Borate modified bioglass containing scaffolds for dental tissue engineering applications

Download
2018
Moonesi Rad, Reza
Dental tissue engineering has gained increasing attention in recent years. Dentures or implants are used as replacements of the lost teeth. High cost of these restorations and rejection possibility of the implants are their main drawbacks. For this reason, a regenerative approach for repairing of the damaged dentin-pulp complex or generating a new tissue is needed. The properties associated with bioglass like biocompatibility and bioactivity are very attractive for dental applications. In this thesis, bioactive glass nanoparticles (BG-NPs) modified with borate at different ratios (7%, 14%, and 21%) were synthesized using combination of sol-gel/co-precipitation and quick alkali-mediated sol-gel methods. Their effect on the odontogenic differentiation of human dental pulp stem cells (hDPSCs) was investigated. Additionally, 3D cellulose acetate/pullulan/gelatin (CA/PULL/GEL) based dentin-like constructs containing 10 or 20% BG-NPs was studied to explore their potential for dentin regeneration. SEM and XRD analyses showed that synthesized BGs were nano-sized and amorphous in nature, respectively. Characteristic functional groups of BGs were observed in FTIR and FT-Raman spectral analysis. ICP analysis revealed that BGs synthesized possessed the expected and compositions close to the theoretical ones. The purpose of modification of bioglass with borate was to achieve increased biodegradability. Increase of B2O3 in BG content positively affected the bioactivity of BG. Higher ion release in SBF resulted in higher amounts of calcium phosphate deposited on BG discs. Three-dimensional porous scaffolds with tubular pore structures were successfully produced using two methods: thermally induced phase separation/porogen leaching (TIPS/PL) and freeze-drying/metal mold pressing (FD/MMP) and crosslinked with glutaraldehyde. KCl was added as porogen for increasing porosity of the scaffolds in TIPS/PL method. Bioactivity tests showed apatite like depositions on scaffolds. BG addition enhanced bioactivity of the scaffolds. Furthermore, borate modification resulted in higher deposition than observed in scaffolds with BG. Scaffolds without BG possessed the highest porosity percentage. Addition of 10% BG improved the mechanical properties of scaffolds. For cell culture studies hDPSCs were isolated from patient’s extracted tooth and flow cytometry analysis showed that isolated hDPSCs were mesenchymal origin. 6.25 mg/ml was chosen as the optimum BG concentration from dose dependent cytotoxicity study and used for differentiation tests. All of BG groups enhanced odontogenic differentiation of hDPSCs and presence of borate increased cell viability. hDPSCs proliferated on scaffolds prepared by both methods and presence of borate modified BGs increased cell viability observed on scaffolds. Immunohistochemical and histochemical stainings showed that scaffolds positively affected the odontoblastic differentiation of the hDPSCs. In this thesis, results showed that borate modified BG-NPs and dentin-like constructs hold promise for use in dental tissue engineering applications.

Suggestions

Bioactive agent loaded hydrogel systems for dental tissue engineering applications
Atila, Deniz Hazal; Tezcaner, Ayşen; HASIRCI, VASIF; Department of Engineering Sciences (2021-9-07)
Use of tissue engineered oral restoration products is currently a popular approach for treating dental defects that adversely affect oral health in ageing populations. Among scaffolds composed of long-lasting porous ceramics and biodegradable natural or synthetic polymers with varying service lives, injectable hydrogels attract attention to regenerate dental pulp due to the capability of filling non-uniform voids such as pulp cavity. In this study, two types of injectable hydrogels were formulized by design...
Visual attributes of products as the factors influencing Users' emotional reactions: a study on dental instruments
Çınar Okuşluğ, Kıvılcım; Ünlü, Canan Emine; Department of Industrial Design (2014)
Despite the technological advances in the field of dentistry, it is asserted that the instruments that are used in treatments may be one of the reasons of negative emotions that are still effective on dental patients. However, studies that investigate how dental instruments trigger emotions of patients are so scarce. In this study that aims to overcome this deficiency, it is tried to show relationship between product appearance and individual’s emotional response on dental instruments. This thesis dwells on...
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Structural and biological assessment of boron doped bioactive glass nanoparticles for dental tissue applications
Rad, Rezai Moonesi; Alshemary, Ammar Zeıdan Ghaılan; Evis, Zafer; Keskin, Dilek; Altunbas, Korhan; Tezcaner, Ayşen (2018-06-01)
In this article, bioactive glass nanoparticles (BG-NPs) doped with boron were synthesized and characterized to evaluate their effects on human dental pulp stem cells (hDPSCs). All synthesized BGs were nano-sized and amorphous in nature. They showed the expected characteristic functional groups and composition close to the designed ones by microstructural characterizations. Porositimetry analysis revealed that increase of boron in the BG composition caused a decrease in the specific surface area, average por...
Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering
Monirizad, Mahsa; Keskin, Dilek; Ermiş Şen, Menekşe; Department of Engineering Sciences (2022-2)
The need for more effective tissue grafts for orthopedic applications is one of the main research areas of tissue engineering. In bone tissue engineering (BTE), scaffolds that can mimic bone tissue both from mechanical and biological perspectives are investigated mostly. In this study, it was aimed to develop a BTE scaffold that can mimic bone ECM, mechanical strength and cell biocompatibility in a single design and thus, various groups of scaffolds were characterized in terms of mechanical, biocompatibilit...
Citation Formats
R. Moonesi Rad, “Borate modified bioglass containing scaffolds for dental tissue engineering applications,” Ph.D. - Doctoral Program, Middle East Technical University, 2018.