Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Ancient DNA isolation and mitochondrial DNA analysis of human samples from Çemialo Sırtı, Batman in Southeast Anatolia
Download
index.pdf
Date
2015
Author
Yaka, Reyhan
Metadata
Show full item record
Item Usage Stats
291
views
174
downloads
Cite This
The main purpose of the study was to obtain aDNA sequences of ancient human remains in the dedicated ancient DNA (aDNA) laboratory which was established at Middle East Technical University, in 2012. For this purpose, human samples approximately dating between 600-500 BC from Çemialo Sırtı excavation site in Batman in southeast Anatolia, were employed. aDNA extraction was performed using bone and teeth samples from 9 human skeletal remains. Then the mitochondrial DNA (mtDNA) Hypervariable region I and Hypervariable region II (HVRI-HVRII) of 7 samples could be successfully amplified and their sequences were obtained. Success rates in extractions (9/9) and amplifications (7/9) were 100% and 77.8%, respectively. Postmortem nucleotide changes (misincorporations) were also detected supporting the authenticity of the sequences. aDNA sequences were used to determine mtDNA haplogroups (HPGs) of the samples. The observed percentage of each haplogroup was: HPG H=28.57%, HPG HV=14.29%, HPG M=14.29%, HPG R=28.57%, HPG U2=14.29% for the 7 Çemialo Sırtı individuals. vi When the mtDNA based haplogroup and sequence data of the present study were evaluated together with the results of some other ancient and modern populations by means of Principle Component Analysis (PCA), it was observed that Çemialo Sırtı population is relatively similar to the neareastern Neolithic population (7300 BC) from northern Syria. Continuity between the Çemialo Sırtı population (n=7) and the same Neolithic population from northern Syria (n=10) was tested by calculating the FST value between these two populations, and comparing that with the FST values generated by coalescent simulations. During the simulations, random drift was assumed to be the force of genetic differentiation between the two populations. Under the exponential growth and no growth models, continuity from Neolithic population to Çemialo Sırtı population could not be rejected. Since the sample size was small in Çemialo Sırtı population, comparative results must be taken with caution.
Subject Keywords
DNA.
,
DNA, Fossil.
,
Mitochondrial DNA.
,
Biomolecules, Fossil.
URI
http://etd.lib.metu.edu.tr/upload/12618888/index.pdf
https://hdl.handle.net/11511/24794
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
A Short fragment of ancient DNA and its use in determination of sheep mitochondrial dna haplogroups in Southeast Anatolia
Dağtaş, Nihan Dilşad; Togan, İnci Zehra; Department of Biology (2013)
Recent archaeozoological studies indicated that perhaps the oldest (11,000 years before present) and may be the only sheep domestication center was in Southeast Anatolia. In this study, to contribute to the understanding of sheep domestication history, ancient DNA derived from skeletal remains of sheep unearthed from archaeological sites in Turkey mainly from Oylum Höyük in Kilis were examined. 187 ancient metapodia and mandible samples, dating between 1,800-30 BCE were brought from Oylum Höyük to the dedic...
MTDNA based genetic diversity of native sheep breeds and Anatolian mouflon (Ovis Gmelini Anatolica)
Demirci, Sevgin; Togan, İnci Zehra; Department of Bioinformatics (2012)
In the present study, history of domestic sheep were investigated by mitochondrial DNA (mtDNA) based haplogroups (HPG) of 628 samples and mtDNA control region (CR) sequences of 240 samples from 13 Turkish sheep breeds which were located in the hearth of the first domestication center. Also, 30 Anatolian wild sheep (Ovis gmelinii anatolica) mtDNA CR sequences were obtained to contribute to the scenarios on initial domestication stages of sheep. Haplogroup compositions of breeds were identified with SSCP meth...
Genetic structure of modern and ancient swordfish populations from coasts of Turkey
Yüncü, Eren; Togan, İnci Zehra; Department of Biology (2017)
In this study, partial mitochondrial DNA (mtDNA) and 8 microsatellite loci of swordfish collected from Northern Levantine Sea and Northern Aegean Sea were analyzed. Also same mtDNA region of ancient swordfish samples unearthed from Yenikapı excavation were sequenced. Obtained sequences were evaluated comparatively with the sequences from Balearic Sea, Ligurian Sea, Ionian Sea and Southern Aegean Sea, available in databases. Analysis of the molecular variance revealed a significant differentiation between We...
Ancient genomics in Anatolia: challenges and opportunities
Somel, Mehmet (null; 2019-10-19)
The increasing availability of ancient genomes has been accelerating studies in a number of fields, including human history, molecular evolution, and conservation biology. The METU/Hacettepe Ancient DNA Group has been contributing to these endeavors by producing and analyzing ancient DNA data from archaeological human and animal remains of Anatolian origin, from the last 10,000 years. However, the temperate climate conditions across most parts of Anatolia drive rapid DNA degradation, and most biological mat...
Ancient genomics in Neolithic Central Anatolia and Çatalhöyük
Yaka, Reyhan; Doğu, Ayça; Kaptan, Damla; Dağtaş, Nihan Dilşad; Chyleński, Maciej; Vural, Kıvılcım Başak; Altınışık, Nefize Ezgi; Mapelli, Igor; Koptekin, Dilek; Karamurat, Cansu; Gemici, Hasan Can; Yorulmaz, Sevgi; Lagerholm, Vendela Kempe; Fer, Evrim; Işıldak, Ulaş; Ghalichi, Ayshin; Kılınç, Gülşah Merve; Mazzucato, Camilla; Juras, Anna; Marciniak, Arkadiusz; Larsen, Clark S.; Pilloud, Marin; Haddow, Scott D.; Knüsel, Christopher J.; Togan, İnci; Götherström, Anders; Erdal, Yılmaz Selim; Sürer, Elif; Özer, Füsun; Atakuman, Çiğdem; Somel, Mehmet (British Institute of Archaeology at Ankara, 2021-01-01)
Over multiple millennia, from the earliest traces of long-term occupation of camp sites (ca 20,000 BC) to the development of full-scale farming (ca 8000–6000 BC), the Neolithic transition in southwest Asia gradually shaped human societies in dramatic ways (Nadel 2002; Maher et al. 2012; Asouti, Fuller 2013). Here we present recent insights from ancient genomics studies into these societies while focusing on two questions: the population processes driving cultural change in Neolithic central Anatolia and gen...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
R. Yaka, “Ancient DNA isolation and mitochondrial DNA analysis of human samples from Çemialo Sırtı, Batman in Southeast Anatolia,” M.S. - Master of Science, Middle East Technical University, 2015.
