A Comparison on protein, bacterial anti-adhesive and anti-bacterial properties of zwitterionic block copolymer micelle containing ultra-thin films of varying compositions

Ulusan, Sinem
In this study preparation of ultra-thin coatings of zwitterionic block copolymer micelles and a comparison of their protein adsorption, adhesiveness and anti-bacterial properties as well as adhesiveness against osteoblast-like cells were studied. Zwitterionic block copolymer micelles were obtained through pH-induced self-assembly of poly[3-dimethyl (methacryloyloxyethyl) ammonium propane sulfonate-b-2-(diisopropylamino)ethyl methacrylate] (βPDMA-b-PDPA) at pH 7.5. βPDMA-b-PDPA micelles with zwitterionic βPDMA-corona and pH-responsive PDPA-core were then used as building blocks to prepare either 1-layer or layer-by-layer (LbL) assembled multilayer films together with Hyaluronic Acid (HA), Tannic Acid (TA) or poly(sodium 4-styrene sulfonate) (PSS). Protein adsorption tests showed that 3-layer βPDMA-b-PDPA micelles/HA films were the most effective in reducing the adhesion of BSA, lysozyme, ferritin, and casein. In contrast, βPDMA-b-PDPA micelles/TA films were the most attractive surfaces for protein adsorption. Bacterial anti-adhesive tests against a model Gram-negative bacterium, Escherichia coli and a model Gram-positive bacterium, Staphylococcus aureus were in good agreement with the protein adsorption properties of the films. The differences in the anti-adhesive properties between these three different film systems were discussed within vi the context of chemical nature and the functional chemical groups of the polyanions, layer number and surface morphology of the films. Multilayers were found to lose their anti-adhesiveness in the long-term. However, by taking advantage of the pH-responsive hydrophobic micellar cores, an anti-bacterial agent could be loaded into the micelles and multilayers could exhibit anti-bacterial activity in the long-term especially at moderately acidic conditions. In contrast to anti-adhesive properties, no significant differences were recorded in the anti-bacterial properties or osteoblast adhesive properties between the different film types.


Study on the properties of crosslinking of poly(ethylene oxide) and hydroxyapatite-poly(ethylene oxide) composite
Banat, R; Tincer, T (2003-10-10)
This study covers the crosslinking of poly-(ethylene oxide) (PEO) and its composite with calcium hydroxyapatite (HA), their mechanical and swelling properties, and morphology. Sheets of the composites of PEO (two different grades with M-v: 5x10(6) and 2x10(5)) and HA and neat PEO were prepared by compression molding. The prepared composite. and. PEO (0.1-mm-thick) sheets were crosslinked with exposure of UV-irradiation in the presence of a photoinitiator, acetophenone (AP). This simple method for crosslinki...
Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle
Ince, Alev Emine; Şahin, Serpil; Şümnü, Servet Gülüm (2014-10-01)
In this study, extraction of phenolic compounds from nettle by microwave and ultrasound was studied. In both microwave and ultrasound-assisted extractions, effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid to solvent ratio (1:10, 1:20, and 1:30 g/mL) on total phenolic content (TPC) were investigated. Effects of different powers (50 % and 80 %) were also studied for ultrasound-assisted extraction. In microwave-assisted extraction, the optimum TPC of the extracts (24.64 +/...
Caglayani, Zeynep; Sel, Kaan; Yalcin, Yagmur Demircan; Sukas, Ozlem Sardan; Külah, Haluk (2017-06-22)
This study presents an approach for analyzing the dielectrophoretic (DEP) spectra of biological cells without ascertaining their membrane and cytoplasmic properties. DEP spectrum device with reciprocal V-shaped planar-electrodes was utilized to obtain DEP spectra information of cells. Integrating electric field simulations based on a strong theory with the experimental data enables determination of the DEP spectrum at a range of frequencies, for the first in the literature. The proposed method is tested wit...
Comparison of antioxidant enzyme activities and DNA damage in chickpea (Cicer arietinum L.) genotypes exposed to vanadium
Imtiaz, Muhammad; Mushtaq, Muhammad Adnan; Rizwan, Muhammad Shahid; Arif, Muhammad Saleem; Yousaf, Balal; Ashraf, Muhammad; Xiong Shuanglian, Xiong Shuanglian; Rizwan, Muhammad; Mehmood, Sajid; Tu, Shuxin (2016-10-01)
The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L-1 induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L-1 and membrane was also damaged. It was...
Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10)
The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...
Citation Formats
S. Ulusan, “A Comparison on protein, bacterial anti-adhesive and anti-bacterial properties of zwitterionic block copolymer micelle containing ultra-thin films of varying compositions,” M.S. - Master of Science, Middle East Technical University, 2019.