Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering

Monirizad, Mahsa
The need for more effective tissue grafts for orthopedic applications is one of the main research areas of tissue engineering. In bone tissue engineering (BTE), scaffolds that can mimic bone tissue both from mechanical and biological perspectives are investigated mostly. In this study, it was aimed to develop a BTE scaffold that can mimic bone ECM, mechanical strength and cell biocompatibility in a single design and thus, various groups of scaffolds were characterized in terms of mechanical, biocompatibility, and osteogenic properties. Poly (lactic acid) was used to 3D print main scaffold frame with different internal architectures. Two sets of experiments were designed in this thesis, i) 10 different geometries were chosen according to their porosity and pore structures, alkali treated and coated with type I collagen and bioglass (BG) nanoparticles, to mimic organic and mineral matrix of the bone, ii) 3D scaffolds with 3 different geometries were selected and filled with Collagen, 0.5% BG and cell laden GelMA hydrogel to provide an interconnected cell migration and proliferation network. The 3D printed PLA scaffolds used in the first set of experiments, in general, displayed good biocompatibility, cell adhesion, proliferation, and differentiation. Moreover, the candidate 3D scaffolds in both sets, successfully matched the mechanical properties of the trabecular bone. Voronoi-type scaffolds presented better elastic modulus, yield strength, cell proliferation and migration both in GelMA filled and collagen-coated scaffolds compared to other geometries. The osteogenic characterization of alkali modified Collagen-BG coated scaffolds, showed better results compared to untreated scaffolds.


3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering
Yedekçi, Buşra; Evis, Zafer; Tezcaner, Ayşen; Department of Engineering Sciences (2021-9-6)
Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were s...
3D plotted PCL scaffolds for stem cell based bone tissue engineering
Yilgor, Pinar; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2007-10-04)
The ability to control the architecture and strength of a bone tissue engineering scaffold is critical to achieve a harmony between the scaffold and the host tissue. Rapid prototyping (RP) technique is applied to tissue engineering to satisfy this need and to create a scaffold directly from the scanned and digitized image of the defect site. Design and construction of complex structures with different shapes and sizes, at micro and macro scale, with fully interconnected pore structure and appropriate mechan...
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Tissue engineered cartilage on collagen and PHBV matrices
Kose, GT; Korkusuz, F; Ozkul, A; Soysal, Y; Ozdemir, T; Yildiz, C; Hasırcı, Vasıf Nejat (2005-09-01)
Cartilage engineering is a very novel approach to tissue repair through use of implants. Matrices of collagen containing calcium phosphate (CaP-Gelfix (R)), and matrices of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) were produced to create a cartilage via tissue engineering. The matrices were characterized by scanning electron microscopy (SEM) and electron diffraction spectroscopy (EDS). Porosity and void volume analysis were carried out to characterize the matrices. Chondrocytes were isola...
Surface modified multi-functional PCL/TCP fibrous scaffolds
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2016-05-17)
Porous and fibrous scaffolds prepared using various methods are essential components of tissue engineering. Poly(ε-caprolactone) (PCL), due to its biocompatibility and mechanical properties, is one of the most desirable polymers in scaffold preparation for bone tissue engineering applications, and there are many studies on modification of PCL to enhance its biocompatibility. In this study, porous and fibrous scaffolds of PCL containing TCP were prepared by wet spinning and gelatin was immobilized onto it to...
Citation Formats
M. Monirizad, “Surface functionalized poly-lactic acid (PLA) scaffolds for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2022.