Structural and biological assessment of boron doped bioactive glass nanoparticles for dental tissue applications

Rad, Rezai Moonesi
Alshemary, Ammar Zeıdan Ghaılan
Evis, Zafer
Keskin, Dilek
Altunbas, Korhan
Tezcaner, Ayşen
In this article, bioactive glass nanoparticles (BG-NPs) doped with boron were synthesized and characterized to evaluate their effects on human dental pulp stem cells (hDPSCs). All synthesized BGs were nano-sized and amorphous in nature. They showed the expected characteristic functional groups and composition close to the designed ones by microstructural characterizations. Porositimetry analysis revealed that increase of boron in the BG composition caused a decrease in the specific surface area, average pore diameter and total pore volume of NPs. hDPSCs were isolated from third molar teeth of patients and were shown to have the characteristics of mesenchymal stem cells. Dose dependent cytotoxicity study of boron doped BG-NPs suggested that 6.25 mg/ml was the optimum concentration for cells. ALP activity tests and intracellular calcium measurements revealed enhanced early stage odontogenic differentiation of hDPSCs treated with 6.25 mg/ml of different BG groups. Immunocytochemical staining showed positive effect of boron doped BG-NPs on DSPP, osteopontin and collagen I markers expression of hDPSCs. Our results indicated that boron doped BG-NPs hold potential as biomaterial in regenerative dentistry.


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Barrier membranes are used in periodontal tissue engineering for successful neo-bone tissue formation and prevention of bacterial colonization. We aimed to prepare and characterize novel 7% boron-modified bioactive glass (7B-BG) containing bilayered membrane for this end. We hypothesized that presence of 7B-BG could promote structural and biological properties of guided bone regeneration (GBR) membrane. Cellulose acetate (CA) layer was prepared by solvent casting, and functionally graded layer of CA/gelatin...
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Despite the technological advances in the field of dentistry, it is asserted that the instruments that are used in treatments may be one of the reasons of negative emotions that are still effective on dental patients. However, studies that investigate how dental instruments trigger emotions of patients are so scarce. In this study that aims to overcome this deficiency, it is tried to show relationship between product appearance and individual’s emotional response on dental instruments. This thesis dwells on...
Citation Formats
R. M. Rad, A. Z. G. Alshemary, Z. Evis, D. Keskin, K. Altunbas, and A. Tezcaner, “Structural and biological assessment of boron doped bioactive glass nanoparticles for dental tissue applications,” CERAMICS INTERNATIONAL, pp. 9854–9864, 2018, Accessed: 00, 2020. [Online]. Available: