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STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING
Date
1992-01-01
Author
SUNGUR, S
ELCIN, YM
Akbulut, Ural
Metadata
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Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).
Subject Keywords
Biophysics
,
Mechanics of Materials
,
Bioengineering
,
Biomaterials
,
Ceramics and Composites
URI
https://hdl.handle.net/11511/41161
Journal
BIOMATERIALS
DOI
https://doi.org/10.1016/0142-9612(92)90020-o
Collections
Department of Chemistry, Article
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S. SUNGUR, Y. ELCIN, and U. Akbulut, “STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING,”
BIOMATERIALS
, pp. 795–800, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41161.