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Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G
Date
2014-05-01
Author
Erdemli, Ozge
Usanmaz, Ali
Keskin, Dilek
Tezcaner, Ayşen
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Polyester-polyether type block copolymers have attracted attention in the area of drug delivery systems with their capability in providing a broad range of amphiphilic characteristics. The aim of the present work was to prepare and characterize immunoglobulin G (IgG) loaded methoxy poly(ethylene glycol)-poly(epsilon-caprolactone)-methoxy poly(ethylene glycol) (MPEG-PCL-MPEG) microspheres as potential carrier for therapeutic monoclonal antibodies used in clinics. MPEG-PCL-MPEG triblock copolymer was synthesized by ring-opening polymerization of epsilon-caprolactone initiated by MPEG and then characterized. Microspheres were prepared by double emulsion-solvent evaporation method and their properties were compared with those of PCL microspheres. Microspheres had spherical shape with a mean particle size around 6 pm. MPEG-PCL-MPEG microspheres had higher encapsulation efficiency than PCL microspheres. After 90 days of release, 30 +/- 2% and 57 +/- 3% of the bioactivity of IgG released from non-irradiated PCL and MPEG-PCL-MPEG microspheres were protected, respectively. Presence of MPEG in microspheres provided more controlled IgG release rate and protected IgG from denaturation during gamma-irradiation (20 +/- 3% and 49 +/- 2% for PCL and MPEG-PCL-MPEG microspheres, respectively). In vitro cytotoxicity tests revealed that both MPEG-PCL-MPEG and PCL microspheres had no toxic effect on cells. This study showed that MPEG-PCL-MPEG microspheres are promising delivery systems for therapeutic monoclonal antibodies.
Subject Keywords
Biotechnology
,
Physical and Theoretical Chemistry
,
Colloid and Surface Chemistry
,
Surfaces and Interfaces
,
General Medicine
URI
https://hdl.handle.net/11511/38765
Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
DOI
https://doi.org/10.1016/j.colsurfb.2014.01.037
Collections
Department of Engineering Sciences, Article