Immobilization of invertase in functionalized copolymer matrices

Date

2000-10

Author

Erginer, Reyhan
Toppare, Levent Kamil
Alkan, Selmiye
Bakir, Ufuk

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In this study, immobilization of invertase on functionalized polymer electrodes constructed with pyrrole-capped polyazotetrahydrofuran and polytetrahydrofuran-block-polystyrene copolymer matrices was performed. Immobilization in these enzyme electrodes was carried out by file entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole. Sodium dodecyl sulphate was used as the supporting electrolyte in the preparation of enzyme electrodes. The effects of temperature and pH on the activity of the enzyme electrodes were examined, and re-use number studies were performed. The changes in the maximum reaction rate and the variations of the Michaelis-Menten constant were investigated.

Subject Keywords

Immobilization, Invertase, Functionalized polyazotetrahydrofuran, Functionalized polytetrahydrofuran-block-polystyrene

URI

https://hdl.handle.net/11511/28494

Journal

Reactive and Functional Polymers

DOI

https://doi.org/10.1016/s1381-5148(00)00036-5

Collections

Department of Chemistry, Article

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Immobilization of invertase in conducting polypyrrole polytetrahydrofuran graft polymer matrices Kizilyar, N; Akbulut, Ural; Toppare, Levent Kamil; Ozden, MY; Yagci, Y (1999-06-24) Four different polypyrrole/polytetrahydrofuran/invertase electrodes were constructed by the entrapment of invertase in conducting polymer matrices via electropolymerization. Immobilization of the enzyme was achieved by the application of a 1.1 V constant potential to a platinum electrode for 30 min in a solution containing 0.01 M pyrrole, 0.4 mg/ml invertase and 0.4 mg/ml sodium dodecyl sulphate. Performance of each electrode was optimized by examining the effects of pH and temperature on the properties of ...
Immobilization of yeast cells in several conducting polymer matrices Balci, Z; Akbulut, Ural; Toppare, Levent Kamil; Alkan, S; Bakir, U; Yagci, Y (2002-01-01) Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of...
Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices Arslan, Ahu; Toppare, Levent Kamil; Department of Chemistry (2006) Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) were achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/solven...
Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole Tuncagil, Sevinc; Kiralp, Senem; Varls, Serhat; Toppare, Levent Kamil (Elsevier BV, 2008-03-01) In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (V-max) and substrate affinity (K-m), optimum temperature and pH, operational and storage stabilities of immobilized enzyme were determined.
Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices Arslan, A; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Elsevier BV, 2005-04-01) Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) was achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (V-max) and Michaelis-Menten constant were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/sol vent sy...

Citation Formats

R. Erginer, L. K. Toppare, S. Alkan, and U. Bakir, “Immobilization of invertase in functionalized copolymer matrices,” Reactive and Functional Polymers, pp. 227–233, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/28494.