Structural and biological assessment of boron doped bioactive glass nanoparticles for dental tissue applications

Date

2018-06-01

Author

Rad, Rezai Moonesi
Alshemary, Ammar Zeıdan Ghaılan
Evis, Zafer
Keskin, Dilek
Altunbas, Korhan
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

284
views

0
downloads

In this article, bioactive glass nanoparticles (BG-NPs) doped with boron were synthesized and characterized to evaluate their effects on human dental pulp stem cells (hDPSCs). All synthesized BGs were nano-sized and amorphous in nature. They showed the expected characteristic functional groups and composition close to the designed ones by microstructural characterizations. Porositimetry analysis revealed that increase of boron in the BG composition caused a decrease in the specific surface area, average pore diameter and total pore volume of NPs. hDPSCs were isolated from third molar teeth of patients and were shown to have the characteristics of mesenchymal stem cells. Dose dependent cytotoxicity study of boron doped BG-NPs suggested that 6.25 mg/ml was the optimum concentration for cells. ALP activity tests and intracellular calcium measurements revealed enhanced early stage odontogenic differentiation of hDPSCs treated with 6.25 mg/ml of different BG groups. Immunocytochemical staining showed positive effect of boron doped BG-NPs on DSPP, osteopontin and collagen I markers expression of hDPSCs. Our results indicated that boron doped BG-NPs hold potential as biomaterial in regenerative dentistry.

Subject Keywords

Dental tissue engineering, Nano bioactive glass, Boron, Dental pulp stem cells, Regenerative dentistry

URI

https://hdl.handle.net/11511/48155

Journal

CERAMICS INTERNATIONAL

DOI

https://doi.org/10.1016/j.ceramint.2018.02.230

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Biocompatibility of Accelerated Mineral Trioxide Aggregate on Stem Cells Derived from Human Dental Pulp. Kulan, Pinar; Karabiyik, Ozge; KÖSE, GAMZE; Kargul, Betul (2016-02-01) The aim of this study was to evaluate the effects of several additives on the setting time and cytotoxicity of accelerated-set mineral trioxide aggregate (MTA) on stem cells of human dental pulp. ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Johnson City, TN) was mixed with various additives including distilled water, 2.5% disodium hydrogen phosphate (Na2HPO4) (Merck, Darmstadt, Germany), K-Y Jelly (Johnson & Johnson, Markham, ON, Canada), and 5% and 10% calcium chloride (CaCl2) (Merck). The setting time...
FABRICATION OF MAGNETIC BIOACTIVE GLASS NANOPARTICLES Taşar, Cansu; Ercan, Batur; Department of Metallurgical and Materials Engineering (2022-8-18) Different compositions of bioactive glass nanoparticles have been investigated for various applications, including cancer treatment, drug delivery, bone regeneration, etc. However, targeting of bioactive glass nanoparticles to desired tissues still remains to be a challenge. In this research, sol-gel synthesized bioactive glass and superparamagnetic iron oxide nanoparticles (SPIONs) were combined using two different approaches to obtain magnetic bioactive glass nanoparticle composites. In the first ap...
Structural, mechanical, and biocompatibility investigations of yttrium and fluoride doped nano hydroxyapatite Burçin, Başar; Evis, Zafer; Department of Engineering Sciences (2009) In this study, it was aimed to investigate the structural, mechanical and biological properties of nano hydroxyapatite (HA) doped with yttrium and fluoride with different compositions. HAs were synthesized by precipitation method. After sintering at 900oC, 1100oC or 1300oC for 1 hour, the structural properties of HAs were investigated by XRD, FTIR spectroscopy and SEM. High relative densities (above 88 % of relative density) were achieved after sintering. No second phases were observed in XRD measurements. ...
Development of a novel functionally graded membrane containing boron-modified bioactive glass nanoparticles for guided bone regeneration Rad, Reza Moonesi; Atila, Deniz; Evis, Zafer; Keskin, Dilek; Tezcaner, Ayşen (2019-08-01) Barrier membranes are used in periodontal tissue engineering for successful neo-bone tissue formation and prevention of bacterial colonization. We aimed to prepare and characterize novel 7% boron-modified bioactive glass (7B-BG) containing bilayered membrane for this end. We hypothesized that presence of 7B-BG could promote structural and biological properties of guided bone regeneration (GBR) membrane. Cellulose acetate (CA) layer was prepared by solvent casting, and functionally graded layer of CA/gelatin...
Visual attributes of products as the factors influencing Users' emotional reactions: a study on dental instruments Çınar Okuşluğ, Kıvılcım; Ünlü, Canan Emine; Department of Industrial Design (2014) Despite the technological advances in the field of dentistry, it is asserted that the instruments that are used in treatments may be one of the reasons of negative emotions that are still effective on dental patients. However, studies that investigate how dental instruments trigger emotions of patients are so scarce. In this study that aims to overcome this deficiency, it is tried to show relationship between product appearance and individual’s emotional response on dental instruments. This thesis dwells on...

Citation Formats

R. M. Rad, A. Z. G. Alshemary, Z. Evis, D. Keskin, K. Altunbas, and A. Tezcaner, “Structural and biological assessment of boron doped bioactive glass nanoparticles for dental tissue applications,” CERAMICS INTERNATIONAL, pp. 9854–9864, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48155.