IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING

Date

1992-01-01

Author

BAYRAMOGLU, Z
Akbulut, Ural
SUNGUR, S

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Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.

Subject Keywords

Biophysics, Mechanics of Materials, Bioengineering, Biomaterials, Ceramics and Composites

URI

https://hdl.handle.net/11511/36325

Collections

Department of Chemistry, Article