Effect of emodin on doxorubicin-induced cytotoxicity and apoptosis on MCF-7 and MCF-10a cells

Download
2014
Kaya, Bade
Emodin (3-Methyl-1,6,8-trihydroxyanthraquinone), is a phytoestrogenic component of Rheum and Polygonum plant extracts which has been used to treat several diseases since ancient times. It has been shown to have anti-microbial, anti-oxidant and anti-cancer effects in nature. The anti-tumor drug doxorubicin, a widely used chemotherapeutic agent, is used for the treatment of many cancer types including lung, gastric, ovarian and breast cancer. In this study, the effects of pre-, co- and alone treatment of doxorubicin and emodin in MCF-7 and MCF-10A cell lines were investigated. MCF-7 and MCF-10A cells were cultured in the presence of various concentrations of emodin and doxorubicin at 6, 24 and 72 hours. The effect of emodin varies according to the presence of doxorubicin (pre-treatment, co-treatment, post-treatment) on both cell lines. Emodin pre-treatment (0.4 and 4 µM) for 24-hour prior to doxorubicin treatment (0.1, 0.83, 2.5 µM) caused to increase in cell viability of MCF-10A cells, comparing to doxorubicin alone treatment. Whereas no effect was observed in MCF-7 cells. Emodin post- and co-treatment with doxorubicin for 72-hour inhibited the survival of MCF-7 and MCF-10A cells in a concentration dependent manner, shown by trypan blue and XTT. Apoptotic effects of doxorubicin and emodin were investigated by flow cytometry. While emodin (0.4 µM) did not induce apoptosis in both cell lines, doxorubicin alone, pre- and co-treatment (0.83 µM) with emodin (0.4 µM) induced late apoptosis/necrosis in MCF-7 and MCF-10A cells. Mitochondrial membrane potential loss was not observed after doxorubicin and emodin treatment in both cell lines.
Citation Formats
B. Kaya, “Effect of emodin on doxorubicin-induced cytotoxicity and apoptosis on MCF-7 and MCF-10a cells,” M.S. - Master of Science, Middle East Technical University, 2014.