Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice by Rhodobacter Capsulatus

Download

index.pdf

Date

2011

Author

Özkan, Endam

Metadata

Show full item record

Item Usage Stats

210
views

101
downloads

Biological hydrogen production through integration of dark and photo-fermentation by using biomass is a promising alternative for energy supply problems. The main purpose of this study was to investigate photobiological H2 production by the purple non-sulfur (PNS) bacteria Rb. capsulatus on dark fermentation effluent of sugar beet thick juice (DFESBTJ). Presence of NH4+ in effluents is an important parameter since NH4+ inhibit the nitrogenase enzyme activity. Therefore, the influence of different NH4+ concentrations in the DFESBTJ by removing using natural zeolite clinoptilolite on photofermentative H2 production were studied using Rb. capsulatus DSM1710 and Rb. capsulatus YO3 (hup-). Also, the effect of EtOH concentrations (between 6.25 and 200 mM) in the defined medium on H2 production were studied using both bacterial strains since EtOH is a possible by-product of dark fermentation process. The experiments were carried out in small scale bottle photobioreactors (PBRs) and outdoor panel PBR (4 L). H2 productivity of 1.12 mmol/Lc/h was attained over 15 days of operation for panel PBR. The results showed that the zeolite was effective in removing NH4+ from the DFESBTJ as its concentration decreased by 95% after treatment. In both bacterial strains, an increase in the maximum productivities and molar H2 yields was observed with the decrease in NH4+concentrations. There was no significant effect of EtOH on H2 production except the inhibition at 200 mM. The main conclusions were that both bacterial strains could effectively utilize the DFESBTJ for growth and H2 production, therefore facilitating the integration of the dark and photo-fermentation for sustainable biohydrogen production.

Subject Keywords

Biotechnology., Hydrogen industry.

URI

http://etd.lib.metu.edu.tr/upload/12613718/index.pdf
https://hdl.handle.net/11511/20953

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Phototrophic hydrogen production by agar-immobilized Rhodobacter capsulatus Elkahlout, Kamal E. M.; Yücel, Ayşe Meral; Eroğlu, İnci; Department of Biotechnology (2011) photosynthetic bacteria is attractive field as production is fueled by solar energy. Hydrogen production potential of two photosynthetic bacteria R.capsulatus (DSM1710 wild type and R.capsulatus YO3 Hup- uptake hydrogenase deleted mutant strain) were examined in agar immobilized systems. In the present work agar and glutamate concentrations were optimized for immobilization of bacteria while feeding bacteria with 40/2-4 mM acetate/ glutamate. Immobilized bacteria produced hydrogen for 420-1428 hours coverin...
Photobiological hydrogen production by Rhodobacter sphaeroides O.U.001 by utilization of waste water from milk industry Turkarslan, S; Yigit, DO; Aslan, K; Eroğlu, İnci; Gündüz, Ufuk (1997-06-26) Hydrogen production with photosynthetic microorganisms contributes to the protection of the environment, not only in producing a clean fuel but also in waste treatment processes. In this study, Rhodobacter sphaeroides, a photosynthetic bacteria, is used in photobiological hydrogen production by using waste water from the milk industry.
Hydrogen production by different strains of Rhodobacter sphaeroides Gündüz, Ufuk; Yucel, M; Turker, L; Eroglu, L (2000-06-15) Utilisation of solar energy by photosynthetic microorganisms for H-2 production attracts much interest due to unlimited supply of energy. It is important to identify the most effective strain in terms of hydrogen production for the feasibility of the process. Four different strains of Rhodobacter sp. were grown in a water-jacketed cylindrical glass-column photobioreactor under anaerobic conditions. Growth characteristics and hydrogen production rates were determined. Comparison between strains of Rhodobacte...
Kinetic analyses of the effects of temperature and light intensity on growth, hydrogenm production and organic acid utilization by rhodobacter capsulatus Sevinç, Pelin; Gündüz, Ufuk; Department of Biotechnology (2010) Effects of temperature and light intensity on photofermentative hydrogen production by Rhodobacter capsulatus DSM1710 by use of acetic and lactic acids as substrates were studied. Experiments were conducted at 20, 30 and 38oC incubator temperatures under light intensities in the 1500 – 7000 lux range. pH of the medium and quantity of hydrogen forming together with quantity of biomass, and concentrations of acetic, lactic, formic, butyric and propionic acids in the medium were determined periodically. Growth...
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01) Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...

Citation Formats

E. Özkan, “Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice by Rhodobacter Capsulatus,” M.S. - Master of Science, Middle East Technical University, 2011.