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IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING
Date
1992-01-01
Author
BAYRAMOGLU, Z
Akbulut, Ural
SUNGUR, S
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.
Subject Keywords
Biophysics
,
Mechanics of Materials
,
Bioengineering
,
Biomaterials
,
Ceramics and Composites
URI
https://hdl.handle.net/11511/36325
Journal
BIOMATERIALS
DOI
https://doi.org/10.1016/0142-9612(92)90131-7
Collections
Department of Chemistry, Article