STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING

Date

1992-01-01

Author

SUNGUR, S
ELCIN, YM
Akbulut, Ural

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Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).

Subject Keywords

Biophysics, Mechanics of Materials, Bioengineering, Biomaterials, Ceramics and Composites

URI

https://hdl.handle.net/11511/41161

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/0142-9612(92)90020-o

Collections

Department of Chemistry, Article

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Citation Formats

S. SUNGUR, Y. ELCIN, and U. Akbulut, “STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING,” BIOMATERIALS, pp. 795–800, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41161.