Changes in total protein profiles of barley cultivars in response to toxic boron concentration

2000-01-01
Mahboobi, H
Yucel, M
Öktem, Hüseyin Avni
In this study, ten-day-old seedlings of barley {Hordeum vulgare L. cultivar Anadolu [boron (B)-tolerant] and Hamidiye (B-sensitive)} were used. Boron-treated plants were grown on H3BO3 solution (final concentration of 10 mM) for five days. Control plants received no B treatment during this period. Total protein patterns were obtained by analysis of total protein extract from root and leaf tissues of control and B-treated plants using two-dimensional gel electrophoresis followed by silver staining. The protein profile of B-treated seedlings of each cultivar was compared to the profile of control (no stress treatment) plants of the same cultivar. Silver-stained gels showed that B stress caused increases or decreases in a number of proteins in root and leaf tissues. Moreover, as a result of B treatment, one newly synthesized protein with relative molecular weight (Mr) of 35.0 kDa was detected in root profile of the tolerant cultivar. This protein failed to show up in root profile of the B-treated sensitive cultivar. Three proteins were quantitatively increased in B-treated root profile of both cultivars. Following B treatment, three proteins were increased in root profile of the tolerant cultivar, but were not changed in the sensitive one. In leaf tissues, however, there were remarkable changes in total protein profiles after B treatment, relative to the control. Following B treatment, in leaf tissues, at least seven proteins were increased in amount in tolerant cultivar but were unchanged in the susceptible one. In tolerant and sensitive cultivars, amounts of two proteins were increased in B-treated plants, relative to control seedlings. In addition, four proteins (M:29, 58, 58, and 22 kDa) were unchanged in control and B-treated seedlings of the tolerant cultivar. In the susceptible cultivar however, among these four proteins, the first one (Mr:29) was very much reduced and the others (Mr:58, 58, and 22 kDa) were completely lost in B-treated seedlings. Moreover, following B treatment, a set of high-molecular-weight proteins was quantitatively decreased in the susceptible cultivar but was unchanged in the tolerant cultivar. These results indicate that in barley, certain proteins may be involved in tolerance to B toxicity. In this study, changes in polypeptide composition as a result of B toxic concentration in leaf tissues were more abundant than in roots. Therefore, it is suggested that these changes, especially at shoot level may form the basis of the tolerance mechanism to B toxicity.
JOURNAL OF PLANT NUTRITION

Suggestions

Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis
Çalık, Pınar; Calik, G; Ozdamar, TH (2002-10-03)
The effects of the controlled and uncontrolled pH conditions, as well as of the value of initial pH in the range of 7-7.5, on serine alkaline protease (SAP) production by Bacillus licheniformis were investigated on a defined medium with the single carbon source glucose in batch bioreactors. Besides uncontrolled pH operations, growth phase, production phase, and entire process control strategies were also applied. The concentrations of the product (SAP) and by-products, i.e. neutral protease, amylase, amino ...
Changes in Free Amino Acid and Sugar Levels of Dried Figs during Aflatoxin B(1) Production by Aspergillus flavus and Aspergillus parasiticus
Senyuva, Hamide Z.; Gilbert, John; Ozturkoglu, Sebnem; Özcan Kabasakal, Süreyya; Gurel, Neslihan (American Chemical Society (ACS), 2008-10-22)
An aqueous slurry of gamma-irradiated sterilized dried figs was inoculated with toxigenic strains of Aspergillus flavus and Aspergillus parasiticus. During incubation at 28 degrees C, pH, fructose, glucose, and free amino acids were determined by high-performance liquid chromatography and liquid chromatography (LC)/mass spectrometry, respectively, over 13 time points (1-20 days). At the same 13 time points using a LC/time-of-flight mass spectrometry screening method, aflatoxin B, and other secondary metabol...
CHANGES IN PATTERNS OF ALKALINE SERINE PROTEASE AND BACILYSIN FORMATION CAUSED BY COMMON EFFECTORS OF SPORULATION IN BACILLUS-SUBTILIS-168
BASALP, A; Özcengiz, Gülay; ALAEDDINOGLU, NG (Springer Science and Business Media LLC, 1992-03-01)
Bacilysin biosynthesis and alkaline serine protease production in Bacillus subtilis 168 were monitored and compared in batch cultures when various effectors of sporulation were added at different stages of growth in a medium containing sucrose and glutamate. Depending on the time of addition, glucose affected sporulation and serine protease formation to the same extent, but had no effect on bacilysin production. Ammonium and L-alanine additions suppressed all three processes. Casamino acids severely interfe...
Genetic characterization of pear cultivars (Pyrus communis) in Çoruh river basin
Çoban, Abdulbaki; Kaya, Zeki; Department of Molecular Biology and Genetics (2019)
The genus Pyrus contains more than 20 different pear species which are used as food source, horticultural, and ornamental purposes. Overall, pear is the second most consumed pome fruit after apple. P. communis is the most cultivated pear species in Europe and Asia Minor. However, more than 10 species of genus Pyrus can be found naturally in Europe and Asia Minor. In this study, 8 P. communis populations (one wild and 7 cultivated) and 1 P. eleagnifolia population was used to reveal genetic structure of thos...
Changes in the chemical composition of an acidic soil treated with marble quarry and marble cutting wastes
TOZSİN, Gülşen; ÖZTAŞ, Taşkın; Arol, Ali İhsan; KALKAN, Ekrem (Elsevier BV, 2015-11-01)
Soil acidity greatly affects the availability of plant nutrients. The level of soil acidity can be adjusted by treating the soil with certain additives. The objective of this study was to determine the effect of marble quarry waste (MQW) and marble cutting waste (MCW) on the chemical composition and the acidity of a soil. Marble wastes at different rates were applied to an acid soil. Their effectiveness in neutralizing the soil pH was compared with that of agricultural lime. The changes in the chemical comp...
Citation Formats
H. Mahboobi, M. Yucel, and H. A. Öktem, “Changes in total protein profiles of barley cultivars in response to toxic boron concentration,” JOURNAL OF PLANT NUTRITION, pp. 391–399, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37130.