Effect of emodin on doxorubicin-induced cytotoxicity and apoptosis on MCF-7 and MCF-10a cells

Kaya, Bade
Emodin (3-Methyl-1,6,8-trihydroxyanthraquinone), is a phytoestrogenic component of Rheum and Polygonum plant extracts which has been used to treat several diseases since ancient times. It has been shown to have anti-microbial, anti-oxidant and anti-cancer effects in nature. The anti-tumor drug doxorubicin, a widely used chemotherapeutic agent, is used for the treatment of many cancer types including lung, gastric, ovarian and breast cancer. In this study, the effects of pre-, co- and alone treatment of doxorubicin and emodin in MCF-7 and MCF-10A cell lines were investigated. MCF-7 and MCF-10A cells were cultured in the presence of various concentrations of emodin and doxorubicin at 6, 24 and 72 hours. The effect of emodin varies according to the presence of doxorubicin (pre-treatment, co-treatment, post-treatment) on both cell lines. Emodin pre-treatment (0.4 and 4 µM) for 24-hour prior to doxorubicin treatment (0.1, 0.83, 2.5 µM) caused to increase in cell viability of MCF-10A cells, comparing to doxorubicin alone treatment. Whereas no effect was observed in MCF-7 cells. Emodin post- and co-treatment with doxorubicin for 72-hour inhibited the survival of MCF-7 and MCF-10A cells in a concentration dependent manner, shown by trypan blue and XTT. Apoptotic effects of doxorubicin and emodin were investigated by flow cytometry. While emodin (0.4 µM) did not induce apoptosis in both cell lines, doxorubicin alone, pre- and co-treatment (0.83 µM) with emodin (0.4 µM) induced late apoptosis/necrosis in MCF-7 and MCF-10A cells. Mitochondrial membrane potential loss was not observed after doxorubicin and emodin treatment in both cell lines.


Comparative effects of emodin on biological activities of MCF-7 and MDA-231 cell lines
Sakallı, Elif; İşcan, Mesude; Department of Biochemistry (2010)
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a phytoestrogenic component of Rheum plant extracts which has been used for medical treatment since ancient times. It has been shown to have anti-inflammatory and anti-cancer effects. In our research, we aimed to study the biological effects of emodin on MCF-7 and MDA-231 cell lines. Cytotoxicity assays showed that emodin treatment for 48hours caused a concentration dependent decrease in viable cell numbers of both cell lines. As determined by cell counting...
Effect of dietary selenium and vitamin E on the biomechanical properties of rabbit bones
Turan, B; Balcik, C; Akkas, N (Springer Science and Business Media LLC, 1997-09-01)
It is generally agreed that combined deficiency of selenium and vitamin E leads to several abnormalities including Kashin-Beck disease which is an endemic and chronic degenerative osteoarthrosis. The abnormalities can be reversed by the administration of various forms of selenium and vitamin E.
Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase
Çoruh, Nursen; Ozgokce, F. (2007-01-01)
Therapeutic effects of several medicinal plants and vegetables, which are commonly used as food and in folk medicine against many disease, are well known. Antioxidant capacities of Heracleum persicum Desf., Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. species from Apiaceae family were evaluated by determining their effects on DPPH radical scavenging, and lipid peroxidation inhibition, as well as their total phenolic contents. Potential natural glutathione-S-transferase inhibitors have gain...
Effect of gemcitabine and retinoic acid loaded PAMAM dendrimer-coated magnetic nanoparticles on pancreatic cancer and stellate cell lines
Yalcin, Serap; Erkan, Mert; Unsoy, Gozde; Parsian, Maryam; Kleeff, Jorg; Gündüz, Ufuk (2014-07-01)
Gemcitabine is an anticancer drug used in the treatment of different cancer types, including pancreatic ductal adenocarcinoma. The maximum tolerated dose in humans is restricted by its side effects on healty cells. Furthermore, the fibrotic stroma produced by the pancreatic stellate cells prevents effective delivery of chemotherapeutic agents providing a safe-haven for the cancer cells. This becomes more of a problem considering the short half-life of this drug. Magnetic nanoparticle-based targeted drug del...
Anticarcinogenic effects of the ethanolic extract from salix aegyptiaca l. in colon cancer, involvement of akt pathway
Enayat, Shabnam; Banerjee, Sreeparna; Department of Biology (2013)
Salix aegyptiaca has been used for centuries as a medicinal plant for various health disorders. Our previous study demonstrated high contents of phenolics and antioxidants in its bark many of those are famed for their anti-inflammatory properties. Since inflammation frequently accompanies the progress of colorectal cancer, in the current study we showed that the ethanolic extract of bark (EEB) from S.aegyptiaca exerted strong anti-proliferative effects on several cancer cells but not on non-cancerous CCD-18...
Citation Formats
B. Kaya, “Effect of emodin on doxorubicin-induced cytotoxicity and apoptosis on MCF-7 and MCF-10a cells,” M.S. - Master of Science, Middle East Technical University, 2014.