Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G

Date

2014-05-01

Author

Erdemli, Ozge
Usanmaz, Ali
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

63
views

0
downloads

Polyester-polyether type block copolymers have attracted attention in the area of drug delivery systems with their capability in providing a broad range of amphiphilic characteristics. The aim of the present work was to prepare and characterize immunoglobulin G (IgG) loaded methoxy poly(ethylene glycol)-poly(epsilon-caprolactone)-methoxy poly(ethylene glycol) (MPEG-PCL-MPEG) microspheres as potential carrier for therapeutic monoclonal antibodies used in clinics. MPEG-PCL-MPEG triblock copolymer was synthesized by ring-opening polymerization of epsilon-caprolactone initiated by MPEG and then characterized. Microspheres were prepared by double emulsion-solvent evaporation method and their properties were compared with those of PCL microspheres. Microspheres had spherical shape with a mean particle size around 6 pm. MPEG-PCL-MPEG microspheres had higher encapsulation efficiency than PCL microspheres. After 90 days of release, 30 +/- 2% and 57 +/- 3% of the bioactivity of IgG released from non-irradiated PCL and MPEG-PCL-MPEG microspheres were protected, respectively. Presence of MPEG in microspheres provided more controlled IgG release rate and protected IgG from denaturation during gamma-irradiation (20 +/- 3% and 49 +/- 2% for PCL and MPEG-PCL-MPEG microspheres, respectively). In vitro cytotoxicity tests revealed that both MPEG-PCL-MPEG and PCL microspheres had no toxic effect on cells. This study showed that MPEG-PCL-MPEG microspheres are promising delivery systems for therapeutic monoclonal antibodies.

Subject Keywords

Biotechnology, Physical and Theoretical Chemistry, Colloid and Surface Chemistry, Surfaces and Interfaces, General Medicine

URI

https://hdl.handle.net/11511/38765

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES

DOI

https://doi.org/10.1016/j.colsurfb.2014.01.037

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

A sepiolite modified conducting polymer based biosensor SÖYLEMEZ, SANİYE; Kanik, Fulya Ekiz; Tarkuc, Simge; UDUM, YASEMİN; Toppare, Levent Kamil (Elsevier BV, 2013-11-01) A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced f...
Assembly of an antiparallel homo-adenine DNA duplex by small-molecule binding Persil Çetinkol, Özgül; JAIN, SS; HUD, NV (American Chemical Society (ACS), 2004-07-21) Molecules that reversibly bind DNA and trigger the formation of non-Watson−Crick secondary structures would be useful in the design of dynamic DNA nanostructures and as potential leads for new therapeutic agents. We demonstrate that coralyne, a small crescent-shaped molecule, promotes the formation of a duplex secondary structure from homo-adenine oligonucleotides. AFM studies reveal that the staggered alignment of homo-adenine oligonucleotides upon coralyne binding produces polymers of micrometers in lengt...
Physical Characterization of Inclusion Complexes of Triphenyl Phosphate and Cyclodextrins in Solution. Zhang, N; Zane, CP; Chen, Y; Yıldırım, Erol; Hinks, D; Tonelli, AE; Vinueza, NR; Pasquinelli, MA (American Chemical Society (ACS), 2020-01-16) The goal of this work is to provide physical insights into the formation and stability of inclusion complexes (ICs) in aqueous solution between cyclodextrins (CDs) and a common flame retardant, triphenyl phosphate (TPP). Quantum chemistry calculations reveal the possible energetically favorable geometries of TPP in their 1:1 IC form with α-, β-, and γ-CDs as well as their associated complexation, conformational, and interaction energies. High-resolution mass spectrometry (MS) and tandem MS were used with el...
The design and synthesis of a photo-controlled, peptide-based potential drug carrier Parlak Khalily, Melek; Özçubukçu, Salih (The Scientific and Technological Research Council of Turkey, 2018-01-01) Our focus in this study is on the design and synthesis of a light-responsive peptide-based nanocarrier in order to develop effective and biocompatible drug delivery systems. The synthesized nanocarrier is basically composed of peptide amphiphiles comprising a micelle forming a Pro-Pro-Pro-Lys-Lys-Lys peptide sequence with an attached anthracene fluorophore Anthracene containing an inner core of the micelle can serve as a storage site for poorly water-soluble drugs. Moreover, anthracenes that come in close p...
The role of antiferromagnetic exchange interactions in dinuclear Cr(III) dithiocarbamates and a stepwise behavior of magnetic moment AYTEKİN, ÖZLEM; Bayri, A. (Pleiades Publishing Ltd, 2012-12-01) In this study the role of the antiferromagnetic interactions in recently synthesized dinuclear Cr(III) complex has been investigated. Since there was not enough structural information for the characterization of the synthesis, we claim that there should be antiferromagnetic interactions between the dinuclear Fe(III) ions, if the proposed structure is the real structure. A new experiment is proposed to test the predictions of this theoretical investigation.

Citation Formats

O. Erdemli, A. Usanmaz, D. Keskin, and A. Tezcaner, “Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G,” COLLOIDS AND SURFACES B-BIOINTERFACES, pp. 487–496, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38765.