IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING

Date

1992-01-01

Author

BAYRAMOGLU, Z
Akbulut, Ural
SUNGUR, S

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Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.

Subject Keywords

Biophysics, Mechanics of Materials, Bioengineering, Biomaterials, Ceramics and Composites

URI

https://hdl.handle.net/11511/36325

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/0142-9612(92)90131-7

Collections

Department of Chemistry, Article

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IMMOBILIZATION OF ALPHA-AMYLASE INTO GELATIN FILMS WITH VARIOUS CROSS-LINKERS BAYRAMOGLU, Z; Akbulut, Ural; SUNGUR, S (1992-05-01) This article reports the cross linking of alpha-amylase enzyme to gelatin by using formaldehyde, chromium (III) acetate, chromium (III) sulfate and potassium chromium (III) sulfate as hardeners. The effect of concentrations of cross linkers, enzyme and gelatin on the activity of immobilized enzyme were investigated.
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Citation Formats

Z. BAYRAMOGLU, U. Akbulut, and S. SUNGUR, “IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING,” BIOMATERIALS, pp. 704–708, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36325.