Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering

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2022-2

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Monirizad, Mahsa

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The need for more effective tissue grafts for orthopedic applications is one of the main research areas of tissue engineering. In bone tissue engineering (BTE), scaffolds that can mimic bone tissue both from mechanical and biological perspectives are investigated mostly. In this study, it was aimed to develop a BTE scaffold that can mimic bone ECM, mechanical strength and cell biocompatibility in a single design and thus, various groups of scaffolds were characterized in terms of mechanical, biocompatibility, and osteogenic properties. Poly (lactic acid) was used to 3D print main scaffold frame with different internal architectures. Two sets of experiments were designed in this thesis, i) 10 different geometries were chosen according to their porosity and pore structures, alkali treated and coated with type I collagen and bioglass (BG) nanoparticles, to mimic organic and mineral matrix of the bone, ii) 3D scaffolds with 3 different geometries were selected and filled with Collagen, 0.5% BG and cell laden GelMA hydrogel to provide an interconnected cell migration and proliferation network. The 3D printed PLA scaffolds used in the first set of experiments, in general, displayed good biocompatibility, cell adhesion, proliferation, and differentiation. Moreover, the candidate 3D scaffolds in both sets, successfully matched the mechanical properties of the trabecular bone. Voronoi-type scaffolds presented better elastic modulus, yield strength, cell proliferation and migration both in GelMA filled and collagen-coated scaffolds compared to other geometries. The osteogenic characterization of alkali modified Collagen-BG coated scaffolds, showed better results compared to untreated scaffolds.

Subject Keywords

Bone tissue engineering, 3D printing, Scaffold architecture, Composite coating, Methacrylated gelatin

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https://hdl.handle.net/11511/96248

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

M. Monirizad, “Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2022.