for MDA-231 cells. These results were in accordance with the results obtained from microarray analysis of related gene expressions. MCF-7 cells were more apoptotic in comparison to MDA-231 cells. DNA fragmentation was observed in MCF-7 cells by TUNEL method. GST enzyme activity that was measured using CDNB as substrate, was increased 100% with respect to control up to 5µg emodin/ml treatment of MCF-7 cells for 48 hours. However, effect of emodin on GST activity in MDA-231 cells was found insignificant. According to microarray analysis results, emodin affected the gene expressions of GST isozymes in MCF-7 cells much more than in MDA-231 cells.

Subject Keywords

Emodin.

URI

http://etd.lib.metu.edu.tr/upload/12612825/index.pdf
https://hdl.handle.net/11511/20291

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Effect of emodin on doxorubicin-induced cytotoxicity and apoptosis on MCF-7 and MCF-10a cells Kaya, Bade; Güray, Tülin; Uyar, Pembegül; Department of Biology (2014) Emodin (3-Methyl-1,6,8-trihydroxyanthraquinone), is a phytoestrogenic component of Rheum and Polygonum plant extracts which has been used to treat several diseases since ancient times. It has been shown to have anti-microbial, anti-oxidant and anti-cancer effects in nature. The anti-tumor drug doxorubicin, a widely used chemotherapeutic agent, is used for the treatment of many cancer types including lung, gastric, ovarian and breast cancer. In this study, the effects of pre-, co- and alone treatment of doxo...
Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase Çoruh, Nursen; Ozgokce, F. (2007-01-01) Therapeutic effects of several medicinal plants and vegetables, which are commonly used as food and in folk medicine against many disease, are well known. Antioxidant capacities of Heracleum persicum Desf., Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. species from Apiaceae family were evaluated by determining their effects on DPPH radical scavenging, and lipid peroxidation inhibition, as well as their total phenolic contents. Potential natural glutathione-S-transferase inhibitors have gain...
Effect of hydrogenation conditions on rheological and micro-structural properties of fats Başkoçak, Altuğ; Mert, Behiç; Department of Food Engineering (2011) Hydrogenation is one of the most applied techniques in the fats and oils industry to produce wide range of hardened fats with different physical and chemical properties. Each different combination of hydrogenation conditions serves products of different rheological and micro-structural properties. Therefore, the purpose of this study is to examine the effect of different industrially available catalysts on rheological and micro-structural properties of hydrogenated fats. Three different catalysts were used ...
Anticarcinogenic effects of the ethanolic extract from salix aegyptiaca l. in colon cancer, involvement of akt pathway Enayat, Shabnam; Banerjee, Sreeparna; Department of Biology (2013) Salix aegyptiaca has been used for centuries as a medicinal plant for various health disorders. Our previous study demonstrated high contents of phenolics and antioxidants in its bark many of those are famed for their anti-inflammatory properties. Since inflammation frequently accompanies the progress of colorectal cancer, in the current study we showed that the ethanolic extract of bark (EEB) from S.aegyptiaca exerted strong anti-proliferative effects on several cancer cells but not on non-cancerous CCD-18...
Optimization of hypericin extraction from hypericum perforatum L. tissues and evaluation of its applicability in dye-sensitized solar cells / Aktuna, Özge; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014) Medicinal plants which have been widely used in folk medicine are known to contain important biologically active compounds. Hypericum perforatum is one of the medicinal plants that grows in Europe, Western Asia and Northern Africa and is distinguished by its golden yellow flowers. The common name of the plant is St. John’s wort. As a practical folk-remedy, it has been used widely to heal wounds, remedy kidney troubles, and alleviate nervous disorders. Nowadays, many application areas of hypericin are discov...

Citation Formats

E. Sakallı, “Comparative effects of emodin on biological activities of MCF-7 and MDA-231 cell lines,” M.S. - Master of Science, Middle East Technical University, 2010.