Biological hydrogen production from sugar beet molasses by agar immobilized R. capsulatus in a panel photobioreactor

Koku, Harun
Gündüz, Ufuk
Yücel, Ayşe Meral
Biohydrogen production from sugar beet molasses was investigated by using agar immobilized R. capsulatus YO3. A panel photobioreactor (1.4 L) was employed for a long-term hydrogen production in both indoor and outdoor conditions. The impact of several initial molasses concentrations on hydrogen production, yield and productivity were assessed. Indoor studies revealed that initial sucrose concentration in molasses should be kept below 20 mM to prevent inhibition of hydrogen production. The highest hydrogen productivity of 0.64 +/- 0.06 mmol H-2 L-1 h(-1) and yield of 12.2 +/- 1.5 mol H-2/mol sucrose were obtained in indoors throughout 20 days of operation. For outdoors, hydrogen production continued for 40 days including consecutive 10 rounds under natural outdoor conditions. In outdoor conditions, the maximum hydrogen productivity and yield were 0.79 +/- 0.04 mmol H-2 L-1 h(-1) and 5.2 +/- 0.4 mol H-2/mol sucrose respectively. These results indicate that the proposed system is promising for biohydrogen production from molasses at large-scale natural conditions. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.


Continuous hydrogen production by Rhodobacter sphaeroides O.U.001
Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26)
This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.
Photoproduction of hydrogen from sugar refinery wastewater by Rhodobacter sphaeroides OU 001
Yetis, M; Gündüz, Ufuk; Eroglu, I; Yucel, M; Turker, L (2000-11-01)
Pretreated sugar refinery wastewater (SRWW) was used for the production of hydrogen by Rhodobacter sphaeroides O.U.001 in a 0.4 1 column photobioreactor. Hydrogen was produced at a rate of 0.001 1 hydrogen/h/l culture in 20% dilution of SRWW. To adjust the carbon concentration to 70 mM and nitrogen concentration to 2 mM, sucrose or l-malic acid was added as carbon source and sodium glutamate was added as nitrogen source to the 20% dilution of SRWW. By these adjustments, hydrogen production rate was increase...
Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor
Elkahlout, Kamal; Alipour, Siamak; Eroglu, Inci; Gündüz, Ufuk; Yücel, Ayşe Meral (2017-04-01)
In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best result...
Recombinant protein production by sucrose-utilizing Escherichia coli W: untreated beet molasses-based feeding strategy development
Akdag, Burcu; Çalık, Pınar (2015-06-01)
BACKGROUNDUntreated beet molasses feeding strategy was developed for recombinant thermostable glucose isomerase (GI) production by sucrose-utilizing Escherichia coli W.
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent
Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01)
Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Citation Formats
K. ELKAHLOUT et al., “Biological hydrogen production from sugar beet molasses by agar immobilized R. capsulatus in a panel photobioreactor,” 2018, Accessed: 00, 2020. [Online]. Available: