In vitro investigation and biomechanical modeling of the effects of PLF-68 on osteoarthritis in a three-dimensional model

Date

2011-10-01

Author

Kavas, Aysegul
Ozdemir, Mustafa
Gürses, Senih
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

181
views

0
downloads

In this study, it was hypothesized that Pluronic F-68 (PLF-68) increases matrix synthesis of osteoarthritis (OA) chondrocytes in addition to its well-documented cell survival effect. To test this hypothesis, rat articular chondrocytes were embedded in agarose discs and were exposed to 5-azacytidine (Aza-C) to induce OA-like alterations. Chondrocytes were then treated with PLF-68 (8 and 12 mg/ml) for 10 days. Aza-C-exposed and PLF-68-untreated chondrocytes and Aza-C-unexposed and PLF-68-untreated chondrocytes were used as negative and positive control groups, respectively. Dynamic hydrostatic pressure (max 0.2 MPa, 0.1 Hz) was applied to discs for 30 min/day (5 days/week). Cell viability, collagen and proteoglycan deposition in discs were determined. Unconfined compression stress relaxation tests were performed to determine peak stress and material parameters of discs-namely spring constants (k (1) and k (2)), damping coefficient (eta), instantaneous modulus (E (0)) and relaxed modulus (E (a)) using Kelvin model to evaluate the functional coherence of the matrix. PLF-68 treatment significantly increased the collagen deposition in discs and viability of OA-like chondrocytes. A dose-dependent increase was also observed for elastic stiffness parameters (k (1), k (2), E (0) and E (a)). Same positive effect of PLF-68 was not observed for proteoglycan deposition. However, dose-dependent increase in eta suggests that PLF-68 treatment resulted with the deposition of functional matrix. This is the first study which reports that PLF-68 has also positive effect on collagen synthesis of OA cells. As a conclusion, our results suggest that PLF-68 has a potential for recovery from OA-like alterations, which should be further analyzed.

Subject Keywords

Osteoarthritis, Pluronic F-68, Collagen, Proteoglycan, Kelvin model

URI

https://hdl.handle.net/11511/33153

Journal

BIOMECHANICS AND MODELING IN MECHANOBIOLOGY

DOI

https://doi.org/10.1007/s10237-010-0262-2

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

In vitro and in vivo evaluation of the effects of demineralized bone matrix or calcium sulfate addition to polycaprolactone-bioglass composites ERDEMLİ, ÖZGE; Captug, O.; Bilgili, H.; ORHAN, DİCLEHAN; Tezcaner, Ayşen; Keskin, Dilek (Springer Science and Business Media LLC, 2010-01-01) The objective of this study was to improve the efficacy of polycaprolactone/bioglass (PCL/BG) bone substitute using demineralized bone matrix (DBM) or calcium sulfate (CS) as a third component. Composite discs involving either DBM or CS were prepared by compression moulding. Bioactivity of discs was evaluated by energy dispersive X-ray spectroscopy (ESCA) and scanning electron microscopy (SEM) following simulated body fluid incubation. The closest Calcium/Phosphate ratio to that of hydroxyl carbonate apatit...
An investigation of microstructure, microhardness and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride Toker, Sıdıka Mine; Evis, Zafer; Department of Biomedical Engineering (2010) The aim of this study was to investigate the microstructure, microhardness and biocompatibility properties of nano hydroxyapatite (HA) doped with a constant yttrium (Y3+) and varying fluoride (F-) compositions. HA was synthesized via precipitation method and sintered at 1100C for 1 hour. Increased densities were achieved upon Y3+ doping while F- doping led to a decrease in densities. For structural analysis, XRD, SEM and FTIR spectroscopy examinations were performed. No secondary phases were observed in XR...
In vivo response to biodegradable controlled antibiotic release systems Korkusuz, F; Korkusuz, P; Eksioglu, F; Gursel, I; Hasırcı, Vasıf Nejat (2001-05-01) In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ra...
In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159 Akkaya, Mahinur; Dagdas, Gulay Gok; Dagdas, Yasin F. (2011-09-01) One of the molecular mechanisms underlying the plant–pathogen interactions is the regulation of gene expressions in plant responses by microRNAs which are either stimulated or silenced against pathogen attacks. Among the plant miRNAs, we found that mir159 is one of which that differentially expressed upon Blumeria graminis f. sp. hordei (Bgh) infected resistant and susceptible barley lines. The study aims to confirm its role in regulating one of its putative target genes, GaMyb transcription factor, in vivo...
The effect of arginine-428 mutation on modulation of activity of human liver flavin monooxygenase 3 (FMO3) by imipramine and chlorpromazine Adalı, Orhan; Philpot, RM (1999-07-01) This study was carried out to investigate the molecular basis for modulation of recombinant FMO3-catalyzed activity by the tricyclicantidepressants, imipramine and chlorpromazine. A mutant of human liver FMO3 (T428R) was formed by site-directed mutagenesis and characterized along with the native enzyme in order to elucidate a possible structure-function relationship. Functional properties of native and T428R human FMO3s were studied with methimazole as substrate. Both enzymes catalyzed the S-oxidation of me...

Citation Formats

A. Kavas, M. Ozdemir, S. Gürses, D. Keskin, and A. Tezcaner, “In vitro investigation and biomechanical modeling of the effects of PLF-68 on osteoarthritis in a three-dimensional model,” BIOMECHANICS AND MODELING IN MECHANOBIOLOGY, pp. 641–650, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/33153.